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CYP11B2 elisa kit :: Human cytochrome P450, family 11, subfamily B, polypeptide 2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS933334
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

cytochrome P450, family 11, subfamily B, polypeptide 2 (CYP11B2), ELISA Kit

 Also Known As   

Human Cytochrome P450 11B2, mitochondrial, CYP11B2 ELISA Kit

 Product Synonym Names    Human Cytochrome P450 11B2; mitochondrial (CYP11B2) ELISA kit; ALDOS; CPN2; CYP11B; CYP11BL; P-450C18; P450C18; P450aldo; aldosterone synthase; cytochrome P450; subfamily XIB (steroid 11-beta-hydroxylase); polypeptide 2; mitochondrial cytochrome P450; family 11; ; cytochrome P450; family 11; subfamily B; polypeptide 2
 Product Gene Name   

CYP11B2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS933334 Testing Data    Testing Data PDF
 OMIM    610600
 3D Structure    ModBase 3D Structure for P19099
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human CYP11B2. No significant cross-reactivity or interference between Human CYP11B2 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    47 pg/ml-3000 pg/ml
 Sensitivity    11.7 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Biosynthesis/Metabolism
 Protein Biological Process 2    Lipogenesis and lipometabolism
 Protein Biological Process 3    Lipid metabolism
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CYP11B2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CYP11B2 purchase    MBS933334 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cytochrome P450, family 11, subfamily B, polypeptide 2 (CYP11B2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 47 pg/ml-3000 pg/ml in biological research samples containing CYP11B2, with an estimated sensitivity of 11.7 pg/ml. The ELISA analytical biochemical technique of the MBS933334 kit is based on CYP11B2 antibody-CYP11B2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP11B2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP11B2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CYP11B2 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYP11B2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYP11B2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP11B2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP11B2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of CYP11B2 elisa kit    CYP11B2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS933334. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CYP11B2. It may not necessarily be applicable to this product.
 NCBI GI #    119829183
 NCBI GeneID    1585
 NCBI Accession #    NP_000489.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_000498.3 [Other Products]
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 UniProt Primary Accession #    P19099 [Other Products]
 UniProt Secondary Accession #    Q16726; B0ZBE4 [Other Products]
 UniProt Related Accession #    P19099 [Other Products]
 Molecular Weight    57,560 Da
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 NCBI Official Full Name    cytochrome P450 11B2, mitochondrial
 NCBI Official Synonym Full Names    cytochrome P450, family 11, subfamily B, polypeptide 2
 NCBI Official Symbol    CYP11B2 [Similar Products]
 NCBI Official Synonym Symbols   
CPN2; ALDOS; CYP11B; CYP11BL; CYPXIB2; P450C18; P-450C18; P450aldo
[Similar Products]
 NCBI Protein Information    cytochrome P450 11B2, mitochondrial; cytochrome P-450C18; aldosterone synthase; cytochrome P-450Aldo; steroid 11-beta-monooxygenase; steroid 11-beta/18-hydroxylase; aldosterone-synthesizing enzyme; steroid 18-hydroxylase, aldosterone synthase, P450C18, P450aldo; mitochondrial cytochrome P450, family 11, subfamily B, polypeptide 2; cytochrome P450, subfamily XIB (steroid 11-beta-hydroxylase), polypeptide 2
 UniProt Protein Name    Cytochrome P450 11B2, mitochondrial
 UniProt Synonym Protein Names   
Aldosterone synthase (EC:1.14.15.4, EC:1.14.15.5); ALDOS; Aldosterone-synthesizing enzyme; CYPXIB2; Cytochrome P-450Aldo; Cytochrome P-450C18; Steroid 18-hydroxylase
 Protein Family    Cytochrome
 UniProt Gene Name    CYP11B2 [Similar Products]
 UniProt Synonym Gene Names    ALDOS [Similar Products]
 UniProt Entry Name    C11B2_HUMAN
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 NCBI Summary for CYP11B2    This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane. The enzyme has steroid 18-hydroxylase activity to synthesize aldosterone and 18-oxocortisol as well as steroid 11 beta-hydroxylase activity. Mutations in this gene cause corticosterone methyl oxidase deficiency. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CYP11B2    CYP11B2: Preferentially catalyzes the conversion of 11- deoxycorticosterone to aldosterone via corticosterone and 18- hydroxycorticosterone. Defects in CYP11B2 are the cause of corticosterone methyloxidase type 1 deficiency (CMO-1 deficiency); also known as aldosterone deficiency due to defect in 18- hydroxylase or aldosterone deficiency I. CMO-1 deficiency is an autosomal recessive disorder of aldosterone biosynthesis. There are two biochemically different forms of selective aldosterone deficiency be termed corticosterone methyloxidase (CMO) deficiency type 1 and type 2. In CMO-1 deficiency, aldosterone is undetectable in plasma, while its immediate precursor, 18- hydroxycorticosterone, is low or normal. Defects in CYP11B2 are the cause of corticosterone methyloxidase type 2 deficiency (CMO-2 deficiency). CMO-2 is an autosomal recessive disorder of aldosterone biosynthesis. In CMO-2 deficiency, aldosterone can be low or normal, but at the expense of increased secretion of 18- hydroxycorticosterone. Consequently, patients have a greatly increased ratio of 18-hydroxycorticosterone to aldosterone and a low ratio of corticosterone to 18-hydroxycorticosterone in serum. Defects in CYP11B2 are a cause of familial hyperaldosteronism type 1 (FH1). It is a disorder characterized by hypertension, variable hyperaldosteronism, and abnormal adrenal steroid production, including 18-oxocortisol and 18-hydroxycortisol. There is significant phenotypic heterogeneity, and some individuals never develop hypertension. The molecular defect causing hyperaldosteronism familial type 1 is an anti-Lepore-type fusion of the CYP11B1 and CYP11B2 genes. The hybrid gene has the promoting part of CYP11B1, ACTH-sensitive, and the coding part of CYP11B2. Belongs to the cytochrome P450 family.

Protein type: EC 1.14.15.5; EC 1.14.15.4; Oxidoreductase; Lipid Metabolism - C21-steroid hormone; Lipid Metabolism - androgen and estrogen; Mitochondrial

Chromosomal Location of Human Ortholog: 8q21-q22

Cellular Component: mitochondrion; mitochondrial inner membrane

Molecular Function: corticosterone 18-monooxygenase activity; steroid 11-beta-monooxygenase activity; iron ion binding; heme binding

Biological Process: steroid metabolic process; xenobiotic metabolic process; mineralocorticoid biosynthetic process; renal water homeostasis; C21-steroid hormone biosynthetic process; sodium ion homeostasis; aldosterone mediated regulation of blood volume; sterol metabolic process; aldosterone biosynthetic process; potassium ion homeostasis; cellular response to hormone stimulus

Disease: Corticosterone Methyloxidase Type Ii Deficiency; Corticosterone Methyloxidase Type I Deficiency
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 Research Articles on CYP11B2    1. Aldosteronomas are hypomethylated, and CYP11B2 is overexpressed and hypomethylated in these tumors.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CYP11B2 elisa kitPathways associated with CYP11B2 elisa kit
 Reference Product  PubMed Publications
 CYP11B1 elisa kit  >473 publications with CYP11B2 and CYP11B1
 POMC elisa kit  >96 publications with CYP11B2 and POMC
 NR5A1 elisa kit  >18 publications with CYP11B2 and NR5A1
 HSD11B2 elisa kit  >11 publications with CYP11B2 and HSD11B2
 HSD11B1 elisa kit  >4 publications with CYP11B2 and HSD11B1
 NPPA elisa kit  >1 publications with CYP11B2 and NPPA
 Products by Pathway  Pathway Diagram
 ACE Inhibitor Pathway antibodies  ACE Inhibitor Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 C21-Steroid Hormone Biosynthesis, Progesterone => Corticosterone/aldosterone Pathway antibodies  C21-Steroid Hormone Biosynthesis, Progesterone => Corticosterone/aldosterone Pathway Diagram
 C21-Steroid Hormone Biosynthesis, Progesterone => Corticosterone/aldosterone Pathway antibodies  C21-Steroid Hormone Biosynthesis, Progesterone => Corticosterone/aldosterone Pathway Diagram
 C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway antibodies  C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway Diagram
 C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway antibodies  C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway Diagram
 Cytochrome P450 - Arranged By Substrate Type Pathway antibodies  Cytochrome P450 - Arranged By Substrate Type Pathway Diagram
 Endogenous Sterols Pathway antibodies  Endogenous Sterols Pathway Diagram
 Glucocorticoid & Mineralcorticoid Metabolism Pathway antibodies  Glucocorticoid & Mineralcorticoid Metabolism Pathway Diagram
 Glucocorticoid Biosynthesis Pathway antibodies  Glucocorticoid Biosynthesis Pathway Diagram
Diseases associated with CYP11B2 elisa kitOrgans/Tissues associated with CYP11B2 elisa kit
 Disease Name  Pubmed Publications
 Hypertension Antibodies  >650 publications with CYP11B2 and Hypertension
 Hyperaldosteronism Antibodies  >208 publications with CYP11B2 and Hyperaldosteronism
 Heart Diseases Antibodies  >184 publications with CYP11B2 and Heart Diseases
 Heart Failure Antibodies  >107 publications with CYP11B2 and Heart Failure
 Fibrosis Antibodies  >97 publications with CYP11B2 and Fibrosis
 Kidney Diseases Antibodies  >96 publications with CYP11B2 and Kidney Diseases
 Hyperplasia Antibodies  >93 publications with CYP11B2 and Hyperplasia
 Hypertrophy Antibodies  >90 publications with CYP11B2 and Hypertrophy
 Inflammation Antibodies  >50 publications with CYP11B2 and Inflammation
 Cardiomegaly Antibodies  >49 publications with CYP11B2 and Cardiomegaly
 Organ/Tissue Name  Pubmed Publications
 Adrenal Gland Antibodies  >378 publications with CYP11B2 and Adrenal Gland
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