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DEFB103A elisa kit :: Human defensin, beta 103A ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700471
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

defensin, beta 103A (DEFB103A), ELISA Kit

★Popular Item★
 Also Known As   

Human beta-defensins 3 ELISA Kit

 Product Synonym Names    Human beta-defensins 3 ELISA Kit; DEFB103; DEFB3; HBD-3; HBD3; HBP-3; HBP3; beta-defensin 3; defensin; beta 3; defensin; beta 103A
 Product Gene Name   

DEFB103A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS700471 Testing Data    Testing Data PDF
 OMIM    606611
 3D Structure    ModBase 3D Structure for P81534
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human beta-defensins 3. No significant cross-reactivity or interference between human beta-defensins 3 and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, urine, tissue homogenates
 Assay Type    Quantitative Sandwich
 Detection Range    125 pg/ml-8000 pg/ml
 Sensitivity    Typically less than 31.25 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DEFB103A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DEFB103A purchase    MBS700471 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the defensin, beta 103A (DEFB103A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 125 pg/ml-8000 pg/ml in biological research samples containing DEFB103A, with an estimated sensitivity of Typically less than 31.25 pg/ml. The ELISA analytical biochemical technique of the MBS700471 kit is based on DEFB103A antibody-DEFB103A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DEFB103A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DEFB103A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, urine, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for DEFB103A elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for beta-defensins 3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any beta-defensins 3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for beta-defensins 3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of beta-defensins 3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of DEFB103A elisa kit    DEFB103A elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS700471. Click to request current manual
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NCBI/Uniprot data below describe general gene information for DEFB103A. It may not necessarily be applicable to this product.
 NCBI GI #    125656159
 NCBI GeneID    414325
 NCBI Accession #    NP_001075020.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001081551.3 [Other Products]
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 UniProt Primary Accession #    P81534 [Other Products]
 UniProt Secondary Accession #    Q8NFG6; Q9NPF6 [Other Products]
 UniProt Related Accession #    P81534 [Other Products]
 Molecular Weight    7,697 Da
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 NCBI Official Full Name    beta-defensin 103
 NCBI Official Synonym Full Names    defensin, beta 103A
 NCBI Official Symbol    DEFB103A [Similar Products]
 NCBI Official Synonym Symbols   
BD-3; HBD3; HBP3; DEFB3; HBP-3; hBD-3; DEFB-3; DEFB103
[Similar Products]
 NCBI Protein Information    beta-defensin 103; beta-defensin 3; defensin, beta 3; defensin, beta 103; defensin-like protein
 UniProt Protein Name    Beta-defensin 103
 UniProt Synonym Protein Names   
Beta-defensin 3; BD-3; DEFB-3; HBD3; hBD-3; Defensin, beta 103; Defensin-like protein
 Protein Family    Beta-defensin
 UniProt Gene Name    DEFB103A [Similar Products]
 UniProt Synonym Gene Names    BD3; DEFB103; DEFB3
DEFB103B; BD-3; DEFB-3; HBD3; hBD-3
[Similar Products]
 UniProt Entry Name    D103A_HUMAN
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 NCBI Summary for DEFB103A    Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 103, an antibiotic peptide which is induced by bacteria and interferon gamma, and which displays antimicrobial activity against S. aureus, S. pyogenes, P. aeruginosa, E. coli, and C. albicans. [provided by RefSeq, Oct 2014]
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 UniProt Comments for DEFB103A    Function: Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed.1 PublicationManual assertion based on experiment in:Ref.1
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 Research Articles on DEFB103A    1. these observations suggest that there may be an interracial difference in DEFB4/103A copy numbers between admixed populations and a relationship between DEFB1 single nucleotide polymorphisms and DEFB4/103A copy number variation
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with DEFB103A elisa kitPathways associated with DEFB103A elisa kit
 Reference Product  PubMed Publications
 CAMP elisa kit  >2 publications with DEFB103A and CAMP
 DEFB104A elisa kit  >2 publications with DEFB103A and DEFB104A
 Products by Pathway  Pathway Diagram
 Beta Defensins Pathway antibodies  Beta Defensins Pathway Diagram
 Defensins Pathway antibodies  Defensins Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
Diseases associated with DEFB103A elisa kit
 Disease Name  Pubmed Publications
 Metabolic Diseases Antibodies  >1 publications with DEFB103A and Metabolic Diseases
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