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DMP1 elisa kit :: Human dentin matrix acidic phosphoprotein 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS704117
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
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 Product Name   

dentin matrix acidic phosphoprotein 1 (DMP1), ELISA Kit

★Popular Item★
 Also Known As   

Human Dentin Matrix Protein 1, DMP1 ELISA Kit

 Product Synonym Names    Human Dentin Matrix Protein 1 (DMP1) ELISA kit; ARHP; ARHR; DMP-1; dentin matrix protein 1; dentin matrix acidic phosphoprotein 1
 Product Gene Name   

DMP1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS704117 Testing Data    Testing Data PDF
 OMIM    600980
 3D Structure    ModBase 3D Structure for Q13316
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human DMP1. No significant cross-reactivity or interference between Human DMP1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    0.312 ng/ml-20 ng/ml
 Sensitivity    0.078 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Biosynthesis/Metabolism
 Protein Biological Process 3    Biomineralization
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DMP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DMP1 purchase    MBS704117 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the dentin matrix acidic phosphoprotein 1 (DMP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312 ng/ml-20 ng/ml in biological research samples containing DMP1, with an estimated sensitivity of 0.078 ng/ml. The ELISA analytical biochemical technique of the MBS704117 kit is based on DMP1 antibody-DMP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DMP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DMP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for DMP1 elisa kit

   For samples:For the quantitative determination of human dentin matrix protein 1 (DMP1) concentrations in serum, plasma, tissue homogenates.

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DMP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DMP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of DMP1 elisa kit    DMP1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS704117. Click to request current manual
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NCBI/Uniprot data below describe general gene information for DMP1. It may not necessarily be applicable to this product.
 NCBI GI #    121256621
 NCBI GeneID    1758
 NCBI Accession #    NP_001073380.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001079911.2 [Other Products]
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 UniProt Primary Accession #    Q13316 [Other Products]
 UniProt Secondary Accession #    O43265; A1L4L3 [Other Products]
 UniProt Related Accession #    Q13316 [Other Products]
 Molecular Weight    55,782 Da
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 NCBI Official Full Name    dentin matrix acidic phosphoprotein 1 isoform 2
 NCBI Official Synonym Full Names    dentin matrix acidic phosphoprotein 1
 NCBI Official Symbol    DMP1 [Similar Products]
 NCBI Official Synonym Symbols   
ARHP; ARHR; DMP-1
[Similar Products]
 NCBI Protein Information    dentin matrix acidic phosphoprotein 1; dentin matrix protein 1
 UniProt Protein Name    Dentin matrix acidic phosphoprotein 1
 Protein Family    Dentin matrix acidic phosphoprotein
 UniProt Gene Name    DMP1 [Similar Products]
 UniProt Synonym Gene Names    DMP-1; Dentin matrix protein 1 [Similar Products]
 UniProt Entry Name    DMP1_HUMAN
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 NCBI Summary for DMP1    Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for DMP1    Function: May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite.1 PublicationManual assertion based on experiment in:Ref.6
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 Research Articles on DMP1    1. DMP-1 is a matrix marker expressed around osteocytes in human woven and lamellar bone and is useful in identifying osteosarcoma and other bone-forming tumours.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with DMP1 elisa kitPathways associated with DMP1 elisa kit
 Reference Product  PubMed Publications
 DSPP elisa kit  >107 publications with DMP1 and DSPP
 PHEX elisa kit  >66 publications with DMP1 and PHEX
 FGF23 elisa kit  >63 publications with DMP1 and FGF23
 MEPE elisa kit  >56 publications with DMP1 and MEPE
 IBSP elisa kit  >49 publications with DMP1 and IBSP
 ENPP1 elisa kit  >13 publications with DMP1 and ENPP1
 CD44 elisa kit  >8 publications with DMP1 and CD44
 AMELX elisa kit  >3 publications with DMP1 and AMELX
 Products by Pathway  Pathway Diagram
 ECM Proteoglycans Pathway antibodies  ECM Proteoglycans Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
Diseases associated with DMP1 elisa kitOrgans/Tissues associated with DMP1 elisa kit
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >45 publications with DMP1 and Kidney Diseases
 Hypophosphatemic Rickets, Autosomal Recessive, 1 Antibodies  >33 publications with DMP1 and Hypophosphatemic Rickets, Autosomal Recessive, 1
 Necrosis Antibodies  >12 publications with DMP1 and Necrosis
 Death Antibodies  >10 publications with DMP1 and Death
 Breast Neoplasms Antibodies  >9 publications with DMP1 and Breast Neoplasms
 Lung Neoplasms Antibodies  >7 publications with DMP1 and Lung Neoplasms
 Inflammation Antibodies  >7 publications with DMP1 and Inflammation
 Carcinoma Antibodies  >7 publications with DMP1 and Carcinoma
 Neoplasms, Experimental Antibodies  >7 publications with DMP1 and Neoplasms, Experimental
 Cell Transformation, Neoplastic Antibodies  >4 publications with DMP1 and Cell Transformation, Neoplastic
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with DMP1 and Bone
 Connective Tissue Antibodies  >4 publications with DMP1 and Connective Tissue
 Heart Antibodies  >3 publications with DMP1 and Heart
 Muscle Antibodies  >3 publications with DMP1 and Muscle
 Brain Antibodies  >3 publications with DMP1 and Brain
 Vascular Antibodies  >2 publications with DMP1 and Vascular
 Embryonic Tissue Antibodies  >2 publications with DMP1 and Embryonic Tissue
 Ear Antibodies  >1 publications with DMP1 and Ear
 Kidney Antibodies  >1 publications with DMP1 and Kidney
 Umbilical Cord Antibodies  >1 publications with DMP1 and Umbilical Cord
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