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CASQ1 elisa kit :: Human calsequestrin 1 (fast-twitch, skeletal muscle) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS925690
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

calsequestrin 1 (fast-twitch, skeletal muscle) (CASQ1), ELISA Kit

 Also Known As   

Human Calsequestrin-1, CASQ1 ELISA Kit

 Product Synonym Names    Human Calsequestrin-1 (CASQ1) ELISA kit; RP11-536C5.6; CASQ; PDIB1; calmitine; calsequestrin 1; calsequestrin 1 (fast-twitch; skeletal muscle)
 Product Gene Name   

CASQ1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS925690 Testing Data    Testing Data PDF
 OMIM    114250
 3D Structure    ModBase 3D Structure for P31415
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human CASQ1. No significant cross-reactivity or interference between human CASQ1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    23.5 pg/ml -1500 pg/ml.
 Sensitivity    The minimum detectable dose of human CASQ1 is typically less than 5.86 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CASQ1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CASQ1 purchase    MBS925690 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the calsequestrin 1 (fast-twitch, skeletal muscle) (CASQ1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 23.5 pg/ml -1500 pg/ml in biological research samples containing CASQ1, with an estimated sensitivity of The minimum detectable dose of human CASQ1 is typically less than 5.86 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS925690 kit is based on CASQ1 antibody-CASQ1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CASQ1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CASQ1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CASQ1 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CASQ1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CASQ1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CASQ1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CASQ1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of CASQ1 elisa kit    CASQ1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS925690. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CASQ1. It may not necessarily be applicable to this product.
 NCBI GI #    227430327
 NCBI GeneID    844
 NCBI Accession #    NP_001222.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_001231.4 [Other Products]
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 UniProt Primary Accession #    P31415 [Other Products]
 UniProt Secondary Accession #    Q8TBW7; B1AKZ2; B2R863 [Other Products]
 UniProt Related Accession #    P31415 [Other Products]
 Molecular Weight    45,160 Da
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 NCBI Official Full Name    calsequestrin-1
 NCBI Official Synonym Full Names    calsequestrin 1 (fast-twitch, skeletal muscle)
 NCBI Official Symbol    CASQ1 [Similar Products]
 NCBI Official Synonym Symbols   
CASQ; PDIB1
[Similar Products]
 NCBI Protein Information    calsequestrin-1; calmitin; calmitine; calsequestrin, skeletal muscle isoform
 UniProt Protein Name    Calsequestrin-1
 UniProt Synonym Protein Names   
Calmitin; Calsequestrin, skeletal muscle isoform
 Protein Family    Calsequestrin
 UniProt Gene Name    CASQ1 [Similar Products]
 UniProt Synonym Gene Names    CASQ [Similar Products]
 UniProt Entry Name    CASQ1_HUMAN
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 NCBI Summary for CASQ1    This gene encodes the skeletal muscle specific member of the calsequestrin protein family. Calsequestrin functions as a luminal sarcoplasmic reticulum calcium sensor in both cardiac and skeletal muscle cells. This protein, also known as calmitine, functions as a calcium regulator in the mitochondria of skeletal muscle. This protein is absent in patients with Duchenne and Becker types of muscular dystrophy. [provided by RefSeq, Jun 2013]
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 UniProt Comments for CASQ1    calsequestrin 1: Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. The skeletal muscle isoform (CASQ1) binds around 80 Ca(2+) ions, while the cardiac isoform (CASQ2) binds approximately 60 Ca(2+). Belongs to the calsequestrin family.

Protein type: Mitochondrial; Calcium-binding

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: Golgi apparatus; I band; sarcoplasmic reticulum membrane; smooth endoplasmic reticulum; sarcoplasmic reticulum lumen; sarcoplasmic reticulum; mitochondrion; mitochondrial matrix; endoplasmic reticulum; T-tubule

Molecular Function: calcium ion binding

Biological Process: endoplasmic reticulum organization and biogenesis; protein polymerization; response to organic substance; regulation of skeletal muscle contraction via regulation of the release of sequestered calcium ion; regulation of sequestering of calcium ion; skeletal muscle development; response to heat; sarcomere organization; response to denervation involved in regulation of muscle adaptation; transmembrane transport

Disease: Myopathy, Vacuolar, With Casq1 Aggregates
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 Research Articles on CASQ1    1. The sarcoplasmic reticulum calcium content in human type II fibres is primarily determined by the CSQ1 abundance, and in type I fibres, by the combined amounts of both CSQ1 and CSQ2.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CASQ1 elisa kitDiseases associated with CASQ1 elisa kit
 Products by Pathway  Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 Disease Name  Pubmed Publications
 Muscular Diseases Antibodies  >10 publications with CASQ1 and Muscular Diseases
 Exophthalmos Antibodies  >4 publications with CASQ1 and Exophthalmos
 Necrosis Antibodies  >2 publications with CASQ1 and Necrosis
 Neoplasms Antibodies  >2 publications with CASQ1 and Neoplasms
 Retinal Degeneration Antibodies  >1 publications with CASQ1 and Retinal Degeneration
 Pain Antibodies  >1 publications with CASQ1 and Pain
 Drug Toxicity Antibodies  >1 publications with CASQ1 and Drug Toxicity
 Anemia Antibodies  >1 publications with CASQ1 and Anemia
 Dyslipidemias Antibodies  >1 publications with CASQ1 and Dyslipidemias
 Eyelid Diseases Antibodies  >1 publications with CASQ1 and Eyelid Diseases
Organs/Tissues associated with CASQ1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >39 publications with CASQ1 and Muscle
 Eye Antibodies  >5 publications with CASQ1 and Eye
 Heart Antibodies  >5 publications with CASQ1 and Heart
 Brain Antibodies  >2 publications with CASQ1 and Brain
 Placenta Antibodies  >1 publications with CASQ1 and Placenta
 Liver Antibodies  >1 publications with CASQ1 and Liver
 Prostate Antibodies  >1 publications with CASQ1 and Prostate
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