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ELA1 elisa kit :: Human elastase 1, pancreatic ELISA Kit

Scan QR to view Datasheet Catalog #    MBS927335
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $510 +1 FREE 8GB USB
96-Strip-Wells  /  $725 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,565 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,800 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

elastase 1, pancreatic (ELA1), ELISA Kit

 Also Known As   

Human Chymotrypsin-like elastase family member 1, ELA1 ELISA Kit

 Product Synonym Names    Human Chymotrypsin-like elastase family member 1 (ELA1) ELISA kit; ELA1; elastase 1; pancreatic; elastase 1; pancreatic
 Product Gene Name   

ELA1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    PDF Manual
 MBS927335 Testing Data    Testing Data PDF
 OMIM    130120
 3D Structure    ModBase 3D Structure for Q9UNI1
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human ELA1. No significant cross-reactivity or interference between Human ELA1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell culture supernates and Cell lysates
 Assay Type    Sandwich
 Detection Range    0.625 ng/ml-40 ng/ml
 Sensitivity    0.156 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of ELA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ELA1 purchase    MBS927335 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the elastase 1, pancreatic (ELA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards render a theoretical kit detection range of 0.625 ng/ml-40 ng/ml in biological research samples containing ELA1, with an estimated sensitivity of 0.156 ng/ml. The ELISA analytical biochemical technique of the MBS927335 kit is based on ELA1 antibody-ELA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ELA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ELA1. Appropriate sample types include undiluted human body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell culture supernates and Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for ELA1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ELA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ELA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ELA1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ELA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ELA1 elisa kit    ELA1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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NCBI/Uniprot data below describe general gene information for ELA1. It may not necessarily be applicable to this product.
 NCBI GI #    58331209
 NCBI GeneID    1990
 NCBI Accession #    NP_001962.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_001971.5 [Other Products]
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 UniProt Primary Accession #    Q9UNI1 [Other Products]
 UniProt Secondary Accession #    Q5MLF0; Q6DJT0; Q6ISM6 [Other Products]
 UniProt Related Accession #    Q9UNI1 [Other Products]
 Molecular Weight    27,798 Da
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 NCBI Official Full Name    chymotrypsin-like elastase family member 1
 NCBI Official Synonym Full Names    chymotrypsin-like elastase family, member 1
 NCBI Official Symbol    CELA1 [Similar Products]
 NCBI Official Synonym Symbols   

ELA1

[Similar Products]
 NCBI Protein Information    chymotrypsin-like elastase family member 1; elastase-1; pancreatic elastase 1; elastase 1, pancreatic
 UniProt Protein Name    Chymotrypsin-like elastase family member 1
 UniProt Synonym Protein Names   

Elastase-1; Pancreatic elastase 1

 UniProt Gene Name    CELA1 [Similar Products]
 UniProt Synonym Gene Names    ELA1 [Similar Products]
 UniProt Entry Name    CELA1_HUMAN
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 NCBI Summary for ELA1    Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, pancreatic elastase 1 is not expressed in the pancreas. To date, elastase 1 expression has only been detected in skin keratinocytes. Clinical literature that describes human elastase 1 activity in the pancreas or fecal material is actually referring to chymotrypsin-like elastase family, member 3B. [provided by RefSeq, May 2009]
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 UniProt Comments for ELA1    CELA1: Acts upon elastin. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: EC 3.4.21.36; Secreted, signal peptide; Protease; Secreted

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: extracellular region

Molecular Function: metal ion binding; serine-type endopeptidase activity

Biological Process: Wnt receptor signaling pathway; multicellular organism growth; regulation of cell differentiation; exocrine pancreas development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; inflammatory response; proteolysis; regulation of cell proliferation; post-embryonic development
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 Research Articles on ELA1    1. Fecal elastase-1 is useful to identify pancreatic insufficiency. Steatorrhea is anticipated in non-operated patients only when FE-1 is below the limit for a confident measurement of our assay
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products related to Human Chymotrypsin-like elastase family member 1, ELA1 ELISA Kit
 Catalog #  Product Name  Catalog #  Product Name
 MBS085089  Hamster Elastase 1, Pancreatic ELISA Kit  MBS2533416  Human ELA1 (Elastase 1, Pancreatic) ELISA Kit
 MBS091402  Fish Elastase 1, Pancreatic ELISA Kit  MBS082722  Goose Elastase 1, Pancreatic ELISA Kit
 MBS104591  Duck Elastase 1, Pancreatic ELISA Kit  MBS051996  Sheep Elastase 1, Pancreatic ELISA Kit
 MBS049400  Porcine Elastase 1, Pancreatic ELISA Kit  MBS087295  Donkey Elastase 1, Pancreatic ELISA Kit
 MBS2008741  Biotin-Linked Antibody to Elastase 1, Pancreatic (ELA1)  MBS057403  Camel Elastase 1, Pancreatic ELISA Kit
Diseases associated with ELA1 elisa kit
 Disease Name  Pubmed Publications
 Endocrine System Diseases Antibodies  >1 publications with ELA1 and Endocrine System Diseases
 Neoplasms Antibodies  >1 publications with ELA1 and Neoplasms
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