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DEFB104A elisa kit :: Human defensin, beta 104A ELISA Kit

Scan QR to view Datasheet Catalog #    MBS913953
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

defensin, beta 104A (DEFB104A), ELISA Kit

★Popular Item★
 Also Known As   

Human Beta-defensin 104, DEFB104A ELISA Kit

 Product Synonym Names    Human Beta-defensin 104 (DEFB104A) ELISA kit; BD-4; DEFB-4; DEFB104; DEFB4; MGC118942; MGC118944; MGC118945; hBD-4; defensin; beta 4; defensin; beta 104A
 Product Gene Name   

DEFB104A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS913953 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q8WTQ1
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human DEFB104A. No significant cross-reactivity or interference between human DEFB104A and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, tissue homogenates
 Assay Type    Sandwich
 Detection Range    31.25 pg/ml -2000 pg/ml.
 Sensitivity    The minimum detectable dose of human DEFB104A is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DEFB104A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DEFB104A purchase    MBS913953 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the defensin, beta 104A (DEFB104A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml -2000 pg/ml in biological research samples containing DEFB104A, with an estimated sensitivity of The minimum detectable dose of human DEFB104A is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS913953 kit is based on DEFB104A antibody-DEFB104A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DEFB104A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DEFB104A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for DEFB104A elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DEFB104A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DEFB104A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFB104A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFB104A bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of DEFB104A elisa kit    DEFB104A elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS913953. Click to request current manual
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NCBI/Uniprot data below describe general gene information for DEFB104A. It may not necessarily be applicable to this product.
 NCBI GI #    103485137
 NCBI GeneID    503618
 NCBI Accession #    NP_001035792.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001040702.1 [Other Products]
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 UniProt Primary Accession #    Q8WTQ1 [Other Products]
 UniProt Secondary Accession #    Q496I2; Q496I3; Q496I4 [Other Products]
 UniProt Related Accession #    Q8WTQ1 [Other Products]
 Molecular Weight    8,526 Da
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 NCBI Official Full Name    beta-defensin 104
 NCBI Official Synonym Full Names    defensin, beta 104B
 NCBI Official Symbol    DEFB104B [Similar Products]
 NCBI Official Synonym Symbols   
BD-4; hBD-4; DEFB-4
[Similar Products]
 NCBI Protein Information    beta-defensin 104; beta-defensin 4; defensin, beta 104
 UniProt Protein Name    Beta-defensin 104
 UniProt Synonym Protein Names   
Beta-defensin 4; BD-4; DEFB-4; hBD-4; Defensin, beta 104
 Protein Family    Beta-defensin
 UniProt Gene Name    DEFB104A [Similar Products]
 UniProt Synonym Gene Names    DEFB104; DEFB4
DEFB104B; BD-4; DEFB-4; hBD-4
[Similar Products]
 UniProt Entry Name    D104A_HUMAN
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 NCBI Summary for DEFB104A    Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Defensins are short, processed peptide molecules that are classified by structure into three groups: alpha-defensins, beta-defensins and theta-defensins. All beta-defensin genes are densely clustered in four to five syntenic chromosomal regions. Chromosome 8p23 contains at least two copies of the duplicated beta-defensin cluster. This duplication results in two identical copies of defensin, beta 104, DEFB104A and DEFB104B, in head-to-head orientation. This gene, DEFB104B, represents the more telomeric copy. [provided by RefSeq, Jul 2008]
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 UniProt Comments for DEFB104A    defensin, beta 4: Has antimicrobial activity. Synergistic effects with lysozyme and DEFB103. Belongs to the beta-defensin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: cell surface; extracellular region

Biological Process: defense response to bacterium; innate immune response
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 Research Articles on DEFB104A    1. Data suggest an activation of b-defensin-4 induction, in human knee meniscus by the osteoarthritis inflammatory process as a result of an endogenous antibiotic defense mechanism accompanied by an intrinsic effort of tissue remodeling.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with DEFB104A elisa kit
 Products by Pathway  Pathway Diagram
 Beta Defensins Pathway antibodies  Beta Defensins Pathway Diagram
 Defensins Pathway antibodies  Defensins Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
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