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GNE elisa kit :: Human glucosamine (UDP-N-acetyl) -2-epimerase/N-acetylmannosamine kinase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS9323925 GNE elisa kit
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $680 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

glucosamine (UDP-N-acetyl) -2-epimerase/N-acetylmannosamine kinase (GNE), ELISA Kit

★Popular Item★
 Also Known As   

Human Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, GNE ELISA Kit

 Product Synonym Names    Human Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE) ELISA kit; DMRV; GLCNE; IBM2; NM; Uae1; N-acylmannosamine kinase; UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase; glucosamine (UDP-N-acetyl) -2-epimerase/N-acetylmannosamine kinase
 Product Gene Name   

GNE elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 605820
 3D Structure    ModBase 3D Structure for Q9Y223
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 Species Reactivity    Human
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 Specificity    No significant cross-reactivity or interference between this analyte and analogues is observed.
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 Samples    Body fluids, tissue homogenates, secretions or feces samples
 Assay Type    Quantitative Sandwich
 Detection Range    0.625 ng/ml - 20 ng/ml
 Sensitivity    0.1 ng/ml.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100].
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GNE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GNE purchase    MBS9323925 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the glucosamine (UDP-N-acetyl) -2-epimerase/N-acetylmannosamine kinase (GNE) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625 ng/ml - 20 ng/ml in biological research samples containing GNE, with an estimated sensitivity of 0.1 ng/ml. The ELISA analytical biochemical technique of the MBS9323925 kit is based on GNE antibody-GNE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GNE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GNE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Body fluids, tissue homogenates, secretions or feces samples. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GNE elisa kit

   Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of GNE (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Sample Manual Insert of MBS9323925. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GNE. It may not necessarily be applicable to this product.
 NCBI GI #    190014632
 NCBI GeneID    10020
 NCBI Accession #    NP_001121699.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001128227.2 [Other Products]
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 UniProt Primary Accession #    Q9Y223 [Other Products]
 UniProt Secondary Accession #    Q0VA94; A6PZH2; A6PZH3; A7UNU7; B2R6E1; B7Z372; B7Z428; D3DRP7; F5H499; H0YFA7 [Other Products]
 UniProt Related Accession #    Q9Y223 [Other Products]
 Molecular Weight    79,275 Da
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 NCBI Official Full Name    bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 1
 NCBI Official Synonym Full Names    glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
 NCBI Official Symbol    GNE [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; N-acylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase
 UniProt Protein Name    Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
 UniProt Synonym Protein Names   
UDP-GlcNAc-2-epimerase/ManAc kinaseIncluding the following 2 domains:UDP-N-acetylglucosamine 2-epimerase (hydrolyzing) (EC: name(s):UDP-GlcNAc-2-epimerase; Uridine diphosphate-N-acetylglucosamine-2-epimerase
 Protein Family    Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
 UniProt Gene Name    GNE [Similar Products]
 UniProt Synonym Gene Names    GLCNE [Similar Products]
 UniProt Entry Name    GLCNE_HUMAN
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 NCBI Summary for GNE    The protein encoded by this gene is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells. Mutations in this gene are associated with sialuria, autosomal recessive inclusion body myopathy, and Nonaka myopathy. Alternative splicing of this gene results in transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
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 UniProt Comments for GNE    GNE: Regulates and initiates biosynthesis of N- acetylneuraminic acid (NeuAc), a precursor of sialic acids. Plays an essential role in early development. Required for normal sialylation in hematopoietic cells. Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells. Defects in GNE are a cause of sialuria (SIALURIA); also known as sialuria French type. In sialuria, free sialic acid accumulates in the cytoplasm and gram quantities of neuraminic acid are secreted in the urine. The metabolic defect involves lack of feedback inhibition of UDP-GlcNAc 2-epimerase by CMP-Neu5Ac, resulting in constitutive overproduction of free Neu5Ac. Clinical features include variable degrees of developmental delay, coarse facial features and hepatomegaly. Sialuria inheritance is autosomal dominant. Defects in GNE are the cause of inclusion body myopathy type 2 (IBM2). Hereditary inclusion body myopathies are a group of neuromuscular disorders characterized by adult onset, slowly progressive distal and proximal weakness and a typical muscle pathology including rimmed vacuoles and filamentous inclusions. IBM2 is an autosomal recessive disorder affecting mainly leg muscles, but with an unusual distribution that spares the quadriceps as also observed in Nonaka myopathy. Defects in GNE are the cause of Nonaka myopathy (NM); also known as distal myopathy with rimmed vacuoles (DMRV). NM is an autosomal recessive muscular disorder, allelic to inclusion body myopathy 2. It is characterized by weakness of the anterior compartment of the lower limbs with onset in early adulthood, and sparing of the quadriceps muscles. As the inclusion body myopathy, NM is histologically characterized by the presence of numerous rimmed vacuoles without inflammatory changes in muscle specimens. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Motility/polarity/chemotaxis; Isomerase; Cytoskeletal; Kinase, other; Cell adhesion; EC

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: cytoplasm; cytosol

Molecular Function: metal ion binding; UDP-N-acetylglucosamine 2-epimerase activity; ATP binding; hydrolase activity, hydrolyzing O-glycosyl compounds; N-acylmannosamine kinase activity

Biological Process: N-acetylglucosamine biosynthetic process; UDP-N-acetylglucosamine metabolic process; cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; carbohydrate phosphorylation; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; cell adhesion; N-acetylneuraminate metabolic process

Disease: Inclusion Body Myopathy 2, Autosomal Recessive; Nonaka Myopathy; Sialuria
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 Research Articles on GNE    1. we report the mutation profile of the GNE gene in 212 Japanese GNE myopathy patients, which is the largest single-ethnic cohort for this ultra-orphan disease
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GNE elisa kitPathways associated with GNE elisa kit
 Reference Product  PubMed Publications
 ZBTB16 elisa kit  >1 publications with GNE and ZBTB16
 Products by Pathway  Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 CMP-N-acetylneuraminate Biosynthesis I (eukaryotes) Pathway antibodies  CMP-N-acetylneuraminate Biosynthesis I (eukaryotes) Pathway Diagram
Diseases associated with GNE elisa kitOrgans/Tissues associated with GNE elisa kit
 Disease Name  Pubmed Publications
 Muscular Diseases Antibodies  >83 publications with GNE and Muscular Diseases
 Myositis, Inclusion Body Antibodies  >44 publications with GNE and Myositis, Inclusion Body
 Neurologic Manifestations Antibodies  >13 publications with GNE and Neurologic Manifestations
 Brain Diseases Antibodies  >7 publications with GNE and Brain Diseases
 Neoplasms Antibodies  >7 publications with GNE and Neoplasms
 Inflammation Antibodies  >5 publications with GNE and Inflammation
 Sialic Acid Storage Disease Antibodies  >5 publications with GNE and Sialic Acid Storage Disease
 Proteinuria Antibodies  >4 publications with GNE and Proteinuria
 Drug Toxicity Antibodies  >4 publications with GNE and Drug Toxicity
 Cardiovascular Diseases Antibodies  >3 publications with GNE and Cardiovascular Diseases
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >88 publications with GNE and Muscle
 Brain Antibodies  >13 publications with GNE and Brain
 Embryonic Tissue Antibodies  >8 publications with GNE and Embryonic Tissue
 Kidney Antibodies  >7 publications with GNE and Kidney
 Liver Antibodies  >5 publications with GNE and Liver
 Lung Antibodies  >4 publications with GNE and Lung
 Connective Tissue Antibodies  >3 publications with GNE and Connective Tissue
 Placenta Antibodies  >2 publications with GNE and Placenta
 Prostate Antibodies  >1 publications with GNE and Prostate
 Stomach Antibodies  >1 publications with GNE and Stomach
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