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CDH1 elisa kit :: Human cadherin 1, type 1, E-cadherin (epithelial) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS704075
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $625 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,905 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

cadherin 1, type 1, E-cadherin (epithelial) (CDH1), ELISA Kit

 Also Known As   

Human E-Cadherin, E-Cad ELISA Kit

 Product Synonym Names    Human E-Cadherin; E-Cad ELISA Kit; Arc-1; CD324; CDHE; ECAD; LCAM; UVO; E-Cadherin; cadherin 1; E-cadherin (epithelial) ; cadherin 1; type 1; calcium-dependent adhesion protein; epithelial; cell-CAM 120/80; uvomorulin; cadherin 1; type 1; E-cadherin (epithelial)
 Product Gene Name   

CDH1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS704075 Testing Data    Testing Data PDF
 OMIM    gene 608089
 3D Structure    ModBase 3D Structure for P12830
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human CDH1. No significant cross-reactivity or interference between Human CDH1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Detection Range    6.25 ng/ml-400 ng/ml
 Sensitivity    1.56 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Cell Adhesion
 Protein Biological Process 3    Cell adhesion
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CDH1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CDH1 purchase    MBS704075 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cadherin 1, type 1, E-cadherin (epithelial) (CDH1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6.25 ng/ml-400 ng/ml in biological research samples containing CDH1, with an estimated sensitivity of 1.56 ng/ml. The ELISA analytical biochemical technique of the MBS704075 kit is based on CDH1 antibody-CDH1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CDH1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CDH1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for CDH1 elisa kit

   Introduction: Cadherins (Calcium dependent adhesion molecules) are a class of type-1 transmembrane proteins. They play important roles in cell adhesion, ensuring that cells within tissues are bound together. They are dependent on calcium (Ca2+) ions to function, hence their name. Different members of the cadherin family are found in different locations. E-cadherins are found in epithelial tissue; N-cadherins are found in neurons; and P-cadherins are found in the placenta. T-cadherins have no cytoplasmic domains and must be tethered to the plasma membrane. E-cadherin (epithelial) is the most well-studied member of the family. It consists of 5 cadherin repeats (EC1 ~ EC5) in the extracellular domain, one transmembrane domain, and an intracellular domain that binds p120-catenin and beta-catenin. The intracellular domain contains a highly-phosphorylated region vital to beta-catenin binding and therefore to E-cadherin function. Beta-catenin can also bind to alpha-catenin. Alpha-catenin participates in regulation of actin-containing cytoskeletal filaments. In epithelial cells, E-cadherin-containing cell-to-cell junctions are often adjacent to actin-containing filaments of the cytoskeleton. E-cadherin is first expressed in the 2-cell stage of mammalian development, and becomes phosphorylated by the 8-cell stage, where it causes compaction. In adult tissues, E-cadherin is expressed in epithelial tissues, where it is constantly regenerated with a 5-hour half-life on the cell surface. Loss of E-cadherin function or expression has been implicated in cancer progression and metastasis. E-cadherin downregulation decreases the strength of cellular adhesion within a tissue, resulting in an increase in cellular motility. This in turn may allow cancer cells to cross the basement membrane and invade surrounding tissues. E-cadherin is also used by pathologists to diagnose different kinds of breast cancer. When compared with invasive duct carcinoma, E-cadherin expression is markedly reduced or absent in the great majority of invasive lobular carcinomas when studied by immunohistochemistry.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to E-Cad. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for E-Cad and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of E-Cad in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of CDH1 elisa kit    CDH1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS704075. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CDH1. It may not necessarily be applicable to this product.
 NCBI GI #    4757960
 NCBI GeneID    999
 NCBI Accession #    NP_004351.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_004360.3 [Other Products]
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 UniProt Primary Accession #    P12830 [Other Products]
 UniProt Secondary Accession #    Q13799; Q14216; Q15855; Q16194; Q4PJ14; Q9UII8; A8K1U7 [Other Products]
 UniProt Related Accession #    P12830 [Other Products]
 Molecular Weight    97,456 Da
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 NCBI Official Full Name    cadherin-1 preproprotein
 NCBI Official Synonym Full Names    cadherin 1, type 1, E-cadherin (epithelial)
 NCBI Official Symbol    CDH1 [Similar Products]
 NCBI Official Synonym Symbols   
UVO; CDHE; ECAD; LCAM; Arc-1; CD324
[Similar Products]
 NCBI Protein Information    cadherin-1; CAM 120/80; E-Cadherin; uvomorulin; cell-CAM 120/80; epithelial cadherin; cadherin 1, E-cadherin (epithelial); calcium-dependent adhesion protein, epithelial
 UniProt Protein Name    Cadherin-1
 UniProt Synonym Protein Names   
CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CD_antigen: CD324Cleaved into the following 3 chains:E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3
 Protein Family    Cadherin
 UniProt Gene Name    CDH1 [Similar Products]
 UniProt Synonym Gene Names    CDHE; UVO; E-cadherin [Similar Products]
 UniProt Entry Name    CADH1_HUMAN
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 NCBI Summary for CDH1    This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CDH1    Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.1 PublicationManual assertion based on experiment in:Ref.27
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 Research Articles on CDH1    1. Loss of E-cadherin expression is associated with poor response to epidermal growth factor receptor-tyrosine kinase inhibitor treatment in lung cancer.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CDH1 elisa kitPathways associated with CDH1 elisa kit
 Reference Product  PubMed Publications
 CTNNB1 elisa kit  >158 publications with CDH1 and CTNNB1
 CDC27 elisa kit  >21 publications with CDH1 and CDC27
 SRC elisa kit  >11 publications with CDH1 and SRC
 CTNNA1 elisa kit  >8 publications with CDH1 and CTNNA1
 JUP elisa kit  >5 publications with CDH1 and JUP
 CBLL1 elisa kit  >2 publications with CDH1 and CBLL1
 UBC elisa kit  >1 publications with CDH1 and UBC
 CTNND1 elisa kit  >1 publications with CDH1 and CTNND1
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junctions Interactions Pathway antibodies  Adherens Junctions Interactions Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptotic Cleavage Of Cell Adhesion Proteins Pathway antibodies  Apoptotic Cleavage Of Cell Adhesion Proteins Pathway Diagram
 Apoptotic Cleavage Of Cellular Proteins Pathway antibodies  Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Arf6 Trafficking Events Pathway antibodies  Arf6 Trafficking Events Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
Diseases associated with CDH1 elisa kitOrgans/Tissues associated with CDH1 elisa kit
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >501 publications with CDH1 and Carcinoma
 Stomach Neoplasms Antibodies  >301 publications with CDH1 and Stomach Neoplasms
 Breast Neoplasms Antibodies  >243 publications with CDH1 and Breast Neoplasms
 Neoplasm Metastasis Antibodies  >158 publications with CDH1 and Neoplasm Metastasis
 Neoplasm Invasiveness Antibodies  >139 publications with CDH1 and Neoplasm Invasiveness
 Colorectal Neoplasms Antibodies  >107 publications with CDH1 and Colorectal Neoplasms
 Disease Progression Antibodies  >103 publications with CDH1 and Disease Progression
 Carcinoma, Squamous Cell Antibodies  >71 publications with CDH1 and Carcinoma, Squamous Cell
 Lung Neoplasms Antibodies  >65 publications with CDH1 and Lung Neoplasms
 Liver Neoplasms Antibodies  >63 publications with CDH1 and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Stomach Antibodies  >235 publications with CDH1 and Stomach
 Blood Antibodies  >73 publications with CDH1 and Blood
 Lymph Node Antibodies  >51 publications with CDH1 and Lymph Node
 Liver Antibodies  >48 publications with CDH1 and Liver
 Brain Antibodies  >42 publications with CDH1 and Brain
 Lung Antibodies  >40 publications with CDH1 and Lung
 Prostate Antibodies  >34 publications with CDH1 and Prostate
 Kidney Antibodies  >29 publications with CDH1 and Kidney
 Embryonic Tissue Antibodies  >25 publications with CDH1 and Embryonic Tissue
 Bladder Antibodies  >17 publications with CDH1 and Bladder
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