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CA7 elisa kit :: Human carbonic anhydrase VII ELISA Kit

Scan QR to view Datasheet Catalog #    MBS930609
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

carbonic anhydrase VII (CA7), ELISA Kit

★Popular Item★
 Also Known As   

Human Carbonic anhydrase 7, CA7 ELISA Kit

 Product Synonym Names    Human Carbonic anhydrase 7 (CA7) ELISA kit; CAVII; carbonic anhydrase 7; carbonic dehydratase VII; carbonic anhydrase VII
 Product Gene Name   

CA7 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS930609 Testing Data    Testing Data PDF
 OMIM    114770
 3D Structure    ModBase 3D Structure for P43166
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human CA7. No significant cross-reactivity or interference between human CA7 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    125 pg/ml -8000 pg/ml.
 Sensitivity    The minimum detectable dose of human CA7 is typically less than 31.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CA7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CA7 purchase    MBS930609 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the carbonic anhydrase VII (CA7) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 125 pg/ml -8000 pg/ml in biological research samples containing CA7, with an estimated sensitivity of The minimum detectable dose of human CA7 is typically less than 31.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS930609 kit is based on CA7 antibody-CA7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CA7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CA7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CA7 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CA7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CA7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CA7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CA7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of CA7 elisa kit    CA7 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS930609. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CA7. It may not necessarily be applicable to this product.
 NCBI GI #    62240989
 NCBI GeneID    766
 NCBI Accession #    NP_001014435.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001014435.1 [Other Products]
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 UniProt Primary Accession #    P43166 [Other Products]
 UniProt Secondary Accession #    Q541F0; Q86YU0 [Other Products]
 UniProt Related Accession #    P43166 [Other Products]
 Molecular Weight    29,658 Da
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 NCBI Official Full Name    carbonic anhydrase 7 isoform 2
 NCBI Official Synonym Full Names    carbonic anhydrase VII
 NCBI Official Symbol    CA7 [Similar Products]
 NCBI Official Synonym Symbols   
CAVII
[Similar Products]
 NCBI Protein Information    carbonic anhydrase 7; CA-VII; carbonic dehydratase VII; carbonate dehydratase VII
 UniProt Protein Name    Carbonic anhydrase 7
 UniProt Synonym Protein Names   
Carbonate dehydratase VII; Carbonic anhydrase VII; CA-VII
 Protein Family    Carbonic anhydrase
 UniProt Gene Name    CA7 [Similar Products]
 UniProt Synonym Gene Names    CA-VII [Similar Products]
 UniProt Entry Name    CAH7_HUMAN
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 NCBI Summary for CA7    Carbonic anhydrases are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. The cytosolic protein encoded by this gene is predominantly expressed in the salivary glands. Alternative splicing in the coding region results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CA7    CA7: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: cytosol

Molecular Function: carbonate dehydratase activity; zinc ion binding

Biological Process: bicarbonate transport; positive regulation of cellular pH reduction; positive regulation of synaptic transmission, GABAergic; one-carbon compound metabolic process
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 Research Articles on CA7    1. native hCA VII can protect cells from oxidative damage by preventing the apoptosis cascade and that cysteines play a leading role in this process
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CA7 elisa kitPathways associated with CA7 elisa kit
 Reference Product  PubMed Publications
 CALB1 elisa kit  >1 publications with CA7 and CALB1
 CALB2 elisa kit  >1 publications with CA7 and CALB2
 Products by Pathway  Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Nitrogen Metabolism Pathway antibodies  Nitrogen Metabolism Pathway Diagram
 Nitrogen Metabolism Pathway antibodies  Nitrogen Metabolism Pathway Diagram
 Reversible Hydration Of Carbon Dioxide Pathway antibodies  Reversible Hydration Of Carbon Dioxide Pathway Diagram
Diseases associated with CA7 elisa kitOrgans/Tissues associated with CA7 elisa kit
 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >3 publications with CA7 and Disease Models, Animal
 Necrosis Antibodies  >1 publications with CA7 and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with CA7 and Brain
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