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CHIT1 elisa kit :: Human chitinase 1 (chitotriosidase) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS923372
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

chitinase 1 (chitotriosidase) (CHIT1), ELISA Kit

★Popular Item★
 Also Known As   

Human chitinase 1 (chitotriosidase) , CHIT1 ELISA Kit

 Product Synonym Names    Human chitinase 1 (chitotriosidase) (CHIT1) ELISA kit; CHI3; CHIT; FLJ00314; MGC125322; chitotriosidase; plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase; chitinase 1 (chitotriosidase)
 Product Gene Name   

CHIT1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS923372 Testing Data    Testing Data PDF
 OMIM    gene 614122
 3D Structure    ModBase 3D Structure for Q13231
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human CHIT1. No significant cross-reactivity or interference between human CHIT1 and analogues was observed.
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 Samples    serum, plasma, tissue homogenates, cell culture supernates.
 Assay Type    Sandwich (Quantitative)
 Detection Range    31.25 pg/ml-2000 pg/ml.
 Sensitivity    Typically less than 7.8 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CHIT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CHIT1 purchase    MBS923372 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the chitinase 1 (chitotriosidase) (CHIT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml-2000 pg/ml in biological research samples containing CHIT1, with an estimated sensitivity of Typically less than 7.8 pg/ml. The ELISA analytical biochemical technique of the MBS923372 kit is based on CHIT1 antibody-CHIT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CHIT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CHIT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as serum, plasma, tissue homogenates, cell culture supernates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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 Typical Testing Data/Standard Curve (for reference only) of CHIT1 elisa kit    CHIT1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS923372. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CHIT1. It may not necessarily be applicable to this product.
 NCBI GI #    388890228
 NCBI GeneID    1118
 NCBI Accession #    NP_001243054.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001256125.1 [Other Products]
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 UniProt Primary Accession #    Q13231 [Other Products]
 UniProt Secondary Accession #    Q0VGG5; Q0VGG6; Q3ZAR1; Q6ISC2; Q9H3V8; Q9UDJ8; B3KNW6; J3KN09 [Other Products]
 UniProt Related Accession #    Q13231 [Other Products]
 Molecular Weight    51,681 Da
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 NCBI Official Full Name    chitotriosidase-1 isoform 2
 NCBI Official Synonym Full Names    chitinase 1 (chitotriosidase)
 NCBI Official Symbol    CHIT1 [Similar Products]
 NCBI Official Synonym Symbols   
CHI3; CHIT; CHITD
[Similar Products]
 NCBI Protein Information    chitotriosidase-1; plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase
 UniProt Protein Name    Chitotriosidase-1
 UniProt Synonym Protein Names   
Chitinase-1
 Protein Family    Chitotriosidase
 UniProt Gene Name    CHIT1 [Similar Products]
 UniProt Entry Name    CHIT1_HUMAN
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 NCBI Summary for CHIT1    Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012]
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 UniProt Comments for CHIT1    CHIT1: Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity. Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; EC 3.2.1.14; Hydrolase; Carbohydrate Metabolism - amino sugar and nucleotide sugar

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: extracellular space; lysosome

Molecular Function: endochitinase activity; chitin binding; chitinase activity

Biological Process: polysaccharide catabolic process; response to bacterium; immune response; chitin catabolic process

Disease: Chitotriosidase Deficiency
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 Research Articles on CHIT1    1. c.1049_1072dup24 in CHIT1 presents an allele frequency of 0.22 in Spain.p.G102S in CHIT1 presents an allele frequency of 0.27 in Spain.Ser102 CHIT1 allele caused a significant reduction of chitotriosidase activity.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CHIT1 elisa kitDiseases associated with CHIT1 elisa kit
 Products by Pathway  Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Disease Name  Pubmed Publications
 Gaucher Disease Antibodies  >4 publications with CHIT1 and Gaucher Disease
 Neoplasms Antibodies  >3 publications with CHIT1 and Neoplasms
 Gastritis Antibodies  >1 publications with CHIT1 and Gastritis
 Leukemia, Myeloid Antibodies  >1 publications with CHIT1 and Leukemia, Myeloid
 Necrosis Antibodies  >1 publications with CHIT1 and Necrosis
 Cardiovascular Diseases Antibodies  >1 publications with CHIT1 and Cardiovascular Diseases
Organs/Tissues associated with CHIT1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >7 publications with CHIT1 and Blood
 Lung Antibodies  >3 publications with CHIT1 and Lung
 Heart Antibodies  >2 publications with CHIT1 and Heart
 Bone Marrow Antibodies  >1 publications with CHIT1 and Bone Marrow
 Brain Antibodies  >1 publications with CHIT1 and Brain
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