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GPX3 elisa kit :: Human glutathione peroxidase 3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS704704
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
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 Product Name   

glutathione peroxidase 3 (GPX3), ELISA Kit

★Popular Item★
 Also Known As   

Human Glutathione peroxidase 3, GPX3 ELISA Kit

 Product Synonym Names    Human Glutathione peroxidase 3 (GPX3) ELISA kit; GPx-P; GSHPx-3; GSHPx-P; extracellular glutathione peroxidase; glutathione peroxidase 3; glutathione peroxidase 3
 Product Gene Name   

GPX3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS704704 Testing Data    Testing Data PDF
 OMIM    138321
 3D Structure    ModBase 3D Structure for P22352
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human GPX3. No significant cross-reactivity or interference between human GPX3 and analogues was observed.
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 Samples    Serum, plasma and tissue homogenates
 Assay Type    Sandwich
 Detection Range    15.6 uIU/ml -1000 uIU/ml.
 Sensitivity    The minimum detectable dose of human GPX3 is typically less than 3.9uIU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GPX3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GPX3 purchase    MBS704704 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the glutathione peroxidase 3 (GPX3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 uIU/ml -1000 uIU/ml in biological research samples containing GPX3, with an estimated sensitivity of The minimum detectable dose of human GPX3 is typically less than 3.9uIU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS704704 kit is based on GPX3 antibody-GPX3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPX3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GPX3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GPX3 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GPX3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GPX3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPX3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPX3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of GPX3 elisa kit    GPX3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS704704. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GPX3. It may not necessarily be applicable to this product.
 NCBI GI #    6006001
 NCBI GeneID    2878
 NCBI Accession #    NP_002075.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002084.3 [Other Products]
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 UniProt Primary Accession #    P22352 [Other Products]
 UniProt Secondary Accession #    O43787; Q86W78; Q9NZ74; Q9UEL1 [Other Products]
 UniProt Related Accession #    P22352 [Other Products]
 Molecular Weight    25,552 Da
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 NCBI Official Full Name    glutathione peroxidase 3
 NCBI Official Synonym Full Names    glutathione peroxidase 3 (plasma)
 NCBI Official Symbol    GPX3 [Similar Products]
 NCBI Official Synonym Symbols   
GPx-P; GSHPx-3; GSHPx-P
[Similar Products]
 NCBI Protein Information    glutathione peroxidase 3; GPx-3; plasma glutathione peroxidase; extracellular glutathione peroxidase
 UniProt Protein Name    Glutathione peroxidase 3
 UniProt Synonym Protein Names   
Extracellular glutathione peroxidase; Plasma glutathione peroxidase; GPx-P; GSHPx-P
 Protein Family    Glutathione peroxidase
 UniProt Gene Name    GPX3 [Similar Products]
 UniProt Synonym Gene Names    GPXP; GPx-3; GSHPx-3; GPx-P; GSHPx-P [Similar Products]
 UniProt Entry Name    GPX3_HUMAN
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 NCBI Summary for GPX3    This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]
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 UniProt Comments for GPX3    Function: Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione.

Catalytic activity: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O.
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 Research Articles on GPX3    1. APOA1 and GPX3 mRNA levels on qRT-PCR effectively differentiate ovarian from breast carcinoma.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GPX3 elisa kitPathways associated with GPX3 elisa kit
 Reference Product  PubMed Publications
 GSTM3 elisa kit  >4 publications with GPX3 and GSTM3
 GSR elisa kit  >4 publications with GPX3 and GSR
 GSTO1 elisa kit  >2 publications with GPX3 and GSTO1
 GSTA4 elisa kit  >2 publications with GPX3 and GSTA4
 GSTM5 elisa kit  >1 publications with GPX3 and GSTM5
 GSTM2 elisa kit  >1 publications with GPX3 and GSTM2
 GSTA3 elisa kit  >1 publications with GPX3 and GSTA3
 Products by Pathway  Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Detoxification Of Reactive Oxygen Species Pathway antibodies  Detoxification Of Reactive Oxygen Species Pathway Diagram
 Folate Metabolism Pathway antibodies  Folate Metabolism Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Oxidative Stress Pathway antibodies  Oxidative Stress Pathway Diagram
 Selenium Metabolism And Selenoproteins Pathway antibodies  Selenium Metabolism And Selenoproteins Pathway Diagram
Diseases associated with GPX3 elisa kitOrgans/Tissues associated with GPX3 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >26 publications with GPX3 and Cardiovascular Diseases
 Inflammation Antibodies  >22 publications with GPX3 and Inflammation
 Disease Models, Animal Antibodies  >18 publications with GPX3 and Disease Models, Animal
 Nervous System Diseases Antibodies  >17 publications with GPX3 and Nervous System Diseases
 Necrosis Antibodies  >14 publications with GPX3 and Necrosis
 Adenocarcinoma Antibodies  >14 publications with GPX3 and Adenocarcinoma
 Obesity Antibodies  >10 publications with GPX3 and Obesity
 Prostatic Neoplasms Antibodies  >10 publications with GPX3 and Prostatic Neoplasms
 Insulin Resistance Antibodies  >8 publications with GPX3 and Insulin Resistance
 Lung Diseases Antibodies  >7 publications with GPX3 and Lung Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >69 publications with GPX3 and Blood
 Liver Antibodies  >26 publications with GPX3 and Liver
 Kidney Antibodies  >24 publications with GPX3 and Kidney
 Muscle Antibodies  >19 publications with GPX3 and Muscle
 Lung Antibodies  >15 publications with GPX3 and Lung
 Thyroid Antibodies  >14 publications with GPX3 and Thyroid
 Heart Antibodies  >13 publications with GPX3 and Heart
 Brain Antibodies  >9 publications with GPX3 and Brain
 Prostate Antibodies  >9 publications with GPX3 and Prostate
 Adipose Tissue Antibodies  >8 publications with GPX3 and Adipose Tissue
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