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FADS2 elisa kit :: Human fatty acid desaturase 2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS930393
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

fatty acid desaturase 2 (FADS2), ELISA Kit

★Popular Item★
 Also Known As   

Human Fatty acid desaturase 2, FADS2 ELISA Kit

 Product Synonym Names    Human Fatty acid desaturase 2 (FADS2) ELISA kit; D6D; DES6; FADSD6; LLCDL2; SLL0262; TU13; delta-6 fatty acid desaturase; delta-6-desaturase; linoleoyl-CoA desaturase (delta-6-desaturase) -like 2; fatty acid desaturase 2
 Product Gene Name   

FADS2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS930393 Testing Data    Testing Data PDF
 OMIM    606149
 3D Structure    ModBase 3D Structure for O95864
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human FADS2. No significant cross-reactivity or interference between human FADS2 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    31.2 pg/ml-2000 pg/ml.
 Sensitivity    The minimum detectable dose of human FADS2 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FADS2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FADS2 purchase    MBS930393 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the fatty acid desaturase 2 (FADS2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2 pg/ml-2000 pg/ml in biological research samples containing FADS2, with an estimated sensitivity of The minimum detectable dose of human FADS2 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS930393 kit is based on FADS2 antibody-FADS2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FADS2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FADS2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for FADS2 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FADS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FADS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FADS2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FADS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of FADS2 elisa kit    FADS2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS930393. Click to request current manual
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NCBI/Uniprot data below describe general gene information for FADS2. It may not necessarily be applicable to this product.
 NCBI GI #    527498295
 NCBI GeneID    9415
 NCBI Accession #    NP_001268430.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001281501.1 [Other Products]
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 UniProt Primary Accession #    O95864 [Other Products]
 UniProt Secondary Accession #    Q6MZQ7; Q96H07; Q96SV8; Q9H3G3; Q9Y3X4; A8K2M6; B7Z634 [Other Products]
 UniProt Related Accession #    O95864 [Other Products]
 Molecular Weight    52,259 Da
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 NCBI Official Full Name    fatty acid desaturase 2 isoform 2
 NCBI Official Synonym Full Names    fatty acid desaturase 2
 NCBI Official Symbol    FADS2 [Similar Products]
 NCBI Official Synonym Symbols   
D6D; DES6; TU13; FADSD6; LLCDL2; SLL0262
[Similar Products]
 NCBI Protein Information    fatty acid desaturase 2; delta-6 desaturase; delta-6-desaturase; delta(6) desaturase; delta-6 fatty acid desaturase; delta(6) fatty acid desaturase; linoleoyl-CoA desaturase (delta-6-desaturase)-like 2
 UniProt Protein Name    Fatty acid desaturase 2
 UniProt Synonym Protein Names   
Delta(6) fatty acid desaturase; D6D; Delta(6) desaturase; Delta-6 desaturase
 Protein Family    Fatty acid desaturase
 UniProt Gene Name    FADS2 [Similar Products]
 UniProt Synonym Gene Names    D6D; Delta(6) desaturase; Delta-6 desaturase [Similar Products]
 UniProt Entry Name    FADS2_HUMAN
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 NCBI Summary for FADS2    The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]
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 UniProt Comments for FADS2    FADS2: Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma- linoleic acid (GLA) (18:3n-6) and stearidonic acid (18:4n-3) respectively and other desaturation steps. Highly unsaturated fatty acids (HUFA) play pivotal roles in many biological functions. It catalizes as well the introduction of a cis double bond in palmitate to produce the mono-unsaturated fatty acid sapienate, the most abundant fatty acid in sebum. Belongs to the fatty acid desaturase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.19.-; Oxidoreductase; Membrane protein, integral; Lipid Metabolism - unsaturated fatty acid biosynthesis; Lipid Metabolism - alpha-linolenic acid; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11q12.2

Cellular Component: endoplasmic reticulum membrane; membrane; integral to plasma membrane

Molecular Function: iron ion binding; heme binding; stearoyl-CoA 9-desaturase activity

Biological Process: unsaturated fatty acid metabolic process; linoleic acid metabolic process; unsaturated fatty acid biosynthetic process
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 Research Articles on FADS2    1. The results of this study indicate that a human-specific haplotype of the FADS2 gene cluster, which increases the synthesis of omega-3 and omega-6 LC-PUFAs (Ameur et al., 2012), is associated with age-related WM differences from childhood to early adulthood.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with FADS2 elisa kitPathways associated with FADS2 elisa kit
 Reference Product  PubMed Publications
 ELOVL5 elisa kit  >19 publications with FADS2 and ELOVL5
 ELOVL2 elisa kit  >18 publications with FADS2 and ELOVL2
 ACSL1 elisa kit  >2 publications with FADS2 and ACSL1
 Products by Pathway  Pathway Diagram
 Biosynthesis Of Unsaturated Fatty Acids Pathway antibodies  Biosynthesis Of Unsaturated Fatty Acids Pathway Diagram
 Biosynthesis Of Unsaturated Fatty Acids Pathway antibodies  Biosynthesis Of Unsaturated Fatty Acids Pathway Diagram
 Fatty Acid Metabolism Pathway antibodies  Fatty Acid Metabolism Pathway Diagram
 Fatty Acid Metabolism Pathway antibodies  Fatty Acid Metabolism Pathway Diagram
 Linoleic Acid (LA) Metabolism Pathway antibodies  Linoleic Acid (LA) Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram
 Alpha-Linolenic Acid Metabolism Pathway antibodies  Alpha-Linolenic Acid Metabolism Pathway Diagram
Diseases associated with FADS2 elisa kitOrgans/Tissues associated with FADS2 elisa kit
 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >44 publications with FADS2 and Fatty Liver
 Cardiovascular Diseases Antibodies  >22 publications with FADS2 and Cardiovascular Diseases
 Inflammation Antibodies  >17 publications with FADS2 and Inflammation
 Heart Diseases Antibodies  >14 publications with FADS2 and Heart Diseases
 Liver Diseases Antibodies  >9 publications with FADS2 and Liver Diseases
 Atherosclerosis Antibodies  >5 publications with FADS2 and Atherosclerosis
 Carcinoma, Hepatocellular Antibodies  >4 publications with FADS2 and Carcinoma, Hepatocellular
 Nervous System Diseases Antibodies  >3 publications with FADS2 and Nervous System Diseases
 Fibrosis Antibodies  >3 publications with FADS2 and Fibrosis
 Depressive Disorder Antibodies  >3 publications with FADS2 and Depressive Disorder
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >47 publications with FADS2 and Blood
 Liver Antibodies  >24 publications with FADS2 and Liver
 Brain Antibodies  >11 publications with FADS2 and Brain
 Heart Antibodies  >9 publications with FADS2 and Heart
 Adipose Tissue Antibodies  >6 publications with FADS2 and Adipose Tissue
 Skin Antibodies  >6 publications with FADS2 and Skin
 Muscle Antibodies  >4 publications with FADS2 and Muscle
 Vascular Antibodies  >4 publications with FADS2 and Vascular
 Connective Tissue Antibodies  >3 publications with FADS2 and Connective Tissue
 Intestine Antibodies  >3 publications with FADS2 and Intestine
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