Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow GLS rightarrow Human GLS  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • SDF-1 ELISA Kit
 • Mullerian Inhibiting Substance-Anti Mullerian Hormone (MIS-A... ELISA Kit
 • PIICP ELISA Kit
 • p63 Antibody
 • B-Cell CLL-Lymphoma 2 Like Protein 2 (Bcl2L2) Recombinant Protein
 • GDNF family receptor alpha-like (C6orf144) Recombinant Protein
 • CA72-4 Cancer Antigen Cancer Marker
 • Peptidyl-prolyl cis-trans isomerase A (PPIA) ELISA Kit
 • Benzoylecgonine Antibody
 • Krebs yon den Lundgen-6 (KL-6) ELISA Kit
 • FKBP1A Antibody
 • Inhibin-Alpha A Chain (INHA) Recombinant Protein
 • ACTH Antibody
 • Matrix Metalloproteinase 8 (MMP-8) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

GLS elisa kit :: Human glutaminase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2602981
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $260 +1 FREE 8GB USB
96-Strip-Wells  /  $405 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

glutaminase (GLS), ELISA Kit

★Popular Item★
 Also Known As   

Human Glutaminase kidney isoform, mitochondrial, GLS ELISA Kit

 Product Gene Name   

GLS elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    138280
 3D Structure    ModBase 3D Structure for O94925
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    No cross-reaction with other factors.
Section Bar
 Samples    Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant GLS concentration.
 Assay Type    Sandwich
 Detection Range    10 ng/ml-0.156ng/ml
 Sensitivity    0.05ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GLS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GLS purchase    MBS2602981 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the glutaminase (GLS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 10 ng/ml-0.156ng/ml in biological research samples containing GLS, with an estimated sensitivity of 0.05ng/ml. The ELISA analytical biochemical technique of the MBS2602981 kit is based on GLS antibody-GLS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GLS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GLS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant GLS concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for GLS elisa kit

   Principle of the Assay||This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human GLS monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
Section Bar
 Typical Testing Data/Standard Curve of GLS elisa kit    GLS elisa kit Typical Testing Data/Standard Curve image
Table BarTOPTable Bar
Sample Manual Insert of MBS2602981. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for GLS. It may not necessarily be applicable to this product.
 NCBI GI #    373251164
 NCBI GeneID    2744
 NCBI Accession #    NP_001243239.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001256310.1 [Other Products]
Section Bar
 UniProt Primary Accession #    O94925 [Other Products]
 UniProt Secondary Accession #    Q9UL05; Q9UL06; Q9UL07; Q9UN40 [Other Products]
 UniProt Related Accession #    O94925 [Other Products]
 Molecular Weight    65,460 Da [Similar Products]
Table BarTOPTable Bar
 NCBI Official Full Name    glutaminase kidney isoform, mitochondrial isoform 2
 NCBI Official Synonym Full Names    glutaminase
 NCBI Official Symbol    GLS [Similar Products]
 NCBI Official Synonym Symbols   
GAC; GAM; KGA; GLS1; AAD20
[Similar Products]
 NCBI Protein Information    glutaminase kidney isoform, mitochondrial; K-glutaminase; glutaminase C; L-glutamine amidohydrolase; glutaminase, phosphate-activated
 UniProt Protein Name    Glutaminase kidney isoform, mitochondrial
 UniProt Synonym Protein Names   
K-glutaminase; L-glutamine amidohydrolase
 Protein Family    Glutaminase
 UniProt Gene Name    GLS [Similar Products]
 UniProt Synonym Gene Names    GLS1; KIAA0838; GLS [Similar Products]
 UniProt Entry Name    GLSK_HUMAN
Section Bar
 NCBI Summary for GLS    This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
Section Bar
 UniProt Comments for GLS    glutaminase: Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity. Belongs to the glutaminase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; EC 3.5.1.2; Hydrolase; Other Amino Acids Metabolism - D-Glutamine and D-glutamate; Energy Metabolism - nitrogen; Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 2q32-q34

Cellular Component: mitochondrion; mitochondrial matrix; cytosol

Molecular Function: protein binding; glutaminase activity

Biological Process: synaptic transmission; glutamate secretion; suckling behavior; neurotransmitter secretion; glutamine catabolic process; neurological control of breathing; glutamate biosynthetic process; amino acid biosynthetic process; protein homotetramerization
Table BarTOPTable Bar
 Research Articles on GLS    1. The rate of Glu decarboxylation into GABA by Glnase is an order of magnitude lower than that of Glutamate decarboxylase. Potential impact on the mechanistic aspects of Gln-Glu shuttle in neuroscience and glutaminolysis in tumors, is discussed.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with GLS elisa kitDiseases associated with GLS elisa kit
 Products by Pathway  Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies  Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 D-Glutamine And D-glutamate Metabolism Pathway antibodies  D-Glutamine And D-glutamate Metabolism Pathway Diagram
 D-Glutamine And D-glutamate Metabolism Pathway antibodies  D-Glutamine And D-glutamate Metabolism Pathway Diagram
 GABAergic Synapse Pathway antibodies  GABAergic Synapse Pathway Diagram
 GABAergic Synapse Pathway antibodies  GABAergic Synapse Pathway Diagram
 Glutamate Neurotransmitter Release Cycle Pathway antibodies  Glutamate Neurotransmitter Release Cycle Pathway Diagram
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >46 publications with GLS and Cardiovascular Diseases
 Neoplasms Antibodies  >39 publications with GLS and Neoplasms
 Myocardial Ischemia Antibodies  >15 publications with GLS and Myocardial Ischemia
 Nervous System Diseases Antibodies  >13 publications with GLS and Nervous System Diseases
 Carcinoma Antibodies  >12 publications with GLS and Carcinoma
 Necrosis Antibodies  >11 publications with GLS and Necrosis
 Gastrointestinal Diseases Antibodies  >10 publications with GLS and Gastrointestinal Diseases
 Central Nervous System Diseases Antibodies  >9 publications with GLS and Central Nervous System Diseases
 Brain Diseases Antibodies  >9 publications with GLS and Brain Diseases
 Cardiomyopathies Antibodies  >9 publications with GLS and Cardiomyopathies
Organs/Tissues associated with GLS elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >23 publications with GLS and Brain
 Testis Antibodies  >2 publications with GLS and Testis
 Pancreas Antibodies  >1 publications with GLS and Pancreas
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions