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GZMB elisa kit :: Human granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS703753
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $355 +1 FREE 8GB USB
96-Strip-Wells  /  $510 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,180 +2 FREE 8GB USB
10x96-Strip-Wells  /  $4,120 +5 FREE 8GB USB
 
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 Product Name   

granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) (GZMB), ELISA Kit

★Popular Item★
 Also Known As   

Human granzymes B, Gzms-B ELISA Kit

 Product Synonym Names    Human granzymes B; Gzms-B ELISA Kit; CCPI; CGL-1; CGL1; CSP-B; CSPB; CTLA1; CTSGL1; HLP; SECT; T-cell serine protease 1-3E; cathepsin G-like 1; cytotoxic serine protease B; fragmentin 2; granzyme B; granzyme B (granzyme 2; cytotoxic T-lymphocyte-associated serine esterase 1)
 Product Gene Name   

GZMB elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS703753 Testing Data    Testing Data PDF
 OMIM    123910
 3D Structure    ModBase 3D Structure for P10144
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human Gzms-B. No significant cross-reactivity or interference between human Gzms-B and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    1.56 pg/ml -100 pg/ml.
 Sensitivity    The minimum detectable dose of human Gzms-B is typically less than 0.39 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GZMB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GZMB purchase    MBS703753 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) (GZMB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.56 pg/ml -100 pg/ml in biological research samples containing GZMB, with an estimated sensitivity of The minimum detectable dose of human Gzms-B is typically less than 0.39 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS703753 kit is based on GZMB antibody-GZMB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GZMB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GZMB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GZMB elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Gzms-B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Gzms-B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Gzms-B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Gzms-B bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of GZMB elisa kit    GZMB elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS703753. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GZMB. It may not necessarily be applicable to this product.
 NCBI GI #    221625528
 NCBI GeneID    3002
 NCBI Accession #    NP_004122.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_004131.4 [Other Products]
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 UniProt Primary Accession #    P10144 [Other Products]
 UniProt Secondary Accession #    Q8N1D2; Q9UCC1 [Other Products]
 UniProt Related Accession #    P10144 [Other Products]
 Molecular Weight    27,716 Da
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 NCBI Official Full Name    granzyme B
 NCBI Official Synonym Full Names    granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
 NCBI Official Symbol    GZMB [Similar Products]
 NCBI Official Synonym Symbols   
HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
[Similar Products]
 NCBI Protein Information    granzyme B; C11; CTLA-1; fragmentin 2; fragmentin-2; cathepsin G-like 1; human lymphocyte protein; T-cell serine protease 1-3E; cytotoxic serine protease B; cytotoxic T-lymphocyte proteinase 2
 UniProt Protein Name    Granzyme B
 UniProt Synonym Protein Names   
C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; HLP; SECT; T-cell serine protease 1-3E
 Protein Family    Granzyme
 UniProt Gene Name    GZMB [Similar Products]
 UniProt Synonym Gene Names    CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP [Similar Products]
 UniProt Entry Name    GRAB_HUMAN
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 NCBI Summary for GZMB    Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq, Jul 2008]
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 UniProt Comments for GZMB    Function: This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

Catalytic activity: Preferential cleavage: -Asp-|-Xaa- >> -Asn-|-Xaa- > -Met-|-Xaa-, -Ser-|-Xaa-.1 PublicationManual assertion based on experiment in:Ref.12

Enzyme regulation: Inactivated by the serine protease inhibitor diisopropylfluorophosphate.1 PublicationManual assertion based on experiment in:Ref.12
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 Research Articles on GZMB    1. These data indicate that IBD-related inflammation is marked by mucosal accumulation of cytotoxic, GrB-expressing CD19(+) and IgA(+) cells, suggesting a role for these cells in IBD-associated epithelial damage.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GZMB elisa kitPathways associated with GZMB elisa kit
 Reference Product  PubMed Publications
 PRF1 elisa kit  >1244 publications with GZMB and PRF1
 CASP3 elisa kit  >141 publications with GZMB and CASP3
 BID elisa kit  >57 publications with GZMB and BID
 SERPINB9 elisa kit  >57 publications with GZMB and SERPINB9
 IL2RA elisa kit  >48 publications with GZMB and IL2RA
 CASP7 elisa kit  >19 publications with GZMB and CASP7
 IL2RB elisa kit  >14 publications with GZMB and IL2RB
 IGF2R elisa kit  >13 publications with GZMB and IGF2R
 IL2 elisa kit  >6 publications with GZMB and IL2
 Products by Pathway  Pathway Diagram
 Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway antibodies  Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Downstream Signaling In Naive CD8+ T Cells Pathway antibodies  Downstream Signaling In Naive CD8+ T Cells Pathway Diagram
Diseases associated with GZMB elisa kitOrgans/Tissues associated with GZMB elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >303 publications with GZMB and Necrosis
 Inflammation Antibodies  >192 publications with GZMB and Inflammation
 Disease Models, Animal Antibodies  >112 publications with GZMB and Disease Models, Animal
 Nervous System Diseases Antibodies  >95 publications with GZMB and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >95 publications with GZMB and Neoplasms, Experimental
 Liver Diseases Antibodies  >74 publications with GZMB and Liver Diseases
 Hepatitis Antibodies  >54 publications with GZMB and Hepatitis
 Brain Diseases Antibodies  >35 publications with GZMB and Brain Diseases
 Breast Neoplasms Antibodies  >33 publications with GZMB and Breast Neoplasms
 Kidney Diseases Antibodies  >27 publications with GZMB and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >806 publications with GZMB and Blood
 Kidney Antibodies  >80 publications with GZMB and Kidney
 Lung Antibodies  >62 publications with GZMB and Lung
 Liver Antibodies  >61 publications with GZMB and Liver
 Spleen Antibodies  >56 publications with GZMB and Spleen
 Bone Marrow Antibodies  >44 publications with GZMB and Bone Marrow
 Heart Antibodies  >32 publications with GZMB and Heart
 Intestine Antibodies  >28 publications with GZMB and Intestine
 Pancreas Antibodies  >13 publications with GZMB and Pancreas
 Stomach Antibodies  >10 publications with GZMB and Stomach
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