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HIST1H3A elisa kit :: Human histone cluster 1, H3a ELISA Kit

Scan QR to view Datasheet Catalog #    MBS937951
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

histone cluster 1, H3a (HIST1H3A), ELISA Kit

★Popular Item★
 Also Known As   

Human Histone H3.1, HIST1H3A ELISA Kit

 Product Synonym Names    Human Histone H3.1 (HIST1H3A) ELISA kit; H3/A; H3FA; H3 histone family; member A; histone 1; H3a; histone cluster 1; H3a
 Product Gene Name   

HIST1H3A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS937951 Testing Data    Testing Data PDF
 OMIM    gene 602819
 3D Structure    ModBase 3D Structure for P68431
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human HIST1H3A. No significant cross-reactivity or interference between human HIST1H3A and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates
 Detection Range    23.5 pg/ml -1500 pg/mL
 Sensitivity    <5.8 pg/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HIST1H3A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HIST1H3A purchase    MBS937951 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the histone cluster 1, H3a (HIST1H3A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 23.5 pg/ml -1500 pg/mL in biological research samples containing HIST1H3A, with an estimated sensitivity of <5.8 pg/mL. The ELISA analytical biochemical technique of the MBS937951 kit is based on HIST1H3A antibody-HIST1H3A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HIST1H3A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HIST1H3A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HIST1H3A elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HIST1H3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HIST1H3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIST1H3A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIST1H3A bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of HIST1H3A elisa kit    HIST1H3A elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS937951. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HIST1H3A. It may not necessarily be applicable to this product.
 NCBI GI #    4504281
 NCBI GeneID    8350
 NCBI Accession #    NP_003520.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_003529.2 [Other Products]
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 UniProt Primary Accession #    P68431 [Other Products]
 UniProt Secondary Accession #    P02295; P02296; P16106; Q6ISV8; Q6NWP8; Q6NWP9; Q6NXU4; Q71DJ3; Q93081; A0PJT7; A5PLR1 [Other Products]
 UniProt Related Accession #    P68431 [Other Products]
 Molecular Weight    15,404 Da
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 NCBI Official Full Name    histone H3.1
 NCBI Official Synonym Full Names    histone cluster 1, H3a
 NCBI Official Symbol    HIST1H3A [Similar Products]
 NCBI Official Synonym Symbols   
H3/A; H3FA
[Similar Products]
 NCBI Protein Information    histone H3.1; histone H3/a; histone 1, H3a; H3 histone family, member A
 UniProt Protein Name    Histone H3.1
 UniProt Synonym Protein Names   
Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l
 UniProt Gene Name    HIST1H3A [Similar Products]
 UniProt Synonym Gene Names    H3FA [Similar Products]
 UniProt Entry Name    H31_HUMAN
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 NCBI Summary for HIST1H3A    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
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 UniProt Comments for HIST1H3A    H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p22.2

Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus

Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity

Biological Process: chromatin silencing at rDNA; nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; blood coagulation; DNA replication-dependent nucleosome assembly; DNA methylation on cytosine; regulation of gene expression, epigenetic
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 Research Articles on HIST1H3A    1. The authors report that de novo CENP-A assembly and kinetochore formation on human centromeric alphoid DNA arrays is regulated by a histone H3K9 acetyl/methyl balance.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with HIST1H3A elisa kitOrgans/Tissues associated with HIST1H3A elisa kit
 Products by Pathway  Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Amyloids Pathway antibodies  Amyloids Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Factors Involved In Megakaryocyte Development And Platelet Production Pathway antibodies  Factors Involved In Megakaryocyte Development And Platelet Production Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Meiosis Pathway antibodies  Meiosis Pathway Diagram
 Meiotic Recombination Pathway antibodies  Meiotic Recombination Pathway Diagram
 RNA Polymerase I Chain Elongation Pathway antibodies  RNA Polymerase I Chain Elongation Pathway Diagram
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >2 publications with HIST1H3A and Brain
 Blood Antibodies  >1 publications with HIST1H3A and Blood
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