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GGT1 elisa kit :: Human gamma-glutamyltransferase 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS704964
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

gamma-glutamyltransferase 1 (GGT1), ELISA Kit

 Also Known As   

Human gamma glutamyl transpeptidase, GGT ELISA Kit

 Product Synonym Names    Human gamma glutamyl transpeptidase; GGT ELISA Kit; CD224; D22S672; D22S732; GGT; GTG; MGC96892; MGC96904; MGC96963; OTTHUMP00000028921; OTTHUMP00000159078; gamma-glutamyl transpeptidase; glutamyl transpeptidase; gamma-glutamyltransferase 1
 Product Gene Name   

GGT1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS704964 Testing Data    Testing Data PDF
 OMIM    612346
 3D Structure    ModBase 3D Structure for P19440
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human GGT1. No significant cross-reactivity or interference between Human GGT1 and analogues was observed.
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 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Biosynthesis/Metabolism
 Protein Biological Process 2    Amino-acid biosynthesis and metabolism
 Protein Biological Process 3    Glutathione biosynthesis
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GGT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GGT1 purchase    MBS704964 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the gamma-glutamyltransferase 1 (GGT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GGT1. The ELISA analytical biochemical technique of the MBS704964 kit is based on GGT1 antibody-GGT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GGT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GGT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for GGT1 elisa kit

   Introduction: Gamma-glutamyl transferase (GGT or GGTP, or Gamma-GT) is involved in the transfer of amino acids across the cellular membrane. It is also involved in glutathione metabolism by transferring the glutamyl moiety to a variety of acceptor molecules including water, certain L-amino acids and peptides. Leaving the cysteine product to preserve intracellular homeostasis of oxidative stress. GGT is found on the cell surface on all cells, with particularly high concentrations in the liver, bile ducts, and kidney. The enzyme is also present in other tissues, such as the epididymis. Elevated GGT is a marker of recent alcohol misuse. It may indicate excess alcohol consumption up to 3 or 4 weeks prior to the test. It is also used as a diagnostic marker for other types of liver damage or conditions attributable to other causes unrelated to alcohol abuse.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GGT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for GGT and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain GGT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GGT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of GGT1 elisa kit    GGT1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS704964. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GGT1. It may not necessarily be applicable to this product.
 NCBI GI #    572152963
 NCBI GeneID    2678
 NCBI Accession #    NP_001275762.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001288833.1 [Other Products]
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 UniProt Primary Accession #    P19440 [Other Products]
 UniProt Secondary Accession #    Q08247; Q14404; Q8TBS1; Q9UMK1 [Other Products]
 UniProt Related Accession #    P19440 [Other Products]
 Molecular Weight    61,410 Da
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 NCBI Official Full Name    gamma-glutamyltranspeptidase 1
 NCBI Official Synonym Full Names    gamma-glutamyltransferase 1
 NCBI Official Symbol    GGT1 [Similar Products]
 NCBI Official Synonym Symbols   
GGT; GTG; CD224; GGT 1; D22S672; D22S732
[Similar Products]
 NCBI Protein Information    gamma-glutamyltranspeptidase 1; glutathione hydrolase 1; leukotriene-C4 hydrolase
 UniProt Protein Name    Gamma-glutamyltranspeptidase 1
 UniProt Synonym Protein Names   
Gamma-glutamyltransferase 1; Glutathione hydrolase 1 (EC:3.4.19.13); Leukotriene-C4 hydrolase (EC:3.4.19.14); CD_antigen: CD224Cleaved into the following 2 chains:Gamma-glutamyltranspeptidase 1 heavy chain; Gamma-glutamyltranspeptidase 1 light chain
 Protein Family    Gamma-glutamyltranspeptidase
 UniProt Gene Name    GGT1 [Similar Products]
 UniProt Synonym Gene Names    GGT; GGT 1 [Similar Products]
 UniProt Entry Name    GGT1_HUMAN
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 NCBI Summary for GGT1    The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22. [provided by RefSeq, Jan 2014]
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 UniProt Comments for GGT1    Function: Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates, and other gamma-glutamyl compounds. The metabolism of glutathione releases free glutamate and the dipeptide, cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Isoform 3 seems to be inactive.7 PublicationsManual assertion based on experiment in:Ref.7

Catalytic activity: A (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid.8 PublicationsManual assertion based on experiment in:Ref.15

Enzyme regulation: Activated by autocatalytic cleavage.1 PublicationManual assertion based on experiment in:Ref.24
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 Research Articles on GGT1    1. The epidemiological association of alcohol intake with GGT is partly explained by genetic pleiotropy in this healthy population sample.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GGT1 elisa kitPathways associated with GGT1 elisa kit
 Reference Product  PubMed Publications
 GPX1 elisa kit  >1 publications with GGT1 and GPX1
 GSTA1 elisa kit  >1 publications with GGT1 and GSTA1
 Products by Pathway  Pathway Diagram
 Aflatoxin Activation And Detoxification Pathway antibodies  Aflatoxin Activation And Detoxification Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 Cyanoamino Acid Metabolism Pathway antibodies  Cyanoamino Acid Metabolism Pathway Diagram
 Cyanoamino Acid Metabolism Pathway antibodies  Cyanoamino Acid Metabolism Pathway Diagram
 Eicosanoid Synthesis Pathway antibodies  Eicosanoid Synthesis Pathway Diagram
 Glutathione Conjugation Pathway antibodies  Glutathione Conjugation Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
Diseases associated with GGT1 elisa kitOrgans/Tissues associated with GGT1 elisa kit
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >8 publications with GGT1 and Liver Diseases
 Carcinoma Antibodies  >6 publications with GGT1 and Carcinoma
 Breast Neoplasms Antibodies  >4 publications with GGT1 and Breast Neoplasms
 Liver Neoplasms Antibodies  >3 publications with GGT1 and Liver Neoplasms
 Heart Diseases Antibodies  >2 publications with GGT1 and Heart Diseases
 Lung Neoplasms Antibodies  >1 publications with GGT1 and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >1 publications with GGT1 and Neoplasms, Experimental
 Carcinoma, Hepatocellular Antibodies  >1 publications with GGT1 and Carcinoma, Hepatocellular
 Inflammation Antibodies  >1 publications with GGT1 and Inflammation
 Liver Neoplasms, Experimental Antibodies  >1 publications with GGT1 and Liver Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with GGT1 and Bone
 Connective Tissue Antibodies  >4 publications with GGT1 and Connective Tissue
 Heart Antibodies  >3 publications with GGT1 and Heart
 Muscle Antibodies  >3 publications with GGT1 and Muscle
 Brain Antibodies  >3 publications with GGT1 and Brain
 Vascular Antibodies  >2 publications with GGT1 and Vascular
 Embryonic Tissue Antibodies  >2 publications with GGT1 and Embryonic Tissue
 Ear Antibodies  >1 publications with GGT1 and Ear
 Kidney Antibodies  >1 publications with GGT1 and Kidney
 Umbilical Cord Antibodies  >1 publications with GGT1 and Umbilical Cord
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