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EPAS1 elisa kit :: Human endothelial PAS domain protein 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS702348
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
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 Product Name   

endothelial PAS domain protein 1 (EPAS1), ELISA Kit

★Popular Item★
 Also Known As   

Human hypoxia-inducible factor 2, HIF-2 ELISA Kit

 Product Synonym Names    Human hypoxia-inducible factor 2 (HIF-2) ELISA kit; ECYT4; HIF2A; HLF; MOP2; PASD2; bHLHe73; HIF-1alpha-like factor; basic-helix-loop-helix-PAS protein MOP2; hypoxia-inducible factor 2 alpha; member of PAS protein 2; endothelial PAS domain protein 1
 Product Gene Name   

EPAS1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS702348 Testing Data    Testing Data PDF
 OMIM    gene 611783
 3D Structure    ModBase 3D Structure for Q99814
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human HIF-2. No significant cross-reactivity or interference between human HIF-2 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates
 Assay Type    Quantitative Sandwich
 Detection Range    62.5 pg/ml -4000 pg/ml
 Sensitivity    Typically less than 15.6 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of EPAS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for EPAS1 purchase    MBS702348 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the endothelial PAS domain protein 1 (EPAS1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5 pg/ml -4000 pg/ml in biological research samples containing EPAS1, with an estimated sensitivity of Typically less than 15.6 pg/ml. The ELISA analytical biochemical technique of the MBS702348 kit is based on EPAS1 antibody-EPAS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EPAS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EPAS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for EPAS1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HIF-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HIF-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIF-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIF-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of EPAS1 elisa kit    EPAS1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS702348. Click to request current manual
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NCBI/Uniprot data below describe general gene information for EPAS1. It may not necessarily be applicable to this product.
 NCBI GI #    40254439
 NCBI GeneID    2034
 NCBI Accession #    NP_001421.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001430.4 [Other Products]
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 UniProt Primary Accession #    Q99814 [Other Products]
 UniProt Secondary Accession #    Q86VA2; Q99630 [Other Products]
 UniProt Related Accession #    Q99814 [Other Products]
 Molecular Weight    96,459 Da
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 NCBI Official Full Name    endothelial PAS domain-containing protein 1
 NCBI Official Synonym Full Names    endothelial PAS domain protein 1
 NCBI Official Symbol    EPAS1 [Similar Products]
 NCBI Official Synonym Symbols   
HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73
[Similar Products]
 NCBI Protein Information    endothelial PAS domain-containing protein 1; EPAS-1; HIF2-alpha; HIF-2-alpha; HIF-1alpha-like factor; HIF-1-alpha-like factor; member of PAS protein 2; PAS domain-containing protein 2; hypoxia-inducible factor 2 alpha; hypoxia-inducible factor 2-alpha; basic-helix-loop-helix-PAS protein MOP2; class E basic helix-loop-helix protein 73
 UniProt Protein Name    Endothelial PAS domain-containing protein 1
 UniProt Synonym Protein Names   
Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73; bHLHe73; HIF-1-alpha-like factor; HLF; Hypoxia-inducible factor 2-alpha; HIF-2-alpha; HIF2-alpha; Member of PAS protein 2; PAS domain-containing protein 2
 Protein Family    Endothelial PAS domain-containing protein
 UniProt Gene Name    EPAS1 [Similar Products]
 UniProt Synonym Gene Names    BHLHE73; HIF2A; MOP2; PASD2; EPAS-1; bHLHe73; HLF; HIF-2-alpha; HIF2-alpha [Similar Products]
 UniProt Entry Name    EPAS1_HUMAN
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 NCBI Summary for EPAS1    This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]
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 UniProt Comments for EPAS1    Function: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.
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 Research Articles on EPAS1    1. HIF-1 and HIF-2 specific gene activating functions respond differently to different stimuli.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with EPAS1 elisa kitPathways associated with EPAS1 elisa kit
 Reference Product  PubMed Publications
 EGLN1 elisa kit  >18 publications with EPAS1 and EGLN1
 VHL elisa kit  >14 publications with EPAS1 and VHL
 ARNT elisa kit  >10 publications with EPAS1 and ARNT
 VEGFA elisa kit  >7 publications with EPAS1 and VEGFA
 FLT1 elisa kit  >5 publications with EPAS1 and FLT1
 ARNT2 elisa kit  >2 publications with EPAS1 and ARNT2
 EGLN3 elisa kit  >1 publications with EPAS1 and EGLN3
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Cellular Response To Hypoxia Pathway antibodies  Cellular Response To Hypoxia Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 HIF-2-alpha Transcription Factor Network Pathway antibodies  HIF-2-alpha Transcription Factor Network Pathway Diagram
 Oxygen-dependent Asparagine Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway antibodies  Oxygen-dependent Asparagine Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway Diagram
 Oxygen-dependent Proline Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway antibodies  Oxygen-dependent Proline Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway antibodies  Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway Diagram
 Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway antibodies  Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway Diagram
Diseases associated with EPAS1 elisa kitOrgans/Tissues associated with EPAS1 elisa kit
 Disease Name  Pubmed Publications
 Carcinoma, Renal Cell Antibodies  >9 publications with EPAS1 and Carcinoma, Renal Cell
 Disease Models, Animal Antibodies  >8 publications with EPAS1 and Disease Models, Animal
 Kidney Diseases Antibodies  >7 publications with EPAS1 and Kidney Diseases
 Inflammation Antibodies  >6 publications with EPAS1 and Inflammation
 Necrosis Antibodies  >6 publications with EPAS1 and Necrosis
 Lung Diseases Antibodies  >4 publications with EPAS1 and Lung Diseases
 Hypertrophy Antibodies  >4 publications with EPAS1 and Hypertrophy
 Carcinoma, Hepatocellular Antibodies  >4 publications with EPAS1 and Carcinoma, Hepatocellular
 Liver Diseases Antibodies  >3 publications with EPAS1 and Liver Diseases
 Liver Neoplasms Antibodies  >3 publications with EPAS1 and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with EPAS1 and Bone
 Connective Tissue Antibodies  >4 publications with EPAS1 and Connective Tissue
 Heart Antibodies  >3 publications with EPAS1 and Heart
 Muscle Antibodies  >3 publications with EPAS1 and Muscle
 Brain Antibodies  >3 publications with EPAS1 and Brain
 Vascular Antibodies  >2 publications with EPAS1 and Vascular
 Embryonic Tissue Antibodies  >2 publications with EPAS1 and Embryonic Tissue
 Ear Antibodies  >1 publications with EPAS1 and Ear
 Kidney Antibodies  >1 publications with EPAS1 and Kidney
 Umbilical Cord Antibodies  >1 publications with EPAS1 and Umbilical Cord
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