Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow CCL20 rightarrow Human CCL20  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • ABLIM1 Antibody
 • immunoglobulin-like domain containing receptor 2 (ILDR2) ELISA Kit
 • Acid Sphingomyelinase (ASM) Recombinant Protein
 • IGF-BP2 Recombinant Protein
 • M-CSF Receptor (Phospho-Tyr809) Antibody
 • Interleukin 35 (IL35) ELISA Kit
 • glucokinase (hexokinase 4) (GCK) ELISA Kit
 • cytochrome P450, CYP450 ELISA Kit
 • Adrenergic Receptor, beta 1 (Adrb1) Antibody
 • Claudin-20 (CLDN20) ELISA Kit
 • WWP2 Recombinant Protein
 • N-methylpurine-DNA glycosylase (MPG) Antibody
 • AAA ELISA Kit
 • Fish Serum (cell culture grade) Reagent
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

CCL20 elisa kit :: Human chemokine (C-C motif) ligand 20 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS701495
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $625 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,905 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

chemokine (C-C motif) ligand 20 (CCL20), ELISA Kit

★Popular Item★
 Also Known As   

Human Macrophage Inflammatory Protein-3alpha, MIP-3alpha ELISA Kit

 Product Synonym Names    Human Macrophage Inflammatory Protein-3alpha; MIP-3alpha ELISA kit; CKb4; LARC; MIP-3a; MIP3A; SCYA20; ST38; exodus-1; macrophage inflammatory protein 3 alpha; small inducible cytokine subfamily A (Cys-Cys); member 20; chemokine (C-C motif) ligand 20
 Product Gene Name   

CCL20 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS701495 Testing Data    Testing Data PDF
 OMIM    601960
 3D Structure    ModBase 3D Structure for P78556
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of Human CCL20. No significant cross-reactivity or interference between Human CCL20 and analogues was observed.
Section Bar
 Samples    Serum, plasma, cell culture supernates
 Assay Type    Sandwich
 Detection Range    15.6 pg/ml-1000 pg/ml
 Sensitivity    3.9 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Immunity
 Protein Biological Process 2    chemokine
 Protein Biological Process 3    Chemotaxis
Section Bar
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CCL20 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CCL20 purchase    MBS701495 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the chemokine (C-C motif) ligand 20 (CCL20) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 pg/ml-1000 pg/ml in biological research samples containing CCL20, with an estimated sensitivity of 3.9 pg/ml. The ELISA analytical biochemical technique of the MBS701495 kit is based on CCL20 antibody-CCL20 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CCL20 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CCL20. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for CCL20 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MIP-3alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MIP-3alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIP-3alpha is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIP-3alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of CCL20 elisa kit    CCL20 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS701495. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for CCL20. It may not necessarily be applicable to this product.
 NCBI GI #    194239688
 NCBI GeneID    6364
 NCBI Accession #    NP_001123518.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001130046.1 [Other Products]
Section Bar
 UniProt Primary Accession #    P78556 [Other Products]
 UniProt Secondary Accession #    Q53S51; Q99664 [Other Products]
 UniProt Related Accession #    P78556 [Other Products]
 Molecular Weight    10,762 Da
Table BarTOPTable Bar
 NCBI Official Full Name    C-C motif chemokine 20 isoform 2
 NCBI Official Synonym Full Names    chemokine (C-C motif) ligand 20
 NCBI Official Symbol    CCL20 [Similar Products]
 NCBI Official Synonym Symbols   
CKb4; LARC; ST38; MIP3A; MIP-3a; SCYA20; MIP-3-alpha
[Similar Products]
 NCBI Protein Information    C-C motif chemokine 20; CC chemokine LARC; beta chemokine exodus-1; small-inducible cytokine A20; macrophage inflammatory protein 3 alpha; liver and activation-regulated chemokine; small inducible cytokine subfamily A (Cys-Cys), member 20
 UniProt Protein Name    C-C motif chemokine 20
 UniProt Synonym Protein Names   
Beta-chemokine exodus-1; CC chemokine LARC; Liver and activation-regulated chemokine; Macrophage inflammatory protein 3 alpha; MIP-3-alpha; Small-inducible cytokine A20Cleaved into the following 3 chains:CCL20(1-67); CCL20(1-64); CCL20(2-70)
 Protein Family    C-C motif chemokine
 UniProt Gene Name    CCL20 [Similar Products]
 UniProt Synonym Gene Names    LARC; MIP3A; SCYA20; MIP-3-alpha [Similar Products]
 UniProt Entry Name    CCL20_HUMAN
Section Bar
 NCBI Summary for CCL20    This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
Section Bar
 UniProt Comments for CCL20    Function: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213.1 PublicationManual assertion based on experiment in:Ref.8
Table BarTOPTable Bar
 Research Articles on CCL20    1. CCL20 and CXCL8 synergize to promote colorectal metastatic progression by coordinated induction of EMT via PI3K/AKT-ERK1/2 signaling axis.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with CCL20 elisa kitPathways associated with CCL20 elisa kit
 Reference Product  PubMed Publications
 CCR6 elisa kit  >371 publications with CCL20 and CCR6
 CXCL10 elisa kit  >103 publications with CCL20 and CXCL10
 CXCL1 elisa kit  >93 publications with CCL20 and CXCL1
 CCL5 elisa kit  >91 publications with CCL20 and CCL5
 CXCL2 elisa kit  >73 publications with CCL20 and CXCL2
 CCR7 elisa kit  >58 publications with CCL20 and CCR7
 CXCL9 elisa kit  >54 publications with CCL20 and CXCL9
 CXCL3 elisa kit  >40 publications with CCL20 and CXCL3
 CCR5 elisa kit  >36 publications with CCL20 and CCR5
 CXCL11 elisa kit  >33 publications with CCL20 and CXCL11
 Products by Pathway  Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 EBV LMP1 Signaling Pathway antibodies  EBV LMP1 Signaling Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
Diseases associated with CCL20 elisa kitOrgans/Tissues associated with CCL20 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >357 publications with CCL20 and Inflammation
 Necrosis Antibodies  >204 publications with CCL20 and Necrosis
 Skin Diseases Antibodies  >100 publications with CCL20 and Skin Diseases
 Carcinoma Antibodies  >83 publications with CCL20 and Carcinoma
 Lung Diseases Antibodies  >72 publications with CCL20 and Lung Diseases
 Hypersensitivity Antibodies  >56 publications with CCL20 and Hypersensitivity
 Liver Diseases Antibodies  >41 publications with CCL20 and Liver Diseases
 Cardiovascular Diseases Antibodies  >34 publications with CCL20 and Cardiovascular Diseases
 Neoplasms, Experimental Antibodies  >33 publications with CCL20 and Neoplasms, Experimental
 Fibrosis Antibodies  >24 publications with CCL20 and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Thymus Antibodies  >11 publications with CCL20 and Thymus
 Mammary Gland Antibodies  >2 publications with CCL20 and Mammary Gland
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions