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HGF elisa kit :: Human hepatocyte growth factor (hepapoietin A; scatter factor) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS705107
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $375 +1 FREE 8GB USB
96-Strip-Wells  /  $535 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,295 +2 FREE 8GB USB
10x96-Strip-Wells  /  $4,330 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), ELISA Kit

★Popular Item★
 Also Known As   

Human hepatocyte growth factor, HGF ELISA Kit

 Product Synonym Names    Human hepatocyte growth factor; HGF ELISA kit; DFNB39; F-TCF; HGFB; HPTA; SF; OTTHUMP00000206710; fibroblast-derived tumor cytotoxic factor; hepatocyte growth factor; lung fibroblast-derived mitogen; hepatocyte growth factor (hepapoietin A; scatter factor)
 Product Gene Name   

HGF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS705107 Testing Data    Testing Data PDF
 OMIM    gene 608265
 3D Structure    ModBase 3D Structure for P14210
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human HGF. No significant cross-reactivity or interference between Human HGF and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Detection Range    6.25 pg/ml-400 pg/ml
 Sensitivity    1.56 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HGF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HGF purchase    MBS705107 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the hepatocyte growth factor (hepapoietin A; scatter factor) (HGF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6.25 pg/ml-400 pg/ml in biological research samples containing HGF, with an estimated sensitivity of 1.56 pg/ml. The ELISA analytical biochemical technique of the MBS705107 kit is based on HGF antibody-HGF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HGF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HGF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HGF elisa kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for HGF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain HGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of HGF elisa kit    HGF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS705107. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HGF. It may not necessarily be applicable to this product.
 NCBI GI #    33859835
 NCBI GeneID    3082
 NCBI Accession #    NP_000592.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_000601.4 [Other Products]
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 UniProt Primary Accession #    P14210 [Other Products]
 UniProt Secondary Accession #    Q02935; Q13494; Q14519; Q3KRB2; Q8TCE2; Q9BYL9; Q9BYM0; Q9UDU6; A1L3U6 [Other Products]
 UniProt Related Accession #    P14210 [Other Products]
 Molecular Weight    83,134 Da
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 NCBI Official Full Name    hepatocyte growth factor isoform 1 preproprotein
 NCBI Official Synonym Full Names    hepatocyte growth factor (hepapoietin A; scatter factor)
 NCBI Official Symbol    HGF [Similar Products]
 NCBI Official Synonym Symbols   
SF; HGFB; HPTA; F-TCF; DFNB39
[Similar Products]
 NCBI Protein Information    hepatocyte growth factor; hepatopoeitin-A; hepatopoietin-A; lung fibroblast-derived mitogen; fibroblast-derived tumor cytotoxic factor
 UniProt Protein Name    Hepatocyte growth factor
 UniProt Synonym Protein Names   
Hepatopoietin-A; Scatter factor; SF
 Protein Family    Hepatocyte growth factor
 UniProt Gene Name    HGF [Similar Products]
 UniProt Synonym Gene Names    HPTA; SF [Similar Products]
 UniProt Entry Name    HGF_HUMAN
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 NCBI Summary for HGF    Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
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 UniProt Comments for HGF    Function: Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.2 PublicationsManual assertion based on experiment in:Ref.23
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 Research Articles on HGF    1. HGF may be a potential prognostic marker in HPV-negative tonsillar squamous cell carcinoma
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HGF elisa kitPathways associated with HGF elisa kit
 Reference Product  PubMed Publications
 MET elisa kit  >2220 publications with HGF and MET
 ST14 elisa kit  >8 publications with HGF and ST14
 SDC1 elisa kit  >6 publications with HGF and SDC1
 NRP1 elisa kit  >5 publications with HGF and NRP1
 LCN2 elisa kit  >3 publications with HGF and LCN2
 FN1 elisa kit  >2 publications with HGF and FN1
 PLAU elisa kit  >1 publications with HGF and PLAU
 HGFAC elisa kit  >1 publications with HGF and HGFAC
 F11 elisa kit  >1 publications with HGF and F11
 Products by Pathway  Pathway Diagram
 Arf6 Signaling Events Pathway antibodies  Arf6 Signaling Events Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 FGF Signaling Pathway antibodies  FGF Signaling Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
Diseases associated with HGF elisa kitOrgans/Tissues associated with HGF elisa kit
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >731 publications with HGF and Liver Diseases
 Vascular Diseases Antibodies  >586 publications with HGF and Vascular Diseases
 Cardiovascular Diseases Antibodies  >569 publications with HGF and Cardiovascular Diseases
 Necrosis Antibodies  >443 publications with HGF and Necrosis
 Fibrosis Antibodies  >422 publications with HGF and Fibrosis
 Liver Neoplasms Antibodies  >420 publications with HGF and Liver Neoplasms
 Neoplasm Invasiveness Antibodies  >415 publications with HGF and Neoplasm Invasiveness
 Inflammation Antibodies  >394 publications with HGF and Inflammation
 Kidney Diseases Antibodies  >294 publications with HGF and Kidney Diseases
 Neoplasms, Experimental Antibodies  >292 publications with HGF and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1506 publications with HGF and Blood
 Liver Antibodies  >1431 publications with HGF and Liver
 Vascular Antibodies  >970 publications with HGF and Vascular
 Kidney Antibodies  >670 publications with HGF and Kidney
 Bone Antibodies  >586 publications with HGF and Bone
 Lung Antibodies  >527 publications with HGF and Lung
 Muscle Antibodies  >474 publications with HGF and Muscle
 Bone Marrow Antibodies  >406 publications with HGF and Bone Marrow
 Embryonic Tissue Antibodies  >322 publications with HGF and Embryonic Tissue
 Brain Antibodies  >264 publications with HGF and Brain
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