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GOLPH3 elisa kit :: Human golgi phosphoprotein 3 (coat-protein) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS916579
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

golgi phosphoprotein 3 (coat-protein) (GOLPH3), ELISA Kit

 Also Known As   

Human Golgi phosphoprotein 3, GOLPH3 ELISA Kit

 Product Synonym Names    Human Golgi phosphoprotein 3 (GOLPH3) ELISA kit; FLJ90675; GOPP1; GPP34; MIDAS; coat-protein; golgi peripheral membrane protein 1; 34 kDa; golgi phosphoprotein 3; golgi protein; golgi-associated protein; golgi phosphoprotein 3 (coat-protein)
 Product Gene Name   

GOLPH3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS916579 Testing Data    Testing Data PDF
 OMIM    612207
 3D Structure    ModBase 3D Structure for Q9H4A6
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human GOLPH3. No significant cross-reactivity or interference between human GOLPH3 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    34.37 pg/ml -2200 pg/ml.
 Sensitivity    The minimum detectable dose of human GOLPH3 is typically less than 8.6 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GOLPH3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GOLPH3 purchase    MBS916579 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the golgi phosphoprotein 3 (coat-protein) (GOLPH3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 34.37 pg/ml -2200 pg/ml in biological research samples containing GOLPH3, with an estimated sensitivity of The minimum detectable dose of human GOLPH3 is typically less than 8.6 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS916579 kit is based on GOLPH3 antibody-GOLPH3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GOLPH3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GOLPH3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for GOLPH3 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GOLPH3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GOLPH3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GOLPH3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GOLPH3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of GOLPH3 elisa kit    GOLPH3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS916579. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GOLPH3. It may not necessarily be applicable to this product.
 NCBI GI #    11545859
 NCBI GeneID    64083
 NCBI Accession #    NP_071413.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_022130.3 [Other Products]
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 UniProt Primary Accession #    Q9H4A6 [Other Products]
 UniProt Secondary Accession #    Q9UIW5 [Other Products]
 UniProt Related Accession #    Q9H4A6 [Other Products]
 Molecular Weight    33,811 Da
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 NCBI Official Full Name    Golgi phosphoprotein 3
 NCBI Official Synonym Full Names    golgi phosphoprotein 3 (coat-protein)
 NCBI Official Symbol    GOLPH3 [Similar Products]
 NCBI Official Synonym Symbols   
GOPP1; GPP34; MIDAS
[Similar Products]
 NCBI Protein Information    Golgi phosphoprotein 3; coat-protein; golgi protein; coat protein GPP34; golgi-associated protein; mitochondrial DNA absence factor; golgi peripheral membrane protein 1, 34 kDa
 UniProt Protein Name    Golgi phosphoprotein 3
 UniProt Synonym Protein Names   
Coat protein GPP34; Mitochondrial DNA absence factor; MIDAS
 Protein Family    Golgi phosphoprotein
 UniProt Gene Name    GOLPH3 [Similar Products]
 UniProt Synonym Gene Names    GPP34; MIDAS [Similar Products]
 UniProt Entry Name    GOLP3_HUMAN
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 NCBI Summary for GOLPH3    The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Jul 2008]
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 UniProt Comments for GOLPH3    GOLPH3: Mediates the cis and medial Golgi localization of mannosyltransferases through direct binding of their cytosolic domains. Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene. Belongs to the GOLPH3/VPS74 family.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 5p13.3

Cellular Component: nucleoplasm; Golgi apparatus; Golgi cisterna; mitochondrion; plasma membrane; mitochondrial intermembrane space; trans-Golgi network; cytosol; endosome

Molecular Function: protein binding; enzyme binding

Biological Process: lamellipodium biogenesis; cell migration; glycoprotein biosynthetic process; protein secretion; cell proliferation; positive regulation of protein secretion; Golgi to plasma membrane protein transport; gene expression; Golgi vesicle budding; leukocyte tethering or rolling; Golgi organization and biogenesis; protein retention in Golgi; positive regulation of TOR signaling pathway
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 Research Articles on GOLPH3    1. These results suggest that GOLPH3 expression is likely to have important roles in renal cell carcinoma (RCC) development and progression, and that GOLPH3 is a prognostic biomarker and a promising therapeutic target for RCC.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with GOLPH3 elisa kitDiseases associated with GOLPH3 elisa kit
 Products by Pathway  Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >6 publications with GOLPH3 and Neoplasms
 Cell Transformation, Neoplastic Antibodies  >2 publications with GOLPH3 and Cell Transformation, Neoplastic
 Brain Diseases Antibodies  >1 publications with GOLPH3 and Brain Diseases
 Hepatitis Antibodies  >1 publications with GOLPH3 and Hepatitis
 Mental Disorders Antibodies  >1 publications with GOLPH3 and Mental Disorders
 Neurobehavioral Manifestations Antibodies  >1 publications with GOLPH3 and Neurobehavioral Manifestations
 Learning Disorders Antibodies  >1 publications with GOLPH3 and Learning Disorders
 Genital Diseases, Male Antibodies  >1 publications with GOLPH3 and Genital Diseases, Male
 Prostatic Diseases Antibodies  >1 publications with GOLPH3 and Prostatic Diseases
Organs/Tissues associated with GOLPH3 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >4 publications with GOLPH3 and Lung
 Muscle Antibodies  >2 publications with GOLPH3 and Muscle
 Prostate Antibodies  >2 publications with GOLPH3 and Prostate
 Brain Antibodies  >2 publications with GOLPH3 and Brain
 Vascular Antibodies  >1 publications with GOLPH3 and Vascular
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