Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow CRLF1 rightarrow Human CRLF1  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • CRP ELISA Kit
 • Hemoglobin (Hb) Protein
 • ZFP-42-REX-1 Antibody
 • CD38 Antibody
 • Hydroxylysine (Hyl) ELISA Kit
 • Fibroblast growth factor 23 (FGF 23) ELISA Kit
 • Spermine (SM) ELISA Kit
 • HSD17B13 Antibody
 • Hemoglobin (HB) ELISA Kit
 • Amyloid Beta 42 (Abeta42) ELISA Kit
 • Urease Antibody
 • Interferon regulatory factor 4 (IRF4) Recombinant Protein
 • NFkB, p65 (NKIRAS2) Antibody
 • Hyaluronidase-1 (HYAL1) Recombinant Protein
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

CRLF1 elisa kit :: Human cytokine receptor-like factor 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS903158
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

cytokine receptor-like factor 1 (CRLF1), ELISA Kit

★Popular Item★
 Also Known As   

Human Cytokine receptor-like factor 1, CRLF1 ELISA Kit

 Product Synonym Names    Human Cytokine receptor-like factor 1 (CRLF1) ELISA kit; CISS; CISS1; CLF; CLF-1; NR6; zcytor5; class I cytokine receptor; cytokine type 1 receptor CRLP-1; cytokine receptor-like factor 1
 Product Gene Name   

CRLF1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS903158 Testing Data    Testing Data PDF
 OMIM    604237
 3D Structure    ModBase 3D Structure for O75462
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of Human CRLF1. No significant cross-reactivity or interference between Human CRLF1 and analogues was observed.
Section Bar
 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    0.156 ng/ml-10 ng/ml
 Sensitivity    0.039 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
Section Bar
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CRLF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CRLF1 purchase    MBS903158 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cytokine receptor-like factor 1 (CRLF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156 ng/ml-10 ng/ml in biological research samples containing CRLF1, with an estimated sensitivity of 0.039 ng/ml. The ELISA analytical biochemical technique of the MBS903158 kit is based on CRLF1 antibody-CRLF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CRLF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CRLF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Product Description specifically for CRLF1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CRLF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CRLF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CRLF1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CRLF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of CRLF1 elisa kit    CRLF1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS903158. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for CRLF1. It may not necessarily be applicable to this product.
 NCBI GI #    4758062
 NCBI GeneID    9244
 NCBI Accession #    NP_004741.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_004750.4 [Other Products]
Section Bar
 UniProt Primary Accession #    O75462 [Other Products]
 UniProt Secondary Accession #    Q9UHH5 [Other Products]
 UniProt Related Accession #    O75462 [Other Products]
 Molecular Weight    46,302 Da
Table BarTOPTable Bar
 NCBI Official Full Name    cytokine receptor-like factor 1
 NCBI Official Synonym Full Names    cytokine receptor-like factor 1
 NCBI Official Symbol    CRLF1 [Similar Products]
 NCBI Official Synonym Symbols   
CLF; NR6; CISS; CISS1; CLF-1; zcytor5
[Similar Products]
 NCBI Protein Information    cytokine receptor-like factor 1; cytokine-like factor 1; class I cytokine receptor; cytokine type 1 receptor CRLP-1
 UniProt Protein Name    Cytokine receptor-like factor 1
 UniProt Synonym Protein Names   
Cytokine-like factor 1; CLF-1; ZcytoR5
 Protein Family    Cytokine receptor-like factor
 UniProt Gene Name    CRLF1 [Similar Products]
 UniProt Synonym Gene Names    UNQ288/PRO327; CLF-1 [Similar Products]
 UniProt Entry Name    CRLF1_HUMAN
Section Bar
 NCBI Summary for CRLF1    This gene encodes a member of the cytokine type I receptor family. The protein forms a secreted complex with cardiotrophin-like cytokine factor 1 and acts on cells expressing ciliary neurotrophic factor receptors. The complex can promote survival of neuronal cells. Mutations in this gene result in Crisponi syndrome and cold-induced sweating syndrome. [provided by RefSeq, Oct 2009]
Section Bar
 UniProt Comments for CRLF1    CRLF1: Cytokine receptor subunit, possibly playing a regulatory role in the immune system and during fetal development. May be involved in nervous system development. Defects in CRLF1 are the cause of cold-induced sweating syndrome type 1 (CISS1). Cold-induced sweating syndrome (CISS) is an autosomal recessive disorder characterized by profuse sweating induced by cool surroundings (temperatures of 7 to 18 degrees Celsius). Additional abnormalities include a high- arched palate, nasal voice, depressed nasal bridge, inability to fully extend the elbows and kyphoscoliosis. Defects in CRLF1 are the cause of Crisponi syndrome (CRISPS). Crisponi syndrome is a rare autosomal recessive disorder characterized by congenital muscular contractions of facial muscles, with trismus in response to stimuli, dysmorphic features, bilateral camptodactyly, major feeding and respiratory difficulties, and access of hyperthermia leading to death in the first months of life. Belongs to the type I cytokine receptor family. Type 3 subfamily.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p12

Cellular Component: extracellular space

Molecular Function: protein binding; protein heterodimerization activity; cytokine binding; cytokine activity; receptor activity; ciliary neurotrophic factor receptor binding

Biological Process: ureteric bud development; positive regulation of cell proliferation; negative regulation of neuron apoptosis; positive regulation of tyrosine phosphorylation of Stat3 protein

Disease: Cold-induced Sweating Syndrome 1
Table BarTOPTable Bar
 Research Articles on CRLF1    1. Data indicate that CRLF1 serves its protective role by a cell autonomous mechanism that is independent of the gp130/JAK signaling pathway.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with CRLF1 elisa kitDiseases associated with CRLF1 elisa kit
 Reference Product  PubMed Publications
 CLCF1 elisa kit  >4 publications with CRLF1 and CLCF1
 CNTFR elisa kit  >2 publications with CRLF1 and CNTFR
 Disease Name  Pubmed Publications
 Crisponi syndrome Antibodies  >7 publications with CRLF1 and Crisponi syndrome
 COLD-INDUCED SWEATING SYNDROME 1 Antibodies  >7 publications with CRLF1 and COLD-INDUCED SWEATING SYNDROME 1
 Congenital Abnormalities Antibodies  >5 publications with CRLF1 and Congenital Abnormalities
 Skin Diseases Antibodies  >4 publications with CRLF1 and Skin Diseases
 Nervous System Diseases Antibodies  >3 publications with CRLF1 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >2 publications with CRLF1 and Cardiovascular Diseases
 Arrhythmias, Cardiac Antibodies  >1 publications with CRLF1 and Arrhythmias, Cardiac
 Respiratory System Abnormalities Antibodies  >1 publications with CRLF1 and Respiratory System Abnormalities
 Drug Toxicity Antibodies  >1 publications with CRLF1 and Drug Toxicity
 Inflammation Antibodies  >1 publications with CRLF1 and Inflammation
Organs/Tissues associated with CRLF1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >2 publications with CRLF1 and Skin
 Heart Antibodies  >1 publications with CRLF1 and Heart
 Embryonic Tissue Antibodies  >1 publications with CRLF1 and Embryonic Tissue
 Lung Antibodies  >1 publications with CRLF1 and Lung
 Brain Antibodies  >1 publications with CRLF1 and Brain
 Mouth Antibodies  >1 publications with CRLF1 and Mouth
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions