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CUL1 elisa kit :: Human cullin 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS921353
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

cullin 1 (CUL1), ELISA Kit

★Popular Item★
 Also Known As   

Human Cullin-1, CUL1 ELISA Kit

 Product Synonym Names    Human Cullin-1 (CUL1) ELISA kit; MGC149834; MGC149835; OTTHUMP00000202347; cullin 1
 Product Gene Name   

CUL1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS921353 Testing Data    Testing Data PDF
 OMIM    603134
 3D Structure    ModBase 3D Structure for Q13616
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human CUL 1. No significant cross-reactivity or interference between human CUL 1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates
 Detection Range    46.88 pg/ml -3000 pg/mL
 Sensitivity    <11.72 pg/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CUL1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CUL1 purchase    MBS921353 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cullin 1 (CUL1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 46.88 pg/ml -3000 pg/mL in biological research samples containing CUL1, with an estimated sensitivity of <11.72 pg/mL. The ELISA analytical biochemical technique of the MBS921353 kit is based on CUL1 antibody-CUL1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CUL1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CUL1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CUL1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CUL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CUL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CUL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CUL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of CUL1 elisa kit    CUL1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS921353. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CUL1. It may not necessarily be applicable to this product.
 NCBI GI #    32307161
 NCBI GeneID    8454
 NCBI Accession #    NP_003583.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_003592.2 [Other Products]
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 UniProt Primary Accession #    Q13616 [Other Products]
 UniProt Secondary Accession #    O60719; Q08AL6; Q8IYW1; D3DWG3 [Other Products]
 UniProt Related Accession #    Q13616 [Other Products]
 Molecular Weight    89,679 Da
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 NCBI Official Full Name    cullin-1
 NCBI Official Synonym Full Names    cullin 1
 NCBI Official Symbol    CUL1 [Similar Products]
 NCBI Protein Information    cullin-1; CUL-1
 UniProt Protein Name    Cullin-1
 Protein Family    Cullin
 UniProt Gene Name    CUL1 [Similar Products]
 UniProt Synonym Gene Names    CUL-1 [Similar Products]
 UniProt Entry Name    CUL1_HUMAN
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 UniProt Comments for CUL1    CUL1: a core scaffolding component of multiple cullin-RING-based SCF (SKP1- CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. The cullin subunit serves as a rigid scaffold of SCF complexes. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex depends on the neddylation of the cullin subunit. Interacts with multiple F-box proteins in SCF complexes, directing the ubiquitination of many substrates. These F-box proteins and associated substrates include: BTRC and FBXW11 bind CTNNB1 and participate in Wnt signaling; FBXW11 bind phosphorylated NFKBIA; BTRC binds NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2; SKP2 binds phosphorylated p27kip and is involved in regulation of G1/S transition; SKP2 binds ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1; FBXW7 binds cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1; FBXW2 binds GCM1; FBXO32 binds MYOD1; FBXO7 binds BIRC2 and DLGAP5; FBXO33 binds YBX1; and Cyclin F binds CP110. Interacts with CHEK2, mediating its ubiquitination and regulating its function. Belongs to the cullin family.

Protein type: Ubiquitin conjugating system; Cell cycle regulation

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: nucleoplasm; cullin-RING ubiquitin ligase complex; SCF ubiquitin ligase complex; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: protein monoubiquitination; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; circadian rhythm; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; Notch signaling pathway; viral reproduction; protein ubiquitination; negative regulation of cell proliferation; cell proliferation; organ morphogenesis; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle; G2/M transition of mitotic cell cycle; cell cycle arrest; G1/S transition of mitotic cell cycle
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 Research Articles on CUL1    1. Studies indicate that in SCFs, Rbx1 serves as the RING-containing enzyme, Cul1 is the Cullin scaffold, and Skp1 is an adaptor, which serves to link the beta-TrCP F-box substrate-specific factor to the rest of the ligase.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CUL1 elisa kitPathways associated with CUL1 elisa kit
 Reference Product  PubMed Publications
 SKP1 elisa kit  >224 publications with CUL1 and SKP1
 RBX1 elisa kit  >66 publications with CUL1 and RBX1
 SKP2 elisa kit  >55 publications with CUL1 and SKP2
 NEDD8 elisa kit  >52 publications with CUL1 and NEDD8
 CAND1 elisa kit  >27 publications with CUL1 and CAND1
 FBXW7 elisa kit  >23 publications with CUL1 and FBXW7
 CDC34 elisa kit  >20 publications with CUL1 and CDC34
 BTRC elisa kit  >8 publications with CUL1 and BTRC
 FBXO25 elisa kit  >4 publications with CUL1 and FBXO25
 FBXW2 elisa kit  >4 publications with CUL1 and FBXW2
 Products by Pathway  Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 Activation Of NF-kappaB In B Cells Pathway antibodies  Activation Of NF-kappaB In B Cells Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Canonical Wnt Signaling Pathway antibodies  Canonical Wnt Signaling Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Circadian Clock Pathway antibodies  Circadian Clock Pathway Diagram
Diseases associated with CUL1 elisa kitOrgans/Tissues associated with CUL1 elisa kit
 Disease Name  Pubmed Publications
 Skin Diseases Antibodies  >7 publications with CUL1 and Skin Diseases
 Breast Neoplasms Antibodies  >6 publications with CUL1 and Breast Neoplasms
 Pregnancy Complications Antibodies  >4 publications with CUL1 and Pregnancy Complications
 Fetal Diseases Antibodies  >3 publications with CUL1 and Fetal Diseases
 Hypertrophy Antibodies  >3 publications with CUL1 and Hypertrophy
 Necrosis Antibodies  >2 publications with CUL1 and Necrosis
 Embryo Loss Antibodies  >2 publications with CUL1 and Embryo Loss
 Leukemia, Myeloid Antibodies  >2 publications with CUL1 and Leukemia, Myeloid
 Arthritis, Rheumatoid Antibodies  >2 publications with CUL1 and Arthritis, Rheumatoid
 Translocation, Genetic Antibodies  >2 publications with CUL1 and Translocation, Genetic
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >14 publications with CUL1 and Blood
 Embryonic Tissue Antibodies  >10 publications with CUL1 and Embryonic Tissue
 Nerve Antibodies  >9 publications with CUL1 and Nerve
 Brain Antibodies  >9 publications with CUL1 and Brain
 Kidney Antibodies  >8 publications with CUL1 and Kidney
 Muscle Antibodies  >8 publications with CUL1 and Muscle
 Heart Antibodies  >6 publications with CUL1 and Heart
 Eye Antibodies  >6 publications with CUL1 and Eye
 Liver Antibodies  >5 publications with CUL1 and Liver
 Vascular Antibodies  >5 publications with CUL1 and Vascular
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