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KIAA1524 elisa kit :: Human cancerous inhibitor of PP2A (CIP2A) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7205249
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

cancerous inhibitor of PP2A (CIP2A) (KIAA1524), ELISA Kit

★Popular Item★
 Also Known As   

Human cancerous inhibitor of PP2A (CIP2A) ELISA Kit

 Product Gene Name   

KIAA1524 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS7205249 COA    COA PDF
 OMIM    610643
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of CIP2A. No significant cross-reactivity or interference between CIP2A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CIP2A and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    0.5-10ng/mL
 Sensitivity    0.1ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of KIAA1524 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for KIAA1524 purchase    MBS7205249 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cancerous inhibitor of PP2A (CIP2A) (KIAA1524) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10ng/mL in biological research samples containing KIAA1524, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS7205249 kit is based on KIAA1524 antibody-KIAA1524 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KIAA1524 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KIAA1524. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for KIAA1524 elisa kit

   Intended Uses: This CIP2A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CIP2A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CIP2A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CIP2A antibody and an CIP2A-HRP conjugate. The assay sample and buffer are incubated together with CIP2A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CIP2A concentration since CIP2A from samples and CIP2A-HRP conjugate compete for the anti-CIP2A antibody binding site. Since the number of sites is limited, as more sites are occupied by CIP2A from the sample, fewer sites are left to bind CIP2A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CIP2A concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for KIAA1524 elisa kit    Cancer
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 Typical Standard Curve/Testing Data of KIAA1524 elisa kit    KIAA1524 elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS7205249. Click to request current manual
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NCBI/Uniprot data below describe general gene information for KIAA1524. It may not necessarily be applicable to this product.
 NCBI GI #    223459610
 NCBI GeneID    57650
 NCBI Accession #    AAI36372.1 [Other Products]
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 UniProt Secondary Accession #    Q6P4G6; Q8WVP8; Q96PI2; Q9H9C6; Q9P204; A1L4J7; B9EGC3 [Other Products]
 UniProt Related Accession #    Q8TCG1 [Other Products]
 Molecular Weight    84,579 Da
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 NCBI Official Full Name    KIAA1524
 NCBI Official Synonym Full Names    KIAA1524
 NCBI Official Symbol    KIAA1524 [Similar Products]
 NCBI Official Synonym Symbols   
p90; CIP2A
[Similar Products]
 NCBI Protein Information    protein CIP2A; p90 autoantigen; cancerous inhibitor of PP2A; cancerous inhibitor of protein phosphatase 2A
 UniProt Protein Name    Protein CIP2A
 UniProt Synonym Protein Names   
Cancerous inhibitor of PP2A; p90 autoantigen
 Protein Family    Protein
 UniProt Gene Name    KIAA1524 [Similar Products]
 UniProt Synonym Gene Names    CIP2A [Similar Products]
 UniProt Entry Name    CIP2A_HUMAN
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 UniProt Comments for KIAA1524    CIP2A: an oncoprotein that inhibits PP2A in human malignancies. Like the SV40 small t antigen, inhibits the phospho-serine/-threonine phosphatase PP2A. Promotes c-Myc protein stability by inhibiting the dephosphorylation of phospho-serine 62. Expressed at low levels in most of nonmalignant tissues but at high levels in human malignancies including head and neck squamous cell carcinoma (HNSCC), colon cancer, and liver cancer. Antibodies against CIP2A are present in sera from many patients with gastric or prostate cancer. Two alternatively spliced human isoforms have been described.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q13.13

Cellular Component: cytoplasm; integral to membrane; plasma membrane

Molecular Function: protein binding

Biological Process: spermatogenesis
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 Research Articles on KIAA1524    1. Downregulation of CIP2A attenuates migration and metastatic potential of cutaneous malignant melanoma cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with KIAA1524 elisa kitDiseases associated with KIAA1524 elisa kit
 Reference Product  PubMed Publications
 MYC elisa kit  >35 publications with KIAA1524 and MYC
 ETS1 elisa kit  >1 publications with KIAA1524 and ETS1
 Disease Name  Pubmed Publications
 Neoplasms, Experimental Antibodies  >9 publications with KIAA1524 and Neoplasms, Experimental
 Breast Neoplasms Antibodies  >8 publications with KIAA1524 and Breast Neoplasms
 Carcinoma, Hepatocellular Antibodies  >8 publications with KIAA1524 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >8 publications with KIAA1524 and Liver Neoplasms
 Liver Diseases Antibodies  >7 publications with KIAA1524 and Liver Diseases
 Neoplasm Metastasis Antibodies  >6 publications with KIAA1524 and Neoplasm Metastasis
 Lung Diseases Antibodies  >5 publications with KIAA1524 and Lung Diseases
 Cell Transformation, Neoplastic Antibodies  >4 publications with KIAA1524 and Cell Transformation, Neoplastic
 Neoplasm Invasiveness Antibodies  >4 publications with KIAA1524 and Neoplasm Invasiveness
 Hyperplasia Antibodies  >3 publications with KIAA1524 and Hyperplasia
Organs/Tissues associated with KIAA1524 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >8 publications with KIAA1524 and Liver
 Lung Antibodies  >7 publications with KIAA1524 and Lung
 Lymph Node Antibodies  >7 publications with KIAA1524 and Lymph Node
 Blood Antibodies  >4 publications with KIAA1524 and Blood
 Mouth Antibodies  >4 publications with KIAA1524 and Mouth
 Bone Marrow Antibodies  >2 publications with KIAA1524 and Bone Marrow
 Brain Antibodies  >2 publications with KIAA1524 and Brain
 Esophagus Antibodies  >1 publications with KIAA1524 and Esophagus
 Vascular Antibodies  >1 publications with KIAA1524 and Vascular
 Kidney Antibodies  >1 publications with KIAA1524 and Kidney
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