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GCK elisa kit :: Human glucokinase (hexokinase 4) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS701446
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

glucokinase (hexokinase 4) (GCK), ELISA Kit

 Also Known As   

Human glucokinase, GCK ELISA Kit

 Product Synonym Names    Human glucokinase; GCK ELISA Kit; GK; GLK; HHF3; HK4; HKIV; HXKP; MODY2; ATP:D-hexose 6-phosphotransferase; glucokinase; hexokinase D; pancreatic isozyme; glucokinase (hexokinase 4)
 Product Gene Name   

GCK elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS701446 Testing Data    Testing Data PDF
 OMIM    606391
 3D Structure    ModBase 3D Structure for P35557
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human GCK. No significant cross-reactivity or interference between human GCK and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    64 pg/ml -1000 pg/ml.
 Sensitivity    The minimum detectable dose of human GCK is typically less than 22 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human GCK concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GCK elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GCK purchase    MBS701446 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the glucokinase (hexokinase 4) (GCK) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 64 pg/ml -1000 pg/ml in biological research samples containing GCK, with an estimated sensitivity of The minimum detectable dose of human GCK is typically less than 22 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human GCK concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS701446 kit is based on GCK antibody-GCK antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GCK antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GCK. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GCK elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GCK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for GCK. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GCK bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of GCK elisa kit    GCK elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS701446. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GCK. It may not necessarily be applicable to this product.
 NCBI GI #    4503951
 NCBI GeneID    2645
 NCBI Accession #    NP_000153.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000162.3 [Other Products]
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 UniProt Primary Accession #    P35557 [Other Products]
 UniProt Secondary Accession #    Q05810; A4D2J2; A4D2J3 [Other Products]
 UniProt Related Accession #    P35557 [Other Products]
 Molecular Weight    52,191 Da
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 NCBI Official Full Name    glucokinase isoform 1
 NCBI Official Synonym Full Names    glucokinase (hexokinase 4)
 NCBI Official Symbol    GCK [Similar Products]
 NCBI Official Synonym Symbols   
GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3
[Similar Products]
 NCBI Protein Information    glucokinase; HK IV; hexokinase-4; hexokinase-D; hexokinase type IV; hexokinase D, pancreatic isozyme; ATP:D-hexose 6-phosphotransferase
 UniProt Protein Name    Glucokinase
 UniProt Synonym Protein Names   
Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D
 Protein Family    Glycerate 2-kinase
 UniProt Gene Name    GCK [Similar Products]
 UniProt Synonym Gene Names    HK IV; HK4 [Similar Products]
 UniProt Entry Name    HXK4_HUMAN
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 NCBI Summary for GCK    Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq, Apr 2009]
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 UniProt Comments for GCK    Function: Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage.

Catalytic activity: ATP + D-glucose = ADP + D-glucose 6-phosphate.

Enzyme regulation: The use of alternative promoters apparently enables the type IV hexokinase gene to be regulated by insulin in the liver and glucose in the beta cell. This may constitute an important feedback loop for maintaining glucose homeostasis. Subject to allosteric regulation. Low glucose and high fructose-6-phosphate triggers association with the inhibitor GKRP followed by sequestration in the nucleus.2 PublicationsManual assertion based on experiment in:Ref.11
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 Research Articles on GCK    1. On mutation of glucokinase, a hydrogen bond between the R275 side-chain and the carbonyl oxygen of D267 is broken, destabilizing the F260-L271 loop structure and the protein.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GCK elisa kitPathways associated with GCK elisa kit
 Reference Product  PubMed Publications
 GCKR elisa kit  >40 publications with GCK and GCKR
 INS elisa kit  >31 publications with GCK and INS
 SREBF1 elisa kit  >7 publications with GCK and SREBF1
 GPI elisa kit  >2 publications with GCK and GPI
 PKLR elisa kit  >1 publications with GCK and PKLR
 Products by Pathway  Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Butirosin And Neomycin Biosynthesis Pathway antibodies  Butirosin And Neomycin Biosynthesis Pathway Diagram
 Butirosin And Neomycin Biosynthesis Pathway antibodies  Butirosin And Neomycin Biosynthesis Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies  FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
 GDP-glucose Biosynthesis Pathway antibodies  GDP-glucose Biosynthesis Pathway Diagram
Diseases associated with GCK elisa kitOrgans/Tissues associated with GCK elisa kit
 Disease Name  Pubmed Publications
 Diabetes Mellitus, Type 2 Antibodies  >205 publications with GCK and Diabetes Mellitus, Type 2
 Hyperglycemia Antibodies  >129 publications with GCK and Hyperglycemia
 Congenital Hyperinsulinism Antibodies  >34 publications with GCK and Congenital Hyperinsulinism
 Cardiovascular Diseases Antibodies  >17 publications with GCK and Cardiovascular Diseases
 Necrosis Antibodies  >16 publications with GCK and Necrosis
 Maturity-Onset Diabetes of the Young, Type 2 Antibodies  >15 publications with GCK and Maturity-Onset Diabetes of the Young, Type 2
 Fatty Liver Antibodies  >15 publications with GCK and Fatty Liver
 Diabetes Mellitus, Experimental Antibodies  >15 publications with GCK and Diabetes Mellitus, Experimental
 Kidney Diseases Antibodies  >11 publications with GCK and Kidney Diseases
 Liver Diseases Antibodies  >11 publications with GCK and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >76 publications with GCK and Pancreas
 Liver Antibodies  >47 publications with GCK and Liver
 Brain Antibodies  >22 publications with GCK and Brain
 Kidney Antibodies  >11 publications with GCK and Kidney
 Embryonic Tissue Antibodies  >5 publications with GCK and Embryonic Tissue
 Lymph Node Antibodies  >4 publications with GCK and Lymph Node
 Eye Antibodies  >3 publications with GCK and Eye
 Adipose Tissue Antibodies  >2 publications with GCK and Adipose Tissue
 Placenta Antibodies  >1 publications with GCK and Placenta
 Lung Antibodies  >1 publications with GCK and Lung
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