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DNAH12 elisa kit :: Human dynein, axonemal, heavy chain 12 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS942482
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

dynein, axonemal, heavy chain 12 (DNAH12), ELISA Kit

★Popular Item★
 Also Known As   

Human Dynein heavy chain 12, axonemal, DNAH12 ELISA Kit

 Product Synonym Names    Human Dynein heavy chain 12; axonemal (DNAH12) ELISA kit; hCG_1779312; DHC3; DLP12; DNAH12L; DNAH7L; DNAHC12; DNAHC3; DNHD2; FLJ40427; FLJ44290; HL-19; HL19; hdhc3; Dynein; heavy chain-5; axonemal dynein heavy chain isotype3; dynein heavy chain 12; dynein hea; dynein; axonemal; heavy chain 12
 Product Gene Name   

DNAH12 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS942482 Testing Data    Testing Data PDF
 OMIM    603340
 3D Structure    ModBase 3D Structure for Q6ZR08
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human DNAH12. No significant cross-reactivity or interference between Human DNAH12 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    12.5 pg/ml-800 pg/ml
 Sensitivity    3.12 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Ubiquitin
 Protein Biological Process 3    Ubl conjugation pathway
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DNAH12 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DNAH12 purchase    MBS942482 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the dynein, axonemal, heavy chain 12 (DNAH12) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 12.5 pg/ml-800 pg/ml in biological research samples containing DNAH12, with an estimated sensitivity of 3.12 pg/ml. The ELISA analytical biochemical technique of the MBS942482 kit is based on DNAH12 antibody-DNAH12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DNAH12 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DNAH12. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for DNAH12 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DNAH12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DNAH12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNAH12 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNAH12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of DNAH12 elisa kit    DNAH12 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS942482. Click to request current manual
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NCBI/Uniprot data below describe general gene information for DNAH12. It may not necessarily be applicable to this product.
 NCBI GI #    89274173
 NCBI GeneID    201625
 NCBI Accession #    NP_940966.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_198564.3 [Other Products]
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 UniProt Primary Accession #    Q6ZR08 [Other Products]
 UniProt Secondary Accession #    Q6ZTR8; Q8N7R9; Q8WXK2; Q92816; A6NGI2 [Other Products]
 UniProt Related Accession #    Q6ZR08 [Other Products]
 Molecular Weight    356,942 Da
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 NCBI Official Full Name    dynein heavy chain 12, axonemal isoform 2
 NCBI Official Synonym Full Names    dynein, axonemal, heavy chain 12
 NCBI Official Symbol    DNAH12 [Similar Products]
 NCBI Official Synonym Symbols   
DHC3; HL19; DLP12; DNHD2; HDHC3; HL-19; DNAH7L; DNAHC3; DNAH12L; DNAHC12
[Similar Products]
 NCBI Protein Information    dynein heavy chain 12, axonemal; dynein, heavy chain-5; ciliary dynein heavy chain 12; axonemal beta dynein heavy chain 12; axonemal dynein heavy chain isotype3; dynein, axonemal, heavy polypeptide 12; dynein heavy chain domain-containing protein 2
 UniProt Protein Name    Dynein heavy chain 12, axonemal
 UniProt Synonym Protein Names   
Axonemal beta dynein heavy chain 12; Axonemal dynein heavy chain 12-like protein; Axonemal dynein heavy chain 7-like protein; Ciliary dynein heavy chain 12; Dynein heavy chain 7-like, axonemal; Dynein heavy chain domain-containing protein 2
 Protein Family    Dynein
 UniProt Gene Name    DNAH12 [Similar Products]
 UniProt Synonym Gene Names    DHC3; DLP12; DNAH12L; DNAH7L; DNAHC3; DNHD2; HDHC3; HL19 [Similar Products]
 UniProt Entry Name    DYH12_HUMAN
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 UniProt Comments for DNAH12    DNAHC12: Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Involved in sperm motility; implicated in sperm flagellar assembly. Belongs to the dynein heavy chain family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: dynein complex; microtubule; cytoplasm; cilium

Molecular Function: ATPase activity; microtubule motor activity; ATP binding

Biological Process: metabolic process; microtubule-based movement
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 Research Articles on DNAH12    1. Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator)
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with DNAH12 elisa kit
 Disease Name  Pubmed Publications
 Congenital Abnormalities Antibodies  >1 publications with DNAH12 and Congenital Abnormalities
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