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CERK elisa kit :: Human ceramide kinase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS919327
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ceramide kinase (CERK), ELISA Kit

★Popular Item★
 Also Known As   

Human Ceramide kinase, CERK ELISA Kit

 Product Synonym Names    Human Ceramide kinase (CERK) ELISA kit; RP6-59H18.1; DKFZp434E0211; FLJ21430; FLJ23239; KIAA1646; LK4; MGC131878; dA59H18.2; dA59H18.3; hCERK; lipid kinase LK4; ceramide kinase
 Product Gene Name   

CERK elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS919327 Testing Data    Testing Data PDF
 OMIM    610307
 3D Structure    ModBase 3D Structure for Q8TCT0
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human CERK. No significant cross-reactivity or interference between human CERK and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates
 Assay Type    Quantitative Sandwich
 Detection Range    18.75 pg/ml -1200 pg/ml
 Sensitivity    4.67 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CERK elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CERK purchase    MBS919327 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ceramide kinase (CERK) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 18.75 pg/ml -1200 pg/ml in biological research samples containing CERK, with an estimated sensitivity of 4.67 pg/ml. The ELISA analytical biochemical technique of the MBS919327 kit is based on CERK antibody-CERK antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CERK antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CERK. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CERK elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CERK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CERK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CERK is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CERK bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of CERK elisa kit    CERK elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS919327. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CERK. It may not necessarily be applicable to this product.
 NCBI GI #    20336726
 NCBI GeneID    64781
 NCBI Accession #    NP_073603.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_022766.5 [Other Products]
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 UniProt Primary Accession #    Q8TCT0 [Other Products]
 UniProt Secondary Accession #    Q9BYB3; Q9UGE5; A0JNT4; A8K611 [Other Products]
 UniProt Related Accession #    Q8TCT0 [Other Products]
 Molecular Weight    59,977 Da
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 NCBI Official Full Name    ceramide kinase
 NCBI Official Synonym Full Names    ceramide kinase
 NCBI Official Symbol    CERK [Similar Products]
 NCBI Official Synonym Symbols   
LK4; hCERK; dA59H18.2; dA59H18.3
[Similar Products]
 NCBI Protein Information    ceramide kinase; lipid kinase 4; lipid kinase LK4; acylsphingosine kinase
 UniProt Protein Name    Ceramide kinase
 UniProt Synonym Protein Names   
Acylsphingosine kinase; Lipid kinase 4; LK4
 Protein Family    Ceramide kinase
 UniProt Gene Name    CERK [Similar Products]
 UniProt Synonym Gene Names    KIAA1646; hCERK; LK4 [Similar Products]
 UniProt Entry Name    CERK1_HUMAN
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 NCBI Summary for CERK    CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al., 2006 [PubMed 16488390]).[supplied by OMIM, Mar 2008]
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 UniProt Comments for CERK    CERK: Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides.

Protein type: EC 2.7.1.138; Kinase, lipid; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: mitochondrion; plasma membrane; integral to membrane

Molecular Function: ceramide kinase activity; magnesium ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: sphingolipid metabolic process; protein kinase C activation; glycosphingolipid metabolic process; ceramide metabolic process; lipid phosphorylation; transmembrane transport
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 Research Articles on CERK    1. Data suggest that inhibition of ceramide kinase (CerK) [by specific gene silencing or pharmacological inhibition] drastically reduced cell proliferation in a neuroblastoma cell line.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CERK elisa kitDiseases associated with CERK elisa kit
 Products by Pathway  Pathway Diagram
 Glycosphingolipid Metabolism Pathway antibodies  Glycosphingolipid Metabolism Pathway Diagram
 Integrated Breast Cancer Pathway antibodies  Integrated Breast Cancer Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >12 publications with CERK and Inflammation
 Neoplasms Antibodies  >7 publications with CERK and Neoplasms
 Cardiovascular Diseases Antibodies  >4 publications with CERK and Cardiovascular Diseases
 Vascular Diseases Antibodies  >4 publications with CERK and Vascular Diseases
 Necrosis Antibodies  >4 publications with CERK and Necrosis
 Brain Diseases Antibodies  >4 publications with CERK and Brain Diseases
 Lung Diseases Antibodies  >3 publications with CERK and Lung Diseases
 Cerebrovascular Disorders Antibodies  >3 publications with CERK and Cerebrovascular Disorders
 Eye Diseases Antibodies  >2 publications with CERK and Eye Diseases
 Breast Neoplasms Antibodies  >1 publications with CERK and Breast Neoplasms
Organs/Tissues associated with CERK elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >13 publications with CERK and Blood
 Bone Antibodies  >5 publications with CERK and Bone
 Vascular Antibodies  >4 publications with CERK and Vascular
 Bone Marrow Antibodies  >4 publications with CERK and Bone Marrow
 Liver Antibodies  >3 publications with CERK and Liver
 Spleen Antibodies  >3 publications with CERK and Spleen
 Kidney Antibodies  >2 publications with CERK and Kidney
 Muscle Antibodies  >2 publications with CERK and Muscle
 Brain Antibodies  >2 publications with CERK and Brain
 Skin Antibodies  >1 publications with CERK and Skin
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