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ENO1 elisa kit :: Human enolase 1, (alpha) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS706020
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

enolase 1, (alpha) (ENO1), ELISA Kit

★Popular Item★
 Also Known As   

Human Alpha-enolase, ENO1/ENO1L1/MBPB1/MPB1 ELISA Kit

 Product Synonym Names    Human Alpha-enolase (ENO1/ENO1L1/MBPB1/MPB1) ELISA kit; ENO1L1; MBP-1; MPB1; NNE; PPH; 2-phospho-D-glycerate hydro-lyase; MYC promoter-binding protein 1; alpha enolase like 1; enolase 1; non-neural enolase; phosphopyruvate hydratase; tau-crystallin; enolase 1; (alpha)
 Product Gene Name   

ENO1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS706020 Testing Data    Testing Data PDF
 OMIM    172430
 3D Structure    ModBase 3D Structure for P06733
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human ENO1/ENO1L1/MBPB1/MPB1. No significant cross-reactivity or interference between human ENO1/ENO1L1/MBPB1/MPB1 and analogues was observed.
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 Samples    Serum, tissue homogenates, plasma.
 Assay Type    Sandwich (Quantitative)
 Detection Range    0.312 ng/ml-20 ng/ml.
 Sensitivity    0.078 ng/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ENO1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ENO1 purchase    MBS706020 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the enolase 1, (alpha) (ENO1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312 ng/ml-20 ng/ml in biological research samples containing ENO1, with an estimated sensitivity of 0.078 ng/ml. The ELISA analytical biochemical technique of the MBS706020 kit is based on ENO1 antibody-ENO1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ENO1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ENO1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, tissue homogenates, plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ENO1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ENO1/ENO1L1/MBPB1/MPB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ENO1/ENO1L1/MBPB1/MPB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ENO1/ENO1L1/MBPB1/MPB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ENO1/ENO1L1/MBPB1/MPB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ENO1 elisa kit    ENO1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS706020. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ENO1. It may not necessarily be applicable to this product.
 NCBI GI #    319996655
 NCBI GeneID    2023
 NCBI Accession #    NP_001188412.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001201483.1 [Other Products]
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 UniProt Primary Accession #    P06733 [Other Products]
 UniProt Secondary Accession #    P22712; Q16704; Q4TUS4; Q53FT9; Q53HR3; Q658M5; Q6GMP2; Q71V37; Q7Z3V6; Q8WU71; B2RD59 [Other Products]
 UniProt Related Accession #    P06733 [Other Products]
 Molecular Weight    47,169 Da
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 NCBI Official Full Name    c-myc promoter-binding protein-1 isoform MBP-1
 NCBI Official Synonym Full Names    enolase 1, (alpha)
 NCBI Official Symbol    ENO1 [Similar Products]
 NCBI Official Synonym Symbols   
NNE; PPH; MPB1; ENO1L1
[Similar Products]
 NCBI Protein Information    alpha-enolase; c-myc promoter-binding protein-1; enolase-alpha; tau-crystallin; non-neural enolase; alpha enolase like 1; phosphopyruvate hydratase; plasminogen-binding protein; MYC promoter-binding protein 1; 2-phospho-D-glycerate hydro-lyase
 UniProt Protein Name    Alpha-enolase
 UniProt Synonym Protein Names   
2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein
 Protein Family    Enolase
 UniProt Gene Name    ENO1 [Similar Products]
 UniProt Synonym Gene Names    ENO1L1; MBPB1; MPB1; NNE [Similar Products]
 UniProt Entry Name    ENOA_HUMAN
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 NCBI Summary for ENO1    This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]
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 UniProt Comments for ENO1    Function: Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.5 PublicationsManual assertion based on experiment in:Ref.3

Catalytic activity: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O.1 PublicationManual assertion based on experiment in:Ref.18

Cofactor: Magnesium. Binds two Mg2+ per subunit. Required for catalysis and for stabilizing the dimer.1 PublicationManual assertion based on experiment in:Ref.39
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 Research Articles on ENO1    1. Citrullination of ENO1 is associated with neoplasms.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ENO1 elisa kitPathways associated with ENO1 elisa kit
 Reference Product  PubMed Publications
 FLNA elisa kit  >12 publications with ENO1 and FLNA
 Products by Pathway  Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Gluconeogenesis Pathway antibodies  Gluconeogenesis Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
 Glycogen Storage Diseases Pathway antibodies  Glycogen Storage Diseases Pathway Diagram
Diseases associated with ENO1 elisa kitOrgans/Tissues associated with ENO1 elisa kit
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >38 publications with ENO1 and Carcinoma
 Lung Neoplasms Antibodies  >21 publications with ENO1 and Lung Neoplasms
 Brain Diseases Antibodies  >18 publications with ENO1 and Brain Diseases
 Adenocarcinoma Antibodies  >13 publications with ENO1 and Adenocarcinoma
 Arthritis, Rheumatoid Antibodies  >13 publications with ENO1 and Arthritis, Rheumatoid
 Breast Neoplasms Antibodies  >13 publications with ENO1 and Breast Neoplasms
 Carcinoma, Non-Small-Cell Lung Antibodies  >12 publications with ENO1 and Carcinoma, Non-Small-Cell Lung
 Inflammation Antibodies  >12 publications with ENO1 and Inflammation
 Neoplasms, Experimental Antibodies  >9 publications with ENO1 and Neoplasms, Experimental
 Carcinoma, Squamous Cell Antibodies  >7 publications with ENO1 and Carcinoma, Squamous Cell
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with ENO1 and Bone
 Connective Tissue Antibodies  >4 publications with ENO1 and Connective Tissue
 Heart Antibodies  >3 publications with ENO1 and Heart
 Muscle Antibodies  >3 publications with ENO1 and Muscle
 Brain Antibodies  >3 publications with ENO1 and Brain
 Vascular Antibodies  >2 publications with ENO1 and Vascular
 Embryonic Tissue Antibodies  >2 publications with ENO1 and Embryonic Tissue
 Ear Antibodies  >1 publications with ENO1 and Ear
 Kidney Antibodies  >1 publications with ENO1 and Kidney
 Umbilical Cord Antibodies  >1 publications with ENO1 and Umbilical Cord
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