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FOXO3 elisa kit :: Human forkhead box O3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS701142
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

forkhead box O3 (FOXO3), ELISA Kit

 Also Known As   

Human forkhead box O3, FOXO3 ELISA Kit

 Product Synonym Names    Human forkhead box O3; FOXO3 ELISA Kit; AF6q21; DKFZp781A0677; FKHRL1; FKHRL1P2; FOXO2; FOXO3A; MGC12739; MGC31925; forkhead box O3A; forkhead homolog (rhabdomyosarcoma) like 1; forkhead; Drosophila; homolog of; in rhabdomyosarcoma-like 1; forkhead box O3
 Product Gene Name   

FOXO3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS701142 Testing Data    Testing Data PDF
 OMIM    602681
 3D Structure    ModBase 3D Structure for O43524
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human FOXO3. No significant cross-reactivity or interference between Human FOXO3 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Detection Range    1.56 pg/ml -100 pg/ml
 Sensitivity    0.39 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Apoptosis/Autophagy
 Protein Biological Process 3    Apoptosis
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FOXO3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FOXO3 purchase    MBS701142 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the forkhead box O3 (FOXO3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.56 pg/ml -100 pg/ml in biological research samples containing FOXO3, with an estimated sensitivity of 0.39 pg/ml. The ELISA analytical biochemical technique of the MBS701142 kit is based on FOXO3 antibody-FOXO3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FOXO3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FOXO3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for FOXO3 elisa kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FOXO3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for FOXO3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain FOXO3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of FOXO3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of FOXO3 elisa kit    FOXO3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS701142. Click to request current manual
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NCBI/Uniprot data below describe general gene information for FOXO3. It may not necessarily be applicable to this product.
 NCBI GI #    4503739
 NCBI GeneID    2309
 NCBI Accession #    NP_001446.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001455.3 [Other Products]
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 UniProt Primary Accession #    O43524 [Other Products]
 UniProt Secondary Accession #    O15171; Q5T2I7; Q9BZ04; E1P5E6 [Other Products]
 UniProt Related Accession #    O43524 [Other Products]
 Molecular Weight    71,277 Da
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 NCBI Official Full Name    forkhead box protein O3
 NCBI Official Synonym Full Names    forkhead box O3
 NCBI Official Symbol    FOXO3 [Similar Products]
 NCBI Official Synonym Symbols   
FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
[Similar Products]
 NCBI Protein Information    forkhead box protein O3; forkhead box O3A; forkhead in rhabdomyosarcoma-like 1; forkhead homolog (rhabdomyosarcoma) like 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
 UniProt Protein Name    Forkhead box protein O3
 UniProt Synonym Protein Names   
AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1
 Protein Family    Forkhead box protein
 UniProt Gene Name    FOXO3 [Similar Products]
 UniProt Synonym Gene Names    FKHRL1; FOXO3A [Similar Products]
 UniProt Entry Name    FOXO3_HUMAN
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 NCBI Summary for FOXO3    This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
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 UniProt Comments for FOXO3    Function: Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.3 PublicationsManual assertion based on experiment in:Ref.6
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 Research Articles on FOXO3    1. These data provide evidence for an essential role of FoxO proteins in the transcription of ABCA6 in human vascular endothelial cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with FOXO3 elisa kitPathways associated with FOXO3 elisa kit
 Reference Product  PubMed Publications
 SIRT1 elisa kit  >66 publications with FOXO3 and SIRT1
 AKT1 elisa kit  >58 publications with FOXO3 and AKT1
 CTNNB1 elisa kit  >22 publications with FOXO3 and CTNNB1
 IKBKB elisa kit  >18 publications with FOXO3 and IKBKB
 SMAD4 elisa kit  >6 publications with FOXO3 and SMAD4
 SMAD2 elisa kit  >5 publications with FOXO3 and SMAD2
 BCL2L11 elisa kit  >4 publications with FOXO3 and BCL2L11
 YWHAZ elisa kit  >1 publications with FOXO3 and YWHAZ
 Products by Pathway  Pathway Diagram
 AKT Phosphorylates Targets In The Nucleus Pathway antibodies  AKT Phosphorylates Targets In The Nucleus Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class I PI3K Signaling Events Pathway antibodies  Class I PI3K Signaling Events Pathway Diagram
 Class I PI3K Signaling Events Mediated By Akt Pathway antibodies  Class I PI3K Signaling Events Mediated By Akt Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
Diseases associated with FOXO3 elisa kitOrgans/Tissues associated with FOXO3 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >85 publications with FOXO3 and Nervous System Diseases
 Atrophy Antibodies  >85 publications with FOXO3 and Atrophy
 Breast Neoplasms Antibodies  >57 publications with FOXO3 and Breast Neoplasms
 Inflammation Antibodies  >55 publications with FOXO3 and Inflammation
 Cardiovascular Diseases Antibodies  >55 publications with FOXO3 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >50 publications with FOXO3 and Disease Models, Animal
 Necrosis Antibodies  >48 publications with FOXO3 and Necrosis
 Adenocarcinoma Antibodies  >42 publications with FOXO3 and Adenocarcinoma
 Heart Diseases Antibodies  >32 publications with FOXO3 and Heart Diseases
 Hypertrophy Antibodies  >29 publications with FOXO3 and Hypertrophy
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >114 publications with FOXO3 and Muscle
 Blood Antibodies  >109 publications with FOXO3 and Blood
 Vascular Antibodies  >46 publications with FOXO3 and Vascular
 Brain Antibodies  >45 publications with FOXO3 and Brain
 Heart Antibodies  >39 publications with FOXO3 and Heart
 Ovary Antibodies  >28 publications with FOXO3 and Ovary
 Lung Antibodies  >26 publications with FOXO3 and Lung
 Bone Antibodies  >26 publications with FOXO3 and Bone
 Liver Antibodies  >26 publications with FOXO3 and Liver
 Nerve Antibodies  >24 publications with FOXO3 and Nerve
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