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DCT elisa kit :: Human dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS929342
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2) (DCT), ELISA Kit

 Also Known As   

Human L-dopachrome tautomerase, DCT ELISA Kit

 Product Synonym Names    Human L-dopachrome tautomerase (DCT) ELISA kit; TRP-2; TYRP2; dopachrome delta-isomerase; dopachrome tautomerase; tyrosinase-related protein 2; dopachrome tautomerase (dopachrome delta-isomerase; tyrosine-related protein 2)
 Product Gene Name   

DCT elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS929342 Testing Data    Testing Data PDF
 OMIM    191275
 3D Structure    ModBase 3D Structure for P40126
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human DCT. No significant cross-reactivity or interference between Human DCT and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    28 pg/ml-1800 pg/ml
 Sensitivity    7 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Biosynthesis/Metabolism
 Protein Biological Process 2    biogenic amine biosynthesis and metabolism
 Protein Biological Process 3    Melanin biosynthesis
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DCT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DCT purchase    MBS929342 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2) (DCT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 28 pg/ml-1800 pg/ml in biological research samples containing DCT, with an estimated sensitivity of 7 pg/ml. The ELISA analytical biochemical technique of the MBS929342 kit is based on DCT antibody-DCT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DCT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DCT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for DCT elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DCT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DCT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCT bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of DCT elisa kit    DCT elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS929342. Click to request current manual
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NCBI/Uniprot data below describe general gene information for DCT. It may not necessarily be applicable to this product.
 NCBI GI #    193788640
 NCBI GeneID    1638
 NCBI Accession #    NP_001123361.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001129889.1 [Other Products]
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 UniProt Primary Accession #    P40126 [Other Products]
 UniProt Secondary Accession #    Q09GT4 [Other Products]
 UniProt Related Accession #    P40126 [Other Products]
 Molecular Weight    59,145 Da
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 NCBI Official Full Name    L-dopachrome tautomerase isoform 2
 NCBI Official Synonym Full Names    dopachrome tautomerase
 NCBI Official Symbol    DCT [Similar Products]
 NCBI Official Synonym Symbols   
TRP-2; TYRP2
[Similar Products]
 NCBI Protein Information    L-dopachrome tautomerase; DT; TRP2; dopachrome delta-isomerase; tyrosine-related protein 2; L-dopachrome Delta-isomerase; tyrosinase-related protein 2; dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)
 UniProt Protein Name    L-dopachrome tautomerase
 UniProt Synonym Protein Names   
L-dopachrome Delta-isomerase; Tyrosinase-related protein 2; TRP-2; TRP2
 Protein Family    L-dopachrome tautomerase
 UniProt Gene Name    DCT [Similar Products]
 UniProt Synonym Gene Names    TYRP2; DCT; DT; TRP-2; TRP2 [Similar Products]
 UniProt Entry Name    TYRP2_HUMAN
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 UniProt Comments for DCT    DCT: Involved in regulating eumelanin and phaeomelanin levels. Belongs to the tyrosinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; EC 5.3.3.12; Membrane protein, integral; Amino Acid Metabolism - tyrosine; Isomerase

Chromosomal Location of Human Ortholog: 13q32

Cellular Component: melanosome membrane; integral to membrane; melanosome; cytosol

Molecular Function: copper ion binding; oxidoreductase activity; dopachrome isomerase activity

Biological Process: neuroblast division in the ventricular zone; positive regulation of neuroblast proliferation; epidermis development; pigmentation during development; melanin biosynthetic process from tyrosine; cell development
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 Research Articles on DCT    1. The results suggested that the miRNAs may be involved in MITF regulation of TYR, TYRP1 and TYRP2, which provides a new clue for understanding the role of miRNAs in melanocyte dysfunctional disease.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with DCT elisa kitDiseases associated with DCT elisa kit
 Products by Pathway  Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
 Tyrosine Metabolism Pathway antibodies  Tyrosine Metabolism Pathway Diagram
 Tyrosine Metabolism Pathway antibodies  Tyrosine Metabolism Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >128 publications with DCT and Neoplasms
 Cardiovascular Diseases Antibodies  >84 publications with DCT and Cardiovascular Diseases
 Hypertension Antibodies  >61 publications with DCT and Hypertension
 Nervous System Diseases Antibodies  >59 publications with DCT and Nervous System Diseases
 Melanoma Antibodies  >53 publications with DCT and Melanoma
 Kidney Diseases Antibodies  >42 publications with DCT and Kidney Diseases
 Carcinoma Antibodies  >42 publications with DCT and Carcinoma
 Heart Diseases Antibodies  >39 publications with DCT and Heart Diseases
 Disease Models, Animal Antibodies  >38 publications with DCT and Disease Models, Animal
 Skin Diseases Antibodies  >31 publications with DCT and Skin Diseases
Organs/Tissues associated with DCT elisa kit
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >245 publications with DCT and Kidney
 Eye Antibodies  >129 publications with DCT and Eye
 Skin Antibodies  >68 publications with DCT and Skin
 Heart Antibodies  >55 publications with DCT and Heart
 Brain Antibodies  >54 publications with DCT and Brain
 Bone Antibodies  >36 publications with DCT and Bone
 Lung Antibodies  >29 publications with DCT and Lung
 Embryonic Tissue Antibodies  >18 publications with DCT and Embryonic Tissue
 Intestine Antibodies  >12 publications with DCT and Intestine
 Connective Tissue Antibodies  >12 publications with DCT and Connective Tissue
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