|
| Product Name
|
|
elastase 1, pancreatic (ELA1), ELISA Kit
|
| Also Known As
|
|
Human Chymotrypsin-like elastase family member 1, ELA1 ELISA Kit
|
| Product Synonym Names
|
|
Human Chymotrypsin-like elastase family member 1 (ELA1) ELISA kit; ELA1; elastase 1; pancreatic; elastase 1; pancreatic
|
| Product Gene Name
|
|
|
[Similar Products]
|
| Research Use Only
|
|
For Research Use Only. Not for use in diagnostic procedures.
|
 TOP
|
| Manual Insert
|
|
 
|
| MBS927335 Testing Data
|
|
|
|
| OMIM
|
|
130120
|
| 3D Structure
|
|
ModBase 3D Structure for Q9UNI1
|
|
| Species Reactivity
|
|
Human
|
|
|
| Specificity
|
|
This assay has high sensitivity and excellent specificity for detection of Human ELA1. No significant cross-reactivity or interference between Human ELA1 and analogues was observed.
|
|
|
| Samples
|
|
Serum, plasma, tissue homogenates, cell culture supernates and Cell lysates
|
| Assay Type
|
|
Sandwich
|
| Detection Range
|
|
0.625 ng/ml-40 ng/ml
|
| Sensitivity
|
|
0.156 ng/ml
|
| Intra-assay Precision
|
|
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
|
| Detection Wavelength
|
|
450 nm
|
| Sample Volume
|
|
50-100ul
|
|
|
| Preparation and Storage
|
|
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
|
| ISO Certification
|
|
Manufactured in an ISO 9001:2008 Certified Laboratory.
|
| Product Note
|
|
Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
|
| Other Notes
|
|
Small volumes of ELA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
|
| Searchable Terms for ELA1 purchase
|
|
MBS927335 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the elastase 1, pancreatic (ELA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625 ng/ml-40 ng/ml in biological research samples containing ELA1, with an estimated sensitivity of 0.156 ng/ml. The ELISA analytical biochemical technique of the MBS927335 kit is based on ELA1 antibody-ELA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ELA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ELA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell culture supernates and Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
|
|
TOP
|
|
Product Description specifically for ELA1 elisa kit
|
|
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ELA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ELA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ELA1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ELA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
|
|
|
|
| Typical Testing Data/Standard Curve (for reference only) of ELA1 elisa kit
|
|
|
|
TOP
|
|
| Sample Manual Insert of MBS927335 |
|
|
|
TOP
|
|
| NCBI/Uniprot data below describe general gene information for ELA1. It may not necessarily be applicable to this product. |
| NCBI GI #
|
|
58331209
|
| NCBI GeneID
|
|
1990
|
| NCBI Accession #
|
|
NP_001962.3
|
[Other Products]
|
| NCBI GenBank Nucleotide #
|
|
NM_001971.5
|
[Other Products]
|
|
|
| UniProt Primary Accession #
|
|
Q9UNI1
|
[Other Products]
|
| UniProt Secondary Accession #
|
|
Q5MLF0; Q6DJT0; Q6ISM6
|
[Other Products]
|
| UniProt Related Accession #
|
|
Q9UNI1
|
[Other Products]
|
| Molecular Weight
|
|
27,798 Da
|
|
TOP
|
| NCBI Official Full Name
|
|
chymotrypsin-like elastase family member 1
|
| NCBI Official Synonym Full Names
|
|
chymotrypsin-like elastase family, member 1
|
| NCBI Official Symbol
|
|
CELA1
|
[Similar Products]
|
| NCBI Official Synonym Symbols
|
|
ELA1
|
[Similar Products]
|
| NCBI Protein Information
|
|
chymotrypsin-like elastase family member 1; elastase-1; pancreatic elastase 1; elastase 1, pancreatic
|
| UniProt Protein Name
|
|
Chymotrypsin-like elastase family member 1
|
| UniProt Synonym Protein Names
|
|
Elastase-1; Pancreatic elastase 1
|
| Protein Family
|
|
Elongin
|
| UniProt Gene Name
|
|
CELA1
|
[Similar Products]
|
| UniProt Synonym Gene Names
|
|
ELA1
|
[Similar Products]
|
| UniProt Entry Name
|
|
CELA1_HUMAN
|
|
|
| NCBI Summary for ELA1
|
|
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, pancreatic elastase 1 is not expressed in the pancreas. To date, elastase 1 expression has only been detected in skin keratinocytes. Clinical literature that describes human elastase 1 activity in the pancreas or fecal material is actually referring to chymotrypsin-like elastase family, member 3B. [provided by RefSeq, May 2009]
|
|
|
| UniProt Comments for ELA1
|
|
CELA1: Acts upon elastin. Belongs to the peptidase S1 family. Elastase subfamily.
Protein type: EC 3.4.21.36; Secreted, signal peptide; Protease; Secreted
Chromosomal Location of Human Ortholog: 12q13
Cellular Component: extracellular region
Molecular Function: metal ion binding; serine-type endopeptidase activity
Biological Process: Wnt receptor signaling pathway; multicellular organism growth; regulation of cell differentiation; exocrine pancreas development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; inflammatory response; proteolysis; regulation of cell proliferation; post-embryonic development
|
|
TOP
|
|
| Research Articles on ELA1
|
|
1. Fecal elastase-1 is useful to identify pancreatic insufficiency. Steatorrhea is anticipated in non-operated patients only when FE-1 is below the limit for a confident measurement of our assay
|
|
TOP
|
| Precautions
|
|
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
|
| Disclaimer
|
|
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
|
|
TOP
|
| Products related to Human Chymotrypsin-like elastase family member 1, ELA1 ELISA Kit |
| Catalog # |
Product Name |
Catalog # |
Product Name |
| MBS082722 |
Goose Elastase 1, Pancreatic ELISA Kit |
MBS057403 |
Camel Elastase 1, Pancreatic ELISA Kit |
| MBS2006530 |
Polyclonal Antibody to Elastase 1, Pancreatic (ELA1) |
MBS9376198 |
Plant Elastase 1, Pancreatic (ELA1) ELISA Kit |
| MBS083855 |
Horse Elastase 1, Pancreatic ELISA Kit |
MBS087295 |
Donkey Elastase 1, Pancreatic ELISA Kit |
| MBS104591 |
Duck Elastase 1, Pancreatic ELISA Kit |
MBS2022131 |
Elastase 1, Pancreatic (ELA1) ELISA Kit |
| MBS085089 |
Hamster Elastase 1, Pancreatic ELISA Kit |
MBS091402 |
Fish Elastase 1, Pancreatic ELISA Kit |
|
|
|
| Diseases associated with ELA1 elisa kit |
|
|
|
|
|
TOP
|
ELISA Kit 
