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ELN elisa kit :: Human elastin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS704171
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

elastin (ELN), ELISA Kit

 Also Known As   

Human Elastin, ELN ELISA Kit

 Product Synonym Names    Human Elastin; ELN ELISA Kit; FLJ38671; FLJ43523; SVAS; WBS; WS; OTTHUMP00000211864; tropoelastin; elastin
 Product Gene Name   

ELN elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 MBS704171 Testing Data    Testing Data PDF
 OMIM    gene 185500
 3D Structure    ModBase 3D Structure for P15502
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human ELN. No significant cross-reactivity or interference between human ELN and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, urine, tissue homogenates, cell lysates.
 Assay Type    Sandwich
 Detection Range    3.12 pg/ml -200 pg/ml.
 Sensitivity    0.78 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of ELN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ELN purchase    MBS704171 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the elastin (ELN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.12 pg/ml -200 pg/ml in biological research samples containing ELN, with an estimated sensitivity of 0.78 pg/ml. The ELISA analytical biochemical technique of the MBS704171 kit is based on ELN antibody-ELN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ELN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ELN. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, urine, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for ELN elisa kit

   Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ELN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ELN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ELN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ELN bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ELN elisa kit    ELN elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS704171
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NCBI/Uniprot data below describe general gene information for ELN. It may not necessarily be applicable to this product.
 NCBI GI #    126352440
 NCBI GeneID    2006
 NCBI Accession #    NP_000492.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000501.3 [Other Products]
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 UniProt Primary Accession #    P15502 [Other Products]
 UniProt Secondary Accession #    O15336; O15337; Q14233; Q14234; Q14235; Q14238; Q6P0L4; Q6ZWJ6; Q75MU5; Q7Z316; B3KTS6 [Other Products]
 UniProt Related Accession #    P15502 [Other Products]
 Molecular Weight    68,469 Da
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 NCBI Official Full Name    elastin isoform a
 NCBI Official Synonym Full Names    elastin
 NCBI Official Symbol    ELN [Similar Products]
 NCBI Official Synonym Symbols   
WS; WBS; SVAS
[Similar Products]
 NCBI Protein Information    elastin; tropoelastin
 UniProt Protein Name    Elastin
 UniProt Synonym Protein Names   
Tropoelastin
 Protein Family    Elastin
 UniProt Gene Name    ELN [Similar Products]
 UniProt Entry Name    ELN_HUMAN
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 NCBI Summary for ELN    This gene encodes a protein that is one of the two components of elastic fibers. The encoded protein is rich in hydrophobic amino acids such as glycine and proline, which form mobile hydrophobic regions bounded by crosslinks between lysine residues. Deletions and mutations in this gene are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ELN    Function: Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle
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 Research Articles on ELN    1. Tropoelastin modulates TGF-beta1-induced expression of VEGF and CTGF in airway smooth muscle cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ELN elisa kitPathways associated with ELN elisa kit
 Reference Product  PubMed Publications
 LOXL1 elisa kit  >2 publications with ELN and LOXL1
 FBN1 elisa kit  >2 publications with ELN and FBN1
 Products by Pathway  Pathway Diagram
 Elastic Fibre Formation Pathway antibodies  Elastic Fibre Formation Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Protein Digestion And Absorption Pathway antibodies  Protein Digestion And Absorption Pathway Diagram
 Protein Digestion And Absorption Pathway antibodies  Protein Digestion And Absorption Pathway Diagram
Diseases associated with ELN elisa kitOrgans/Tissues associated with ELN elisa kit
 Disease Name  Pubmed Publications
 Heart Diseases Antibodies  >92 publications with ELN and Heart Diseases
 Aortic Stenosis, Supravalvular Antibodies  >70 publications with ELN and Aortic Stenosis, Supravalvular
 Nervous System Diseases Antibodies  >69 publications with ELN and Nervous System Diseases
 Williams Syndrome Antibodies  >62 publications with ELN and Williams Syndrome
 Hypertension Antibodies  >20 publications with ELN and Hypertension
 Disease Models, Animal Antibodies  >18 publications with ELN and Disease Models, Animal
 Cutis Laxa Antibodies  >10 publications with ELN and Cutis Laxa
 Heart Defects, Congenital Antibodies  >10 publications with ELN and Heart Defects, Congenital
 Lung Diseases Antibodies  >9 publications with ELN and Lung Diseases
 Inflammation Antibodies  >7 publications with ELN and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >43 publications with ELN and Vascular
 Muscle Antibodies  >30 publications with ELN and Muscle
 Heart Antibodies  >28 publications with ELN and Heart
 Brain Antibodies  >21 publications with ELN and Brain
 Connective Tissue Antibodies  >20 publications with ELN and Connective Tissue
 Skin Antibodies  >17 publications with ELN and Skin
 Lung Antibodies  >16 publications with ELN and Lung
 Intestine Antibodies  >12 publications with ELN and Intestine
 Eye Antibodies  >8 publications with ELN and Eye
 Bone Antibodies  >6 publications with ELN and Bone
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