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HES1 elisa kit :: Human hairy and enhancer of split 1, (Drosophila) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS910338
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

hairy and enhancer of split 1, (Drosophila) (HES1), ELISA Kit

★Popular Item★
 Also Known As   

Human Transcription factor HES-1, HES1 ELISA Kit

 Product Synonym Names    Human Transcription factor HES-1 (HES1) ELISA kit; FLJ20408; HES-1; HHL; HRY; bHLHb39; hairy and enhancer of split 1; hairy homolog; transcription factor HES-1; hairy and enhancer of split 1; (Drosophila)
 Product Gene Name   

HES1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS910338 Testing Data    Testing Data PDF
 OMIM    139605
 3D Structure    ModBase 3D Structure for Q14469
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human HES1. No significant cross-reactivity or interference between human HES1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    28 pg/ml -1800 pg/ml.
 Sensitivity    The minimum detectable dose of human HES1 is typically less than 7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HES1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HES1 purchase    MBS910338 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the hairy and enhancer of split 1, (Drosophila) (HES1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 28 pg/ml -1800 pg/ml in biological research samples containing HES1, with an estimated sensitivity of The minimum detectable dose of human HES1 is typically less than 7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS910338 kit is based on HES1 antibody-HES1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HES1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HES1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HES1 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HES1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HES1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HES1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HES1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of HES1 elisa kit    HES1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS910338. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HES1. It may not necessarily be applicable to this product.
 NCBI GI #    5031763
 NCBI GeneID    3280
 NCBI Accession #    NP_005515.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_005524.3 [Other Products]
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 UniProt Primary Accession #    Q14469 [Other Products]
 UniProt Secondary Accession #    Q6FHB2 [Other Products]
 UniProt Related Accession #    Q14469 [Other Products]
 Molecular Weight    29,541 Da
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 NCBI Official Full Name    transcription factor HES-1
 NCBI Official Synonym Full Names    hes family bHLH transcription factor 1
 NCBI Official Symbol    HES1 [Similar Products]
 NCBI Official Synonym Symbols   
HHL; HRY; HES-1; bHLHb39
[Similar Products]
 NCBI Protein Information    transcription factor HES-1; hairy homolog; hairy-like protein; class B basic helix-loop-helix protein 39
 UniProt Protein Name    Transcription factor HES-1
 UniProt Synonym Protein Names   
Class B basic helix-loop-helix protein 39; bHLHb39; Hairy and enhancer of split 1; Hairy homolog; Hairy-like protein; hHL
 Protein Family    Protein
 UniProt Gene Name    HES1 [Similar Products]
 UniProt Synonym Gene Names    BHLHB39; HL; HRY; bHLHb39; hHL [Similar Products]
 UniProt Entry Name    HES1_HUMAN
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 NCBI Summary for HES1    This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. [provided by RefSeq, Jul 2008]
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 UniProt Comments for HES1    HES1: Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage.

Protein type: Cell development/differentiation; Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q28-q29

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; chaperone binding; histone deacetylase binding; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; radial glial cell differentiation in the forebrain; hindbrain morphogenesis; midbrain-hindbrain boundary morphogenesis; somatic stem cell maintenance; regulation of fat cell differentiation; regulation of timing of neuron differentiation; cell maturation; positive regulation of JAK-STAT cascade; negative regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of cell proliferation; midbrain development; adenohypophysis development; positive regulation of T cell proliferation; protein complex assembly; trochlear nerve development; positive regulation of mitotic cell cycle, embryonic; oculomotor nerve development; cell adhesion; positive regulation of BMP signaling pathway; positive regulation of Notch signaling pathway; positive regulation of astrocyte differentiation; STAT protein nuclear translocation; positive regulation of DNA binding; nervous system development; cell migration; Notch signaling pathway; smoothened signaling pathway; auditory receptor cell fate determination; thymus development; liver development; endocrine pancreas development; negative regulation of oligodendrocyte differentiation; telencephalon development; lateral inhibition; negative regulation of auditory receptor cell differentiation; neuron morphogenesis during differentiation; artery morphogenesis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; lung development
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 Research Articles on HES1    1. Notch signaling can directly downregulate MUC5AC promoter activity through Hes1-dependent mechanisms.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HES1 elisa kitPathways associated with HES1 elisa kit
 Reference Product  PubMed Publications
 NOTCH1 elisa kit  >414 publications with HES1 and NOTCH1
 ASCL1 elisa kit  >58 publications with HES1 and ASCL1
 RBPJ elisa kit  >52 publications with HES1 and RBPJ
 SHH elisa kit  >23 publications with HES1 and SHH
 NEUROG3 elisa kit  >23 publications with HES1 and NEUROG3
 TLE1 elisa kit  >19 publications with HES1 and TLE1
 FANCD2 elisa kit  >3 publications with HES1 and FANCD2
 FANCA elisa kit  >2 publications with HES1 and FANCA
 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 AhR Pathway antibodies  AhR Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway Diagram
 Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway Diagram
 Delta-Notch Signaling Pathway antibodies  Delta-Notch Signaling Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 FBXW7 Mutants And NOTCH1 In Cancer Pathway antibodies  FBXW7 Mutants And NOTCH1 In Cancer Pathway Diagram
 Fanconi Anemia Pathway antibodies  Fanconi Anemia Pathway Diagram
Diseases associated with HES1 elisa kitOrgans/Tissues associated with HES1 elisa kit
 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >59 publications with HES1 and Adenocarcinoma
 Liver Diseases Antibodies  >30 publications with HES1 and Liver Diseases
 Necrosis Antibodies  >28 publications with HES1 and Necrosis
 Inflammation Antibodies  >28 publications with HES1 and Inflammation
 Lung Neoplasms Antibodies  >26 publications with HES1 and Lung Neoplasms
 Neuroectodermal Tumors, Primitive Antibodies  >23 publications with HES1 and Neuroectodermal Tumors, Primitive
 Neoplasms, Experimental Antibodies  >19 publications with HES1 and Neoplasms, Experimental
 Carcinoma, Hepatocellular Antibodies  >17 publications with HES1 and Carcinoma, Hepatocellular
 Carcinoma, Non-Small-Cell Lung Antibodies  >16 publications with HES1 and Carcinoma, Non-Small-Cell Lung
 Liver Neoplasms Antibodies  >16 publications with HES1 and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >191 publications with HES1 and Brain
 Nerve Antibodies  >127 publications with HES1 and Nerve
 Embryonic Tissue Antibodies  >113 publications with HES1 and Embryonic Tissue
 Blood Antibodies  >82 publications with HES1 and Blood
 Bone Antibodies  >78 publications with HES1 and Bone
 Muscle Antibodies  >54 publications with HES1 and Muscle
 Pancreas Antibodies  >50 publications with HES1 and Pancreas
 Eye Antibodies  >47 publications with HES1 and Eye
 Lung Antibodies  >39 publications with HES1 and Lung
 Liver Antibodies  >36 publications with HES1 and Liver
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