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DCD elisa kit :: Human dermcidin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700974
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

dermcidin (DCD), ELISA Kit

★Popular Item★
 Also Known As   

Human Proteolysis Inducing Factor/Dermcidin, PIF/DCD ELISA Kit

 Product Synonym Names    Human Proteolysis Inducing Factor/Dermcidin (PIF/DCD) ELISA Kit; AIDD; DCD-1; DSEP; HCAP; MGC71930; PIF; diffusible survival/evasion peptide; preproteolysin; proteolysis inducing factor; survival promoting peptide; dermcidin
 Product Gene Name   

DCD elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS700974 Testing Data    Testing Data PDF
 OMIM    606634
 3D Structure    ModBase 3D Structure for P81605
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human PIF/DCD. No significant cross-reactivity or interference between human PIF/DCD and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, urine, tissue homogenates
 Detection Range    0.625 ng/ml -40 ng/mL
 Sensitivity    <0.156 ng/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DCD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DCD purchase    MBS700974 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the dermcidin (DCD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625 ng/ml -40 ng/mL in biological research samples containing DCD, with an estimated sensitivity of <0.156 ng/mL. The ELISA analytical biochemical technique of the MBS700974 kit is based on DCD antibody-DCD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DCD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DCD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, urine, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for DCD elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PIF/DCD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PIF/DCD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIF/DCD is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIF/DCD bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of DCD elisa kit    DCD elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS700974. Click to request current manual
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NCBI/Uniprot data below describe general gene information for DCD. It may not necessarily be applicable to this product.
 NCBI GI #    16751921
 NCBI GeneID    117159
 NCBI Accession #    NP_444513.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_053283.3 [Other Products]
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 UniProt Primary Accession #    P81605 [Other Products]
 UniProt Secondary Accession #    P58461; Q53YJ2; A5JHP2; A5JHP3 [Other Products]
 UniProt Related Accession #    P81605 [Other Products]
 Molecular Weight    11,284 Da
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 NCBI Official Full Name    dermcidin isoform 1 preproprotein
 NCBI Official Synonym Full Names    dermcidin
 NCBI Official Symbol    DCD [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    dermcidin; preproteolysin; survival promoting peptide; proteolysis inducing factor; diffusible survival/evasion peptide
 UniProt Protein Name    Dermcidin
 UniProt Synonym Protein Names   
PreproteolysinCleaved into the following 2 chains:Survival-promoting peptide; DCD-1
 Protein Family    Dermcidin
 UniProt Gene Name    DCD [Similar Products]
 UniProt Synonym Gene Names    AIDD; DSEP [Similar Products]
 UniProt Entry Name    DCD_HUMAN
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 NCBI Summary for DCD    This gene encodes a secreted protein that is subsequently processed into mature peptides of distinct biological activities. The C-terminal peptide is constitutively expressed in sweat and has antibacterial and antifungal activities. The N-terminal peptide, also known as diffusible survival evasion peptide, promotes neural cell survival under conditions of severe oxidative stress. A glycosylated form of the N-terminal peptide may be associated with cachexia (muscle wasting) in cancer patients. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
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 UniProt Comments for DCD    Function: DCD-1 displays antimicrobial activity thereby limiting skin infection by potential pathogens in the first few hours after bacterial colonization. Highly effective against E.coli, E.faecalis, S.aureus and C.albicans. Optimal pH and salt concentration resemble the conditions in sweat. Also exhibits proteolytic activity.1 PublicationManual assertion based on experiment in:Ref.5

Catalytic activity: Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactor: Manganese. Required for survival-promoting peptide.
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 Research Articles on DCD    1. Crystal structure and functional mechanism of a human antimicrobial membrane channel
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with DCD elisa kitPathways associated with DCD elisa kit
 Reference Product  PubMed Publications
 UBC elisa kit  >2 publications with DCD and UBC
 CAMP elisa kit  >1 publications with DCD and CAMP
 Products by Pathway  Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Pyrimidine Deoxyribonuleotide Biosynthesis, CDP/CTP => DCDP/dCTP,dTDP/dTTP Pathway antibodies  Pyrimidine Deoxyribonuleotide Biosynthesis, CDP/CTP => DCDP/dCTP,dTDP/dTTP Pathway Diagram
 Pyrimidine Deoxyribonuleotide Biosynthesis, CDP/CTP => DCDP/dCTP,dTDP/dTTP Pathway antibodies  Pyrimidine Deoxyribonuleotide Biosynthesis, CDP/CTP => DCDP/dCTP,dTDP/dTTP Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram
Diseases associated with DCD elisa kitOrgans/Tissues associated with DCD elisa kit
 Disease Name  Pubmed Publications
 Ischemia Antibodies  >193 publications with DCD and Ischemia
 Cardiovascular Diseases Antibodies  >156 publications with DCD and Cardiovascular Diseases
 Liver Failure Antibodies  >50 publications with DCD and Liver Failure
 Kidney Diseases Antibodies  >43 publications with DCD and Kidney Diseases
 Necrosis Antibodies  >40 publications with DCD and Necrosis
 Inflammation Antibodies  >25 publications with DCD and Inflammation
 Renal Insufficiency Antibodies  >22 publications with DCD and Renal Insufficiency
 Memory Disorders Antibodies  >21 publications with DCD and Memory Disorders
 Pain Antibodies  >19 publications with DCD and Pain
 Fibrosis Antibodies  >18 publications with DCD and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Uncharacterized Tissue Antibodies  >1 publications with DCD and Uncharacterized Tissue
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