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GLA elisa kit :: Human galactosidase, alpha ELISA Kit

Scan QR to view Datasheet Catalog #    MBS702399
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

galactosidase, alpha (GLA), ELISA Kit

★Popular Item★
 Also Known As   

Human alpha-galactosidase, alphaGAL ELISA Kit

 Product Synonym Names    Human alpha-galactosidase; alphaGAL ELISA Kit; GALA; agalsidase alfa; alpha-D-galactosidase A; alpha-D-galactoside galactohydrolase 1; alpha-gal A; alpha-galactosidase A; melibiase; galactosidase; alpha
 Product Gene Name   

GLA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS702399 Testing Data    Testing Data PDF
 OMIM    gene 301500
 3D Structure    ModBase 3D Structure for P06280
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human alphaGAL. No significant cross-reactivity or interference between human alphaGAL and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates.
 Assay Type    Sandwich (Quantitative)
 Detection Range    7.8 pg/ml-500 pg/ml.
 Sensitivity    1.95 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GLA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GLA purchase    MBS702399 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the galactosidase, alpha (GLA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 7.8 pg/ml-500 pg/ml in biological research samples containing GLA, with an estimated sensitivity of 1.95 pg/ml. The ELISA analytical biochemical technique of the MBS702399 kit is based on GLA antibody-GLA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GLA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GLA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GLA elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for alphaGAL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any alphaGAL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for alphaGAL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of alphaGAL bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of GLA elisa kit    GLA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS702399. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GLA. It may not necessarily be applicable to this product.
 NCBI GI #    4504009
 NCBI GeneID    2717
 NCBI Accession #    NP_000160.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000169.2 [Other Products]
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 UniProt Primary Accession #    P06280 [Other Products]
 UniProt Secondary Accession #    Q6LER7 [Other Products]
 UniProt Related Accession #    P06280 [Other Products]
 Molecular Weight    48,767 Da
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 NCBI Official Full Name    alpha-galactosidase A
 NCBI Official Synonym Full Names    galactosidase, alpha
 NCBI Official Symbol    GLA [Similar Products]
 NCBI Official Synonym Symbols   
GALA
[Similar Products]
 NCBI Protein Information    alpha-galactosidase A; melibiase; alpha-gal A; agalsidase alfa; alpha-D-galactosidase A; alpha-D-galactoside galactohydrolase 1
 UniProt Protein Name    Alpha-galactosidase A
 UniProt Synonym Protein Names   
Alpha-D-galactosidase A; Alpha-D-galactoside galactohydrolase; Melibiase; INN: Agalsidase
 Protein Family    Glycerol facilitator-aquaporin
 UniProt Gene Name    GLA [Similar Products]
 UniProt Entry Name    AGAL_HUMAN
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 NCBI Summary for GLA    This gene encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties. [provided by RefSeq, Jul 2008]
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 UniProt Comments for GLA    GLA: Defects in GLA are the cause of Fabry disease (FD). FD is a rare X-linked sphingolipidosis disease where glycolipid accumulates in many tissues. The disease consists of an inborn error of glycosphingolipid catabolism. FD patients show systemic accumulation of globotriaoslyceramide (Gb3) and related glycosphingolipids in the plasma and cellular lysosomes throughout the body. Clinical recognition in males results from characteristic skin lesions (angiokeratomas) over the lower trunk. Patients may show ocular deposits, febrile episodes, and burning pain in the extremities. Death results from renal failure, cardiac or cerebral complications of hypertension or other vascular disease. Heterozygous females may exhibit the disorder in an attenuated form, they are more likely to show corneal opacities. Belongs to the glycosyl hydrolase 27 family.

Protein type: Glycan Metabolism - glycosphingolipid biosynthesis - globo series; Hydrolase; Carbohydrate Metabolism - galactose; Lipid Metabolism - glycerolipid; EC 3.2.1.22; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: Xq22

Cellular Component: Golgi apparatus; lysosomal lumen; lysosome; cytoplasm; extracellular region

Molecular Function: protein binding; protein homodimerization activity; hydrolase activity; alpha-galactosidase activity; galactoside binding; catalytic activity; receptor binding

Biological Process: sphingolipid metabolic process; negative regulation of nitric-oxide synthase activity; glycoside catabolic process; negative regulation of nitric oxide biosynthetic process; glycosphingolipid catabolic process; glycosylceramide catabolic process; glycosphingolipid metabolic process; oligosaccharide metabolic process

Disease: Fabry Disease
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 Research Articles on GLA    1. Missense mutation p.R112C ablates the activity of the alpha-gal A gene and results in the development of Fabry disease with renal damage in a Chinese family.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GLA elisa kitPathways associated with GLA elisa kit
 Reference Product  PubMed Publications
 GAA elisa kit  >8 publications with GLA and GAA
 ARSA elisa kit  >4 publications with GLA and ARSA
 HEXA elisa kit  >3 publications with GLA and HEXA
 GALC elisa kit  >3 publications with GLA and GALC
 GLB1 elisa kit  >2 publications with GLA and GLB1
 HEXB elisa kit  >1 publications with GLA and HEXB
 Products by Pathway  Pathway Diagram
 Galactose Metabolism Pathway antibodies  Galactose Metabolism Pathway Diagram
 Galactose Metabolism Pathway antibodies  Galactose Metabolism Pathway Diagram
 Glycerolipid Metabolism Pathway antibodies  Glycerolipid Metabolism Pathway Diagram
 Glycerolipid Metabolism Pathway antibodies  Glycerolipid Metabolism Pathway Diagram
 Glycosphingolipid Biosynthesis - Globo Series Pathway antibodies  Glycosphingolipid Biosynthesis - Globo Series Pathway Diagram
 Glycosphingolipid Biosynthesis - Globo Series Pathway antibodies  Glycosphingolipid Biosynthesis - Globo Series Pathway Diagram
 Glycosphingolipid Metabolism Pathway antibodies  Glycosphingolipid Metabolism Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
Diseases associated with GLA elisa kitOrgans/Tissues associated with GLA elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >1195 publications with GLA and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >959 publications with GLA and Nervous System Diseases
 Heart Diseases Antibodies  >415 publications with GLA and Heart Diseases
 Inflammation Antibodies  >331 publications with GLA and Inflammation
 Disease Models, Animal Antibodies  >261 publications with GLA and Disease Models, Animal
 Kidney Diseases Antibodies  >249 publications with GLA and Kidney Diseases
 Hypertension Antibodies  >236 publications with GLA and Hypertension
 Fabry Disease Antibodies  >206 publications with GLA and Fabry Disease
 Necrosis Antibodies  >171 publications with GLA and Necrosis
 Pain Antibodies  >162 publications with GLA and Pain
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with GLA and Bone
 Connective Tissue Antibodies  >4 publications with GLA and Connective Tissue
 Heart Antibodies  >3 publications with GLA and Heart
 Muscle Antibodies  >3 publications with GLA and Muscle
 Brain Antibodies  >3 publications with GLA and Brain
 Vascular Antibodies  >2 publications with GLA and Vascular
 Embryonic Tissue Antibodies  >2 publications with GLA and Embryonic Tissue
 Ear Antibodies  >1 publications with GLA and Ear
 Kidney Antibodies  >1 publications with GLA and Kidney
 Umbilical Cord Antibodies  >1 publications with GLA and Umbilical Cord
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