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CRHBP elisa kit :: Human corticotropin releasing hormone binding protein ELISA Kit

Scan QR to view Datasheet Catalog #    MBS920581
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

corticotropin releasing hormone binding protein (CRHBP), ELISA Kit

★Popular Item★
 Also Known As   

Human Corticotropin-releasing factor-binding protein, CRHBP ELISA Kit

 Product Synonym Names    Human Corticotropin-releasing factor-binding protein (CRHBP) ELISA kit; CRF-BP; CRFBP; corticotropin releasing hormone-binding protein; corticotropin releasing hormone binding protein
 Product Gene Name   

CRHBP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS920581 Testing Data    Testing Data PDF
 OMIM    122559
 3D Structure    ModBase 3D Structure for P24387
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human CRHBP. No significant cross-reactivity or interference between human CRHBP and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    15.6 pg/ml -1000 pg/ml.
 Sensitivity    The minimum detectable dose of human CRHBP is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CRHBP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CRHBP purchase    MBS920581 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the corticotropin releasing hormone binding protein (CRHBP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 pg/ml -1000 pg/ml in biological research samples containing CRHBP, with an estimated sensitivity of The minimum detectable dose of human CRHBP is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS920581 kit is based on CRHBP antibody-CRHBP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CRHBP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CRHBP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for CRHBP elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CRHBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CRHBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CRHBP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CRHBP bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of CRHBP elisa kit    CRHBP elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS920581. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CRHBP. It may not necessarily be applicable to this product.
 NCBI GI #    47080099
 NCBI GeneID    1393
 NCBI Accession #    NP_001873.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001882.3 [Other Products]
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 UniProt Primary Accession #    P24387 [Other Products]
 UniProt Secondary Accession #    Q53F32; Q6FHT5 [Other Products]
 UniProt Related Accession #    P24387 [Other Products]
 Molecular Weight    36,144 Da
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 NCBI Official Full Name    corticotropin-releasing factor-binding protein
 NCBI Official Synonym Full Names    corticotropin releasing hormone binding protein
 NCBI Official Symbol    CRHBP [Similar Products]
 NCBI Official Synonym Symbols   
CRFBP; CRF-BP
[Similar Products]
 NCBI Protein Information    corticotropin-releasing factor-binding protein; CRH-BP; CRF-binding protein; corticotropin releasing hormone-binding protein; corticotropin-releasing hormone-binding protein
 UniProt Protein Name    Corticotropin-releasing factor-binding protein
 UniProt Synonym Protein Names   
Corticotropin-releasing hormone-binding protein; CRH-BP
 Protein Family    Corticotropin-releasing factor-binding protein
 UniProt Gene Name    CRHBP [Similar Products]
 UniProt Synonym Gene Names    CRFBP; CRF-BP; CRF-binding protein; CRH-BP [Similar Products]
 UniProt Entry Name    CRHBP_HUMAN
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 NCBI Summary for CRHBP    Corticotropin-releasing hormone is a potent stimulator of synthesis and secretion of preopiomelanocortin-derived peptides. Although CRH concentrations in the human peripheral circulation are normally low, they increase throughout pregnancy and fall rapidly after parturition. Maternal plasma CRH probably originates from the placenta. Human plasma contains a CRH-binding protein which inactivates CRH and which may prevent inappropriate pituitary-adrenal stimulation in pregnancy. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CRHBP    CRHBP: Binds CRF and inactivates it. May prevent inappropriate pituitary-adrenal stimulation in pregnancy. Belongs to the CRF-binding protein family.

Protein type: Secreted, signal peptide; Secreted; Inhibitor

Chromosomal Location of Human Ortholog: 5q13.3

Cellular Component: multivesicular body; Golgi apparatus; extracellular space; microtubule; endoplasmic reticulum; dendrite; perikaryon; intracellular; nerve terminal; nucleus; secretory granule

Molecular Function: protein binding; corticotropin-releasing hormone binding; peptide binding

Biological Process: negative regulation of adrenocorticotropic hormone secretion; synaptic transmission, dopaminergic; regulated secretory pathway; learning and/or memory; hormone-mediated signaling; cellular response to stress; regulation of adrenocorticotropin hormone secretion; hormone metabolic process; female pregnancy; behavioral response to ethanol; signal transduction; inflammatory response
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 Research Articles on CRHBP    1. Significant main effects on children's cortisol reactivity were found for loci on CRHR1 and CRHBP.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CRHBP elisa kitPathways associated with CRHBP elisa kit
 Reference Product  PubMed Publications
 CRH elisa kit  >30 publications with CRHBP and CRH
 CRHR1 elisa kit  >17 publications with CRHBP and CRHR1
 CRHR2 elisa kit  >10 publications with CRHBP and CRHR2
 POMC elisa kit  >7 publications with CRHBP and POMC
 UCN elisa kit  >7 publications with CRHBP and UCN
 UCN2 elisa kit  >3 publications with CRHBP and UCN2
 Products by Pathway  Pathway Diagram
 Corticotropin-releasing Hormone Pathway antibodies  Corticotropin-releasing Hormone Pathway Diagram
Diseases associated with CRHBP elisa kitOrgans/Tissues associated with CRHBP elisa kit
 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >10 publications with CRHBP and Mental Disorders
 Depressive Disorder Antibodies  >5 publications with CRHBP and Depressive Disorder
 Pain Antibodies  >3 publications with CRHBP and Pain
 Inflammation Antibodies  >3 publications with CRHBP and Inflammation
 Premature Birth Antibodies  >2 publications with CRHBP and Premature Birth
 Necrosis Antibodies  >2 publications with CRHBP and Necrosis
 Disease Models, Animal Antibodies  >2 publications with CRHBP and Disease Models, Animal
 Hypertension Antibodies  >2 publications with CRHBP and Hypertension
 Kidney Diseases Antibodies  >1 publications with CRHBP and Kidney Diseases
 Nervous System Diseases Antibodies  >1 publications with CRHBP and Nervous System Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >9 publications with CRHBP and Brain
 Ovary Antibodies  >3 publications with CRHBP and Ovary
 Skin Antibodies  >3 publications with CRHBP and Skin
 Kidney Antibodies  >3 publications with CRHBP and Kidney
 Placenta Antibodies  >3 publications with CRHBP and Placenta
 Embryonic Tissue Antibodies  >2 publications with CRHBP and Embryonic Tissue
 Adrenal Gland Antibodies  >2 publications with CRHBP and Adrenal Gland
 Liver Antibodies  >2 publications with CRHBP and Liver
 Connective Tissue Antibodies  >1 publications with CRHBP and Connective Tissue
 Uterus Antibodies  >1 publications with CRHBP and Uterus
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