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HADH elisa kit :: Human hydroxyacyl-Coenzyme A dehydrogenase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS920669
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

hydroxyacyl-Coenzyme A dehydrogenase (HADH), ELISA Kit

★Popular Item★
 Also Known As   

Human Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial, HADH ELISA Kit

 Product Synonym Names    Human Hydroxyacyl-coenzyme A dehydrogenase; mitochondrial (HADH) ELISA kit; HADH1; HADHSC; SCHAD; L-3-hydroxyacyl-Coenzyme A dehydrogenase; short chain; hydroxyacyl-Coenzyme A dehydrogenase
 Product Gene Name   

HADH elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS920669 Testing Data    Testing Data PDF
 OMIM    gene 609975
 3D Structure    ModBase 3D Structure for Q16836
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human HADH. No significant cross-reactivity or interference between Human HADH and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    25 pg/ml-1600 pg/ml
 Sensitivity    6.25 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Biosynthesis/Metabolism
 Protein Biological Process 2    Lipogenesis and lipometabolism
 Protein Biological Process 3    Fatty acid metabolism
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HADH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HADH purchase    MBS920669 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the hydroxyacyl-Coenzyme A dehydrogenase (HADH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 25 pg/ml-1600 pg/ml in biological research samples containing HADH, with an estimated sensitivity of 6.25 pg/ml. The ELISA analytical biochemical technique of the MBS920669 kit is based on HADH antibody-HADH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HADH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HADH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for HADH elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HADH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HADH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HADH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HADH bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of HADH elisa kit    HADH elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS920669. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HADH. It may not necessarily be applicable to this product.
 NCBI GI #    296179427
 NCBI GeneID    3033
 NCBI Accession #    NP_001171634.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001184705.2 [Other Products]
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 UniProt Primary Accession #    Q16836 [Other Products]
 UniProt Secondary Accession #    O00324; O00397; O00753; Q4W5B4; J3KQ17 [Other Products]
 UniProt Related Accession #    Q16836 [Other Products]
 Molecular Weight    34,294 Da
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 NCBI Official Full Name    hydroxyacyl-coenzyme A dehydrogenase, mitochondrial isoform 1
 NCBI Official Synonym Full Names    hydroxyacyl-CoA dehydrogenase
 NCBI Official Symbol    HADH [Similar Products]
 NCBI Official Synonym Symbols   
HAD; HCDH; HHF4; HADH1; SCHAD; HADHSC; MSCHAD
[Similar Products]
 NCBI Protein Information    hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; short-chain 3-hydroxyacyl-CoA dehydrogenase; L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain; medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase
 UniProt Protein Name    Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial
 UniProt Synonym Protein Names   
Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase
 Protein Family    Hydroxyacyl-coenzyme A dehydrogenase
 UniProt Gene Name    HADH [Similar Products]
 UniProt Synonym Gene Names    HAD; HADHSC; SCHAD; HCDH [Similar Products]
 UniProt Entry Name    HCDH_HUMAN
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 NCBI Summary for HADH    This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15. [provided by RefSeq, May 2010]
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 UniProt Comments for HADH    HADHSC: Plays an essential role in the mitochondrial beta- oxidation of short chain fatty acids. Exerts it highest activity toward 3-hydroxybutyryl-CoA. Defects in HADH are the cause of 3-alpha-hydroxyacyl-CoA dehydrogenase deficiency (HADH deficiency). HADH deficiency is a metabolic disorder with various clinical presentations including hypoglycemia, hepatoencephalopathy, myopathy or cardiomyopathy, and in some cases sudden death. Defects in HADH are the cause of familial hyperinsulinemic hypoglycemia type 4 (HHF4); also known as persistent hyperinsulinemic hypoglycemia of infancy (PHHI) or congenital hyperinsulinism. HHF is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. It causes nesidioblastosis, a diffuse abnormality of the pancreas in which there is extensive, often disorganized formation of new islets. Unless early and aggressive intervention is undertaken, brain damage from recurrent episodes of hypoglycemia may occur. HHF4 should be easily recognizable by analysis of acylcarnitine species and that this disorder responds well to treatment with diazoxide. It provides the first 'experiment of nature' that links impaired fatty acid oxidation to hyperinsulinism and that provides support for the concept that a lipid signaling pathway is implicated in the control of insulin secretion. Belongs to the 3-hydroxyacyl-CoA dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - tryptophan; Oxidoreductase; Lipid Metabolism - fatty acid elongation in mitochondria; Amino Acid Metabolism - valine, leucine and isoleucine degradation; EC 1.1.1.35; Amino Acid Metabolism - lysine degradation; Carbohydrate Metabolism - butanoate; Lipid Metabolism - fatty acid

Chromosomal Location of Human Ortholog: 4q22-q26

Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; mitochondrial inner membrane; cytoplasm

Molecular Function: 3-hydroxyacyl-CoA dehydrogenase activity

Biological Process: response to drug; fatty acid beta-oxidation; response to activity; cellular lipid metabolic process; negative regulation of insulin secretion; response to insulin stimulus

Disease: 3-hydroxyacyl-coa Dehydrogenase Deficiency; Hyperinsulinemic Hypoglycemia, Familial, 4
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 Research Articles on HADH    1. Next-generation sequencing reveals deep intronic cryptic ABCC8 and HADH splicing founder mutations causing hyperinsulinism by pseudoexon activation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HADH elisa kitPathways associated with HADH elisa kit
 Reference Product  PubMed Publications
 SLC2A4 elisa kit  >1 publications with HADH and SLC2A4
 Products by Pathway  Pathway Diagram
 Beta Oxidation Of Butanoyl-CoA To Acetyl-CoA Pathway antibodies  Beta Oxidation Of Butanoyl-CoA To Acetyl-CoA Pathway Diagram
 Beta Oxidation Of Decanoyl-CoA To Octanoyl-CoA-CoA Pathway antibodies  Beta Oxidation Of Decanoyl-CoA To Octanoyl-CoA-CoA Pathway Diagram
 Beta Oxidation Of Hexanoyl-CoA To Butanoyl-CoA Pathway antibodies  Beta Oxidation Of Hexanoyl-CoA To Butanoyl-CoA Pathway Diagram
 Beta Oxidation Of Lauroyl-CoA To Decanoyl-CoA-CoA Pathway antibodies  Beta Oxidation Of Lauroyl-CoA To Decanoyl-CoA-CoA Pathway Diagram
 Beta Oxidation Of Octanoyl-CoA To Hexanoyl-CoA Pathway antibodies  Beta Oxidation Of Octanoyl-CoA To Hexanoyl-CoA Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies  FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
 Fatty Acid Beta Oxidation Pathway antibodies  Fatty Acid Beta Oxidation Pathway Diagram
 Fatty Acid Biosynthesis Pathway antibodies  Fatty Acid Biosynthesis Pathway Diagram
Diseases associated with HADH elisa kitOrgans/Tissues associated with HADH elisa kit
 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >5 publications with HADH and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >5 publications with HADH and Cardiovascular Diseases
 Heart Diseases Antibodies  >4 publications with HADH and Heart Diseases
 Brain Diseases Antibodies  >3 publications with HADH and Brain Diseases
 Seizures Antibodies  >2 publications with HADH and Seizures
 Fatigue Antibodies  >2 publications with HADH and Fatigue
 Fatty Liver Antibodies  >2 publications with HADH and Fatty Liver
 Mental Disorders Antibodies  >2 publications with HADH and Mental Disorders
 Myocardial Ischemia Antibodies  >2 publications with HADH and Myocardial Ischemia
 Nervous System Diseases Antibodies  >2 publications with HADH and Nervous System Diseases
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >54 publications with HADH and Muscle
 Blood Antibodies  >18 publications with HADH and Blood
 Liver Antibodies  >9 publications with HADH and Liver
 Brain Antibodies  >9 publications with HADH and Brain
 Adipose Tissue Antibodies  >8 publications with HADH and Adipose Tissue
 Heart Antibodies  >8 publications with HADH and Heart
 Pancreas Antibodies  >6 publications with HADH and Pancreas
 Connective Tissue Antibodies  >6 publications with HADH and Connective Tissue
 Lung Antibodies  >4 publications with HADH and Lung
 Kidney Antibodies  >3 publications with HADH and Kidney
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