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ENTPD4 elisa kit :: Human ectonucleoside triphosphate diphosphohydrolase 4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS906883
Typical Testing Data/Standard Curve (for reference only)
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 Product Name   

ectonucleoside triphosphate diphosphohydrolase 4 (ENTPD4), ELISA Kit

★Popular Item★
 Also Known As   

Human Ectonucleoside triphosphate diphosphohydrolase 4, ENTPD4 ELISA Kit

 Product Synonym Names    Human Ectonucleoside triphosphate diphosphohydrolase 4 (ENTPD4) ELISA kit; KIAA0392; LALP70; LAP70; LYSAL1; NTPDase-4; UDPase; apyrase; lysosomal; guanosine-diphosphatase like protein; lysosomal apyrase-like 1; ectonucleoside triphosphate diphosphohydrolase 4
 Product Gene Name   

ENTPD4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS906883 Testing Data    Testing Data PDF
 OMIM    607577
 3D Structure    ModBase 3D Structure for Q9Y227
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human ENTPD4. No significant cross-reactivity or interference between Human ENTPD4 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    15.6 pg/ml-1000 pg/ml
 Sensitivity    3.9 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ENTPD4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ENTPD4 purchase    MBS906883 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ectonucleoside triphosphate diphosphohydrolase 4 (ENTPD4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 pg/ml-1000 pg/ml in biological research samples containing ENTPD4, with an estimated sensitivity of 3.9 pg/ml. The ELISA analytical biochemical technique of the MBS906883 kit is based on ENTPD4 antibody-ENTPD4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ENTPD4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ENTPD4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ENTPD4 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ENTPD4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ENTPD4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ENTPD4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ENTPD4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ENTPD4 elisa kit    ENTPD4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS906883. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ENTPD4. It may not necessarily be applicable to this product.
 NCBI GI #    193083168
 NCBI GeneID    9583
 NCBI Accession #    NP_001122402.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001128930.2 [Other Products]
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 UniProt Primary Accession #    Q9Y227 [Other Products]
 UniProt Secondary Accession #    O15092; D3DSS3 [Other Products]
 UniProt Related Accession #    Q9Y227 [Other Products]
 Molecular Weight    70,255 Da
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 NCBI Official Full Name    ectonucleoside triphosphate diphosphohydrolase 4 isoform b
 NCBI Official Synonym Full Names    ectonucleoside triphosphate diphosphohydrolase 4
 NCBI Official Symbol    ENTPD4 [Similar Products]
 NCBI Official Synonym Symbols   
LAP70; LALP70; LYSAL1; UDPase; NTPDase-4
[Similar Products]
 NCBI Protein Information    ectonucleoside triphosphate diphosphohydrolase 4; golgi luminal UDPase; uridine-diphosphatase; lysosomal apyrase-like 1; guanosine-diphosphatase like protein; lysosomal apyrase-like protein of 70 kDa
 UniProt Protein Name    Ectonucleoside triphosphate diphosphohydrolase 4
 UniProt Synonym Protein Names   
Lysosomal apyrase-like protein of 70 kDa; Uridine-diphosphatase; UDPase
 Protein Family    Ectonucleoside triphosphate diphosphohydrolase
 UniProt Gene Name    ENTPD4 [Similar Products]
 UniProt Synonym Gene Names    KIAA0392; LALP70; LYSAL1; NTPDase 4; UDPase [Similar Products]
 UniProt Entry Name    ENTP4_HUMAN
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 NCBI Summary for ENTPD4    This gene encodes a member of the apyrase protein family. Apyrases are enzymes that catalyze the hydrolysis of nucleotide diphosphates and triphosphates in a calcium or magnesium-dependent manner. The encoded protein is an endo-apyrase and may play a role in salvaging nucleotides from lysosomes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and these isoforms may differ in divalent cation dependence and substrate specificity. [provided by RefSeq, Sep 2011]
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 UniProt Comments for ENTPD4    UDPase: an ectonucleoside triphosphate diphosphohydrolase. Hydrolyzes preferentially nucleoside 5'-diphosphates; nucleoside 5'-triphosphates are hydrolyzed only to a minor extent. The order of activity with different substrates is UDP >> GDP = CDP = TDP; AMP, ADP, ATP and UMP are not substrates. Two splice variant isoforms have been described. Preferred substrates for isoform 2 are CTP, UDP, CDP, GTP and GDP, while isoform 1 utilizes UTP and TTP

Protein type: Hydrolase; Nucleotide Metabolism - purine; Membrane protein, integral; EC; Membrane protein, multi-pass; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: intracellular membrane-bound organelle; integral to Golgi membrane; cytoplasmic vesicle

Molecular Function: uridine-diphosphatase activity

Biological Process: UDP catabolic process
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 Research Articles on ENTPD4    1. NTPDASE4 gene products cooperate with the adenovirus E4orf4 protein through PP2A-dependent and -independent mechanisms and contribute to induction of cell death.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ENTPD4 elisa kitOrgans/Tissues associated with ENTPD4 elisa kit
 Products by Pathway  Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1 publications with ENTPD4 and Brain
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