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HAP1 elisa kit :: Human huntingtin-associated protein 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS922159
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

huntingtin-associated protein 1 (HAP1), ELISA Kit

 Also Known As   

Human Huntingtin-associated protein 1, HAP1 ELISA Kit

 Product Synonym Names    Human Huntingtin-associated protein 1 (HAP1) ELISA kit; HAP2; HIP5; HLP; hHLP1; huntingtin-associated protein 2; neuroan 1; huntingtin-associated protein 1
 Product Gene Name   

HAP1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 MBS922159 Testing Data    Testing Data PDF
 OMIM    600947
 3D Structure    ModBase 3D Structure for P54257
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human HAP1. No significant cross-reactivity or interference between human HAP1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates.
 Assay Type    Sandwich (Quantitative)
 Detection Range    62.5 pg/ml -4000 pg/ml.
 Sensitivity    15.6 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of HAP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HAP1 purchase    MBS922159 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the huntingtin-associated protein 1 (HAP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5 pg/ml -4000 pg/ml in biological research samples containing HAP1, with an estimated sensitivity of 15.6 pg/ml. The ELISA analytical biochemical technique of the MBS922159 kit is based on HAP1 antibody-HAP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HAP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HAP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for HAP1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HAP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of HAP1 elisa kit    HAP1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS922159
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NCBI/Uniprot data below describe general gene information for HAP1. It may not necessarily be applicable to this product.
 NCBI GI #    120431743
 NCBI GeneID    9001
 NCBI Accession #    NP_001073339.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001079870.1 [Other Products]
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 UniProt Primary Accession #    P54257 [Other Products]
 UniProt Secondary Accession #    O75358; Q59GK4; Q9H4G3; Q9HA98; Q9NY90; A8MQB5 [Other Products]
 UniProt Related Accession #    P54257 [Other Products]
 Molecular Weight    75,506 Da
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 NCBI Official Full Name    huntingtin-associated protein 1 isoform 3
 NCBI Official Synonym Full Names    huntingtin-associated protein 1
 NCBI Official Symbol    HAP1 [Similar Products]
 NCBI Official Synonym Symbols   
HLP; HAP2; HIP5; hHLP1
[Similar Products]
 NCBI Protein Information    huntingtin-associated protein 1; HAP-1; neuroan 1; huntingtin-associated protein 2
 UniProt Protein Name    Huntingtin-associated protein 1
 UniProt Synonym Protein Names   
Neuroan 1
 Protein Family    Huntingtin-associated protein
 UniProt Gene Name    HAP1 [Similar Products]
 UniProt Synonym Gene Names    HAP2; HLP1; HAP-1 [Similar Products]
 UniProt Entry Name    HAP1_HUMAN
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 NCBI Summary for HAP1    Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein that interacts with huntingtin, with two cytoskeletal proteins (dynactin and pericentriolar autoantigen protein 1), and with a hepatocyte growth factor-regulated tyrosine kinase substrate. The interactions with cytoskeletal proteins and a kinase substrate suggest a role for this protein in vesicular trafficking or organelle transport. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for HAP1    HAP1: Associates specifically with huntingtin. This binding is enhanced by an expanded polyglutamine repeat. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2-q21.3

Cellular Component: synaptic vesicle; cytoskeleton; mitochondrion; lysosome; endoplasmic reticulum; axon; autophagic vacuole; inclusion body; cell junction; nucleus; actin cytoskeleton

Molecular Function: protein binding; brain-derived neurotrophic factor binding

Biological Process: positive regulation of inositol-1,4,5-triphosphate receptor activity; positive regulation of neurogenesis; exocytosis; nerve growth factor receptor signaling pathway; positive regulation of synaptic transmission, GABAergic; hypothalamus cell differentiation; retrograde axon cargo transport; regulation of exocytosis; synaptic transmission; protein transport; protein localization; cell projection organization and biogenesis; positive regulation of epidermal growth factor receptor signaling pathway; vesicle transport along microtubule; positive regulation of neurotrophin production; autophagy; cerebellum development; brain development; anterograde axon cargo transport
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 Research Articles on HAP1    1. Overexpression of HAP1 reduced in vitro cell growth in breast cancer cell lines.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HAP1 elisa kitPathways associated with HAP1 elisa kit
 Reference Product  PubMed Publications
 HTT elisa kit  >17 publications with HAP1 and HTT
 PCM1 elisa kit  >4 publications with HAP1 and PCM1
 TBP elisa kit  >2 publications with HAP1 and TBP
 Products by Pathway  Pathway Diagram
 GABAergic Synapse Pathway antibodies  GABAergic Synapse Pathway Diagram
 GABAergic Synapse Pathway antibodies  GABAergic Synapse Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
Diseases associated with HAP1 elisa kitOrgans/Tissues associated with HAP1 elisa kit
 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >49 publications with HAP1 and Mental Disorders
 Cognition Disorders Antibodies  >44 publications with HAP1 and Cognition Disorders
 Movement Disorders Antibodies  >44 publications with HAP1 and Movement Disorders
 Death Antibodies  >17 publications with HAP1 and Death
 Disease Models, Animal Antibodies  >12 publications with HAP1 and Disease Models, Animal
 Vascular Diseases Antibodies  >8 publications with HAP1 and Vascular Diseases
 Lung Diseases Antibodies  >7 publications with HAP1 and Lung Diseases
 Lung Neoplasms Antibodies  >6 publications with HAP1 and Lung Neoplasms
 Melanoma Antibodies  >5 publications with HAP1 and Melanoma
 Nerve Degeneration Antibodies  >3 publications with HAP1 and Nerve Degeneration
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >68 publications with HAP1 and Brain
 Lung Antibodies  >11 publications with HAP1 and Lung
 Embryonic Tissue Antibodies  >9 publications with HAP1 and Embryonic Tissue
 Liver Antibodies  >6 publications with HAP1 and Liver
 Pancreas Antibodies  >4 publications with HAP1 and Pancreas
 Kidney Antibodies  >4 publications with HAP1 and Kidney
 Muscle Antibodies  >3 publications with HAP1 and Muscle
 Heart Antibodies  >3 publications with HAP1 and Heart
 Spleen Antibodies  >2 publications with HAP1 and Spleen
 Eye Antibodies  >2 publications with HAP1 and Eye
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