Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow CDK5 rightarrow Human CDK5  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • ACC alpha (ACACA) Blocking Peptide
 • Transcription Factor AP-1 ELISA Kit
 • SOD1 Antibody
 • PSMA ELISA Kit
 • Macrosialin (Cd68) ELISA Kit
 • leukocyte common antigen (LCA) ELISA Kit
 • H-ACA ribonucleoprotein complex subunit 3 (Nop10) Recombinant Protein
 • Lipopolysaccharides (LPS) ELISA Kit
 • NF-kB p65 (RELA) Antibody
 • Alpha B Crystallin (CRYAB) ELISA Kit
 • Scl-70 Native Protein
 • prominin 1 (prom1) Antibody
 • Hepatitis B Core Antigen (HBcAg) (184 a.a.) 19kDa Recombinan... Recombinant Protein
 • TIM-1 Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

CDK5 elisa kit :: Human cyclin-dependent kinase 5 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700923
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

cyclin-dependent kinase 5 (CDK5), ELISA Kit

 Also Known As   

Human Cyclin Dependent Kinase 5, CDK5 ELISA Kit

 Product Synonym Names    Human Cyclin Dependent Kinase 5; CDK5 ELISA Kit; PSSALRE; protein kinase CDK5 splicing; cyclin-dependent kinase 5
 Product Gene Name   

CDK5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS700923 Testing Data    Testing Data PDF
 OMIM    123831
 3D Structure    ModBase 3D Structure for Q00535
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of Human CDK5. No significant cross-reactivity or interference between Human CDK5 and analogues was observed.
Section Bar
 Detection Range    1.56 ng/ml-100 ng/ml
 Sensitivity    0.39 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Apoptosis/Autophagy
 Protein Biological Process 3    Apoptosis
Section Bar
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CDK5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CDK5 purchase    MBS700923 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cyclin-dependent kinase 5 (CDK5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.56 ng/ml-100 ng/ml in biological research samples containing CDK5, with an estimated sensitivity of 0.39 ng/ml. The ELISA analytical biochemical technique of the MBS700923 kit is based on CDK5 antibody-CDK5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CDK5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CDK5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for CDK5 elisa kit

   Introduction: Cell division protein kinase 5 is an enzyme that in humans is encoded by the CDK5 gene.The protein encoded by this gene is part of the cyclin-dependent kinase family. Recently Cdk5 has emerged as an essential kinase in sensory pathways. Recent reports of Pareek et al. from NIH suggest its necessity in pain signaling. CDK5 is required for proper development of the brain and to be activated, CDK5 must associate with CDK5R1 or CDK5R2. Unlike other cyclin dependent kinases, CDK5 does not also require phosphorylation on the T loop so that binding with the activator is sufficient to activate the kinase. Cdk5 is involved in the processes of neuronal maturation and migration, phosphorylating the key intracellular adaptor of the reelin signaling chain. Experiments performed on mice lacking p35, a necessary activator of cdk5 in early brain development, showed that the normal layering of neurons was reversed in the cortex. This disrupted lamination again implicated cdk5 in neuronal migration and plasticity. Cdk5 is also involved in the regulation of synaptic vesicle exocytosis via phosphorylation of munc-18. Dysregulation of this enzyme has been implicated in several neurodegenerative diseases including Alzheimer's.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CDK5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CDK5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CDK5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CDK5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of CDK5 elisa kit    CDK5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS700923. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for CDK5. It may not necessarily be applicable to this product.
 NCBI GI #    256542306
 NCBI GeneID    1020
 NCBI Accession #    NP_001157882.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001164410.1 [Other Products]
Section Bar
 UniProt Primary Accession #    Q00535 [Other Products]
 UniProt Secondary Accession #    A1XKG3 [Other Products]
 UniProt Related Accession #    Q00535 [Other Products]
 Molecular Weight    33,304 Da
Table BarTOPTable Bar
 NCBI Official Full Name    cyclin-dependent kinase 5 isoform 2
 NCBI Official Synonym Full Names    cyclin-dependent kinase 5
 NCBI Official Symbol    CDK5 [Similar Products]
 NCBI Official Synonym Symbols   
PSSALRE
[Similar Products]
 NCBI Protein Information    cyclin-dependent kinase 5; TPKII catalytic subunit; protein kinase CDK5 splicing; cell division protein kinase 5; serine/threonine-protein kinase PSSALRE; tau protein kinase II catalytic subunit
 UniProt Protein Name    Cyclin-dependent kinase 5
 UniProt Synonym Protein Names   
Cell division protein kinase 5; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunit
 Protein Family    Cyclin-dependent kinase
 UniProt Gene Name    CDK5 [Similar Products]
 UniProt Synonym Gene Names    CDKN5; TPKII catalytic subunit [Similar Products]
 UniProt Entry Name    CDK5_HUMAN
Section Bar
 NCBI Summary for CDK5    This gene encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the protein encoded by this gene does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]
Section Bar
 UniProt Comments for CDK5    Function: Proline-directed serine/threonine-protein kinase essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. Interacts with D1 and D3-type G1 cyclins. Phosphorylates SRC, NOS3, VIM/vimentin, p35/CDK5R1, MEF2A, SIPA1L1, SH3GLB1, PXN, PAK1, MCAM/MUC18, SEPT5, SYN1, DNM1, AMPH, SYNJ1, CDK16, RAC1, RHOA, CDC42, TONEBP/NFAT5, MAPT/TAU, MAP1B, histone H1, p53/TP53, HDAC1, APEX1, PTK2/FAK1, huntingtin/HTT, ATM, MAP2, NEFH and NEFM. Regulates several neuronal development and physiological processes including neuronal survival, migration and differentiation, axonal and neurite growth, synaptogenesis, oligodendrocyte differentiation, synaptic plasticity and neurotransmission, by phosphorylating key proteins. Activated by interaction with CDK5R1 (p35) and CDK5R2 (p39), especially in post-mitotic neurons, and promotes CDK5R1 (p35) expression in an autostimulation loop. Phosphorylates many downstream substrates such as Rho and Ras family small GTPases (e.g. PAK1, RAC1, RHOA, CDC42) or microtubule-binding proteins (e.g. MAPT/TAU, MAP2, MAP1B), and modulates actin dynamics to regulate neurite growth and/or spine morphogenesis. Phosphorylates also exocytosis associated proteins such as MCAM/MUC18, SEPT5, SYN1, and CDK16/PCTAIRE1 as well as endocytosis associated proteins such as DNM1, AMPH and SYNJ1 at synaptic terminals. In the mature central nervous system (CNS), regulates neurotransmitter movements by phosphorylating substrates associated with neurotransmitter release and synapse plasticity; synaptic vesicle exocytosis, vesicles fusion with the presynaptic membrane, and endocytosis. Promotes cell survival by activating anti-apoptotic proteins BCL2 and STAT3, and negatively regulating of JNK3/MAPK10 activity. Phosphorylation of p53/TP53 in response to genotoxic and oxidative stresses enhances its stabilization by preventing ubiquitin ligase-mediated proteasomal degradation, and induces transactivation of p53/TP53 target genes, thus regulating apoptosis. Phosphorylation of p35/CDK5R1 enhances its stabilization by preventing calpain-mediated proteolysis producing p25/CDK5R1 and avoiding ubiquitin ligase-mediated proteasomal degradation. During aberrant cell-cycle activity and DNA damage, p25/CDK5 activity elicits cell-cycle activity and double-strand DNA breaks that precedes neuronal death by deregulating HDAC1. DNA damage triggered phosphorylation of huntingtin/HTT in nuclei of neurons protects neurons against polyglutamine expansion as well as DNA damage mediated toxicity. Phosphorylation of PXN reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Negative regulator of Wnt/beta-catenin signaling pathway. Activator of the GAIT (IFN-gamma-activated inhibitor of translation) pathway, which suppresses expression of a post-transcriptional regulon of proinflammatory genes in myeloid cells; phosphorylates the linker domain of glutamyl-prolyl tRNA synthetase (EPRS) in a IFN-gamma-dependent manner, the initial event in assembly of the GAIT complex. Phosphorylation of SH3GLB1 is required for autophagy induction in starved neurons. Phosphorylation of TONEBP/NFAT5 in response to osmotic stress mediates its rapid nuclear localization. MEF2 is inactivated by phosphorylation in nucleus in response to neurotoxin, thus leading to neuronal apoptosis. APEX1 AP-endodeoxyribonuclease is repressed by phosphorylation, resulting in accumulation of DNA damage and contributing to neuronal death. NOS3 phosphorylation down regulates NOS3-derived nitrite (NO) levels. SRC phosphorylation mediates its ubiquitin-dependent degradation and thus leads to cytoskeletal reorganization. May regulate endothelial cell migration and angiogenesis via the modulation of lamellipodia formation. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer in association with altered stability and subcellular distribution.21 PublicationsManual assertion based on experiment in:Ref.3

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Inhibited by 2-(1-ethyl-2-hydroxyethylamino)-6-benzylamino-9-isopropylpurine (roscovitine), 1-isopropyl-4-aminobenzyl-6-ether-linked benzimidazoles, resveratrol, AT-7519 and olomoucine. Activated by CDK5R1 (p35) and CDK5R2 (p39) during the development of the nervous system; degradation of CDK5R1 (p35) and CDK5R2 (p39) by proteasome result in down regulation of kinase activity, during this process, CDK5 phosphorylates p35 and induces its ubiquitination and subsequent degradation. Kinase activity is mainly determined by the amount of p35 available and subcellular location; reversible association to plasma membrane inhibits activity. Long-term inactivation as well as CDK5R1 (p25)-mediated hyperactivation of CDK5 triggers cell death. The pro-death activity of hyperactivated CDK5 is suppressed by membrane association of CDK5, via myristoylation of p35. Brain-derived neurotrophic factor, glial-derived neurotrophic factor, nerve growth factor (NGF), retinoic acid, laminin and neuregulin promote activity. Neurotoxicity enhances nuclear activity, thus leading to MEF2 phosphorylation and inhibition prior to apoptosis of cortical neurons. Repression by GSTP1 via p25/p35 translocation prevents neurodegeneration.5 PublicationsManual assertion based on experiment in:Ref.8
Table BarTOPTable Bar
 Research Articles on CDK5    1. CDK5 is essential for TGF-beta1-induced epithelial-mesenchymal transition and breast cancer progression.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with CDK5 elisa kitPathways associated with CDK5 elisa kit
 Reference Product  PubMed Publications
 MAPT elisa kit  >31 publications with CDK5 and MAPT
 PPP1R1B elisa kit  >14 publications with CDK5 and PPP1R1B
 NES elisa kit  >13 publications with CDK5 and NES
 CDK5R1 elisa kit  >13 publications with CDK5 and CDK5R1
 NDEL1 elisa kit  >12 publications with CDK5 and NDEL1
 CABLES1 elisa kit  >8 publications with CDK5 and CABLES1
 CDK5R2 elisa kit  >7 publications with CDK5 and CDK5R2
 CRMP1 elisa kit  >6 publications with CDK5 and CRMP1
 DPYSL2 elisa kit  >3 publications with CDK5 and DPYSL2
 Products by Pathway  Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 CRMPs In Sema3A Signaling Pathway antibodies  CRMPs In Sema3A Signaling Pathway Diagram
 Cocaine Addiction Pathway antibodies  Cocaine Addiction Pathway Diagram
 Cocaine Addiction Pathway antibodies  Cocaine Addiction Pathway Diagram
Diseases associated with CDK5 elisa kitOrgans/Tissues associated with CDK5 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >439 publications with CDK5 and Nervous System Diseases
 Brain Diseases Antibodies  >363 publications with CDK5 and Brain Diseases
 Mental Disorders Antibodies  >245 publications with CDK5 and Mental Disorders
 Nerve Degeneration Antibodies  >75 publications with CDK5 and Nerve Degeneration
 Movement Disorders Antibodies  >57 publications with CDK5 and Movement Disorders
 Inflammation Antibodies  >47 publications with CDK5 and Inflammation
 Cognition Disorders Antibodies  >41 publications with CDK5 and Cognition Disorders
 Pain Antibodies  >41 publications with CDK5 and Pain
 Memory Disorders Antibodies  >40 publications with CDK5 and Memory Disorders
 Learning Disorders Antibodies  >40 publications with CDK5 and Learning Disorders
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >734 publications with CDK5 and Brain
 Embryonic Tissue Antibodies  >65 publications with CDK5 and Embryonic Tissue
 Muscle Antibodies  >59 publications with CDK5 and Muscle
 Blood Antibodies  >54 publications with CDK5 and Blood
 Eye Antibodies  >44 publications with CDK5 and Eye
 Liver Antibodies  >21 publications with CDK5 and Liver
 Kidney Antibodies  >20 publications with CDK5 and Kidney
 Testis Antibodies  >18 publications with CDK5 and Testis
 Lung Antibodies  >16 publications with CDK5 and Lung
 Pancreas Antibodies  >16 publications with CDK5 and Pancreas
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions