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HIF-1alpha elisa kit :: Human hypoxia-inducible factor 1alpha (HIF-1alpha) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS703254
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

hypoxia-inducible factor 1alpha (HIF-1alpha), ELISA Kit

★Popular Item★
 Also Known As   

Human hypoxia-inducible factor 1alpha (HIF-1alpha) ELISA Kit

 Product Synonym Names    hypoxia-inducible factor 1alpha (HIF-1alpha); hypoxia-inducible factor 1a (HIF-1a) ; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8; bHLHe78; ARNT interacting protein|hypoxia-inducible factor 1 alpha isoform I.3|hypoxia-inducible factor 1; alpha subunit|hypoxi
 Product Gene Name   

HIF-1alpha elisa kit

[Similar Products]
 Product Synonym Gene Name    HIF-1a [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS703254 Testing Data    Testing Data PDF
 Chromosome Location    Chromosome: 14; NC_000014.8 (62162119..62214977). Location: 14q23.2
 OMIM    603348
 3D Structure    ModBase 3D Structure for Q16665
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human HIF-1alpha. No significant cross-reactivity or interference between human HIF-1alpha and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Quantitative Sandwich
 Detection Range    62.5 pg/ml - 4000 pg/ml
 Sensitivity    Typically less than 15.6 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HIF-1alpha elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HIF-1alpha purchase    MBS703254 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the hypoxia-inducible factor 1alpha (HIF-1alpha) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5 pg/ml - 4000 pg/ml in biological research samples containing HIF-1alpha, with an estimated sensitivity of Typically less than 15.6 pg/ml. The ELISA analytical biochemical technique of the MBS703254 kit is based on HIF-1alpha antibody-HIF-1alpha antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HIF-1alpha antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HIF-1alpha. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). A transcription factor turns on genes. Under low O2 conditions, hypoxia inducible factor 1 alpha translocates to the nucleus to generate functional HIF 1. To study function and expression, we recommend using our HIF 1 alpha ELISA kit. This species human ELISA kit, is in stock and comes complete with prebound mouse antibody plate, loading human positive control, secondary antibodies, standard curve protein, detection reagents and can be used as a single assay or in antibody arrays. Find our blog post and read full blog articles on this and other cell or molecular biology based custom services. Our catalog is full of ELISA kits of all sizes and prices to fit your needs. All our assays can also be custom tailored for your exact size requirements with strips instead of plates.
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Related Product Information for HIF-1alpha elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HIF-1alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HIF-1alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIF-1alpha is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIF-1alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of HIF-1alpha elisa kit    HIF-1alpha elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS703254. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HIF-1alpha. It may not necessarily be applicable to this product.
 NCBI GI #    4504385
 NCBI GeneID    3091
 NCBI Accession #    NP_001521.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001530.3 [Other Products]
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 UniProt Primary Accession #    Q16665 [Other Products]
 UniProt Secondary Accession #    Q53XP6; Q96PT9; Q9UPB1 [Other Products]
 UniProt Related Accession #    Q16665 [Other Products]
 Molecular Weight    92,670 Da
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 NCBI Official Full Name    hypoxia-inducible factor 1-alpha isoform 1
 NCBI Official Synonym Full Names    hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
 NCBI Official Symbol    HIF1A [Similar Products]
 NCBI Official Synonym Symbols   
HIF1; MOP1; PASD8; bHLHe78; HIF-1alpha; HIF1-ALPHA
[Similar Products]
 NCBI Protein Information    hypoxia-inducible factor 1-alpha; HIF-1-alpha; OTTHUMP00000179061; OTTHUMP00000179062; OTTHUMP00000179063; member of PAS protein 1; ARNT interacting protein; ARNT-interacting protein; member of PAS superfamily 1; PAS domain-containing protein 8; basic-helix-loop-helix-PAS protein MOP1; class E basic helix-loop-helix protein 78; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
 UniProt Protein Name    Hypoxia-inducible factor 1-alpha
 UniProt Synonym Protein Names   
ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS domain-containing protein 8
 UniProt Gene Name    HIF1A [Similar Products]
 UniProt Synonym Gene Names    BHLHE78; MOP1; PASD8 [Similar Products]
 UniProt Entry Name    HIF1A_HUMAN
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 NCBI Summary for HIF-1alpha    This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]
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 UniProt Comments for HIF-1alpha    Function: Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Ref.14 Ref.19 Ref.21 Ref.28 Ref.32 Ref.33 Ref.34

Subunit structure: Interacts with the HIF1A beta/ARNT subunit; heterodimerization is required for DNA binding. Interacts with COPS5; the interaction increases the transcriptional activity of HIF1A through increased stability

By similarity. Interacts with EP300 (via TAZ-type 1 domains); the interaction is stimulated in response to hypoxia and inhibited by CITED2. Interacts with CREBBP (via TAZ-type 1 domains). Interacts with NCOA1, NCOA2, APEX and HSP90. Interacts (hydroxylated within the ODD domain) with VHLL (via beta domain); the interaction, leads to polyubiquitination and subsequent HIF1A proteasomal degradation. During hypoxia, sumoylated HIF1A also binds VHL; the interaction promotes the ubiquitination of HIF1A. Interacts with SENP1; the interaction desumoylates HIF1A resulting in stabilization and activation of transcription. Interacts (Via the ODD domain) with ARD1A; the interaction appears not to acetylate HIF1A nor have any affect on protein stability, during hypoxia. Interacts with RWDD3; the interaction enhances HIF1A sumoylation. Interacts with TSGA10

By similarity. Interacts with RORA (via the DNA binding domain); the interaction enhances HIF1A transcription under hypoxia through increasing protein stability. Interaction with PSMA7 inhibits the transactivation activity of HIF1A under both normoxic and hypoxia-mimicking conditions. Interacts with USP20. Interacts with GNB2L1/RACK1; promotes HIF1A ubiquitination and proteasome-mediated degradation. Ref.9 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.19 Ref.20 Ref.22 Ref.27 Ref.29 Ref.30 Ref.31 Ref.32 Ref.33 Ref.35 Ref.36 Ref.37 Ref.38

Subcellular location: Cytoplasm. Nucleus. Note: Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia. Ref.11

Tissue specificity: Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.

Induction: Under reduced oxygen tension. Induced also by various receptor-mediated factors such as growth factors, cytokines, and circulatory factors such as PDGF, EGF, FGF2, IGF2, TGFB1, HGF, TNF, IL1B/interleukin-1 beta, angiotensin-2 and thrombin. However, this induction is less intense than that stimulated by hypoxia.

Domain: Contains two independent C-terminal transactivation domains, NTAD and CTAD, which function synergistically. Their transcriptional activity is repressed by an intervening inhibitory domain (ID). Ref.10 Ref.12 Ref.13

Post-translational modification: In normoxia, is hydroxylated on Pro-402 and Pro-564 in the oxygen-dependent degradation domain (ODD) by EGLN1/PHD1 and EGLN2/PHD2. EGLN3/PHD3 has also been shown to hydroxylate Pro-564. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Deubiquitinated by USP20. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.In normoxia, is hydroxylated on Asn-803 by HIF1AN, thus abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. This hydroxylation is inhibited by the Cu/Zn-chelator, Clioquinol.S-nitrosylation of Cys-800 may be responsible for increased recruitment of p300 coactivator necessary for transcriptional activity of HIF-1 complex.Requires phosphorylation for DNA-binding.Sumoylated; by SUMO1 under hypoxia. Sumoylation is enhanced through interaction with RWDD3. Desumoylation by SENP1 leads to increased HIF1A stability and transriptional activity

By similarity. Ref.28 Ref.34 Ref.35Ubiquitinated; in normoxia, following hydroxylation and interaction with VHL. Lys-532 appears to be the principal site of ubiquitination. Clioquinol, the Cu/Zn-chelator, inhibits ubiquitination through preventing hydroxylation at Asn-803. Ref.12 Ref.18 Ref.19 Ref.21 Ref.30 Ref.33 Ref.37 Ref.39The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.

Sequence similarities: Contains 1 basic helix-loop-helix (bHLH) domain.Contains 1 PAC (PAS-associated C-terminal) domain.Contains 2 PAS (PER-ARNT-SIM) domains.
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 Research Articles on HIF-1alpha    1. Hypoxia inducible factor-1alpha has a role in human emphysema lung tissue
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HIF-1alpha elisa kitPathways associated with HIF-1alpha elisa kit
 Reference Product  PubMed Publications
 VHL elisa kit  >436 publications with HIF-1alpha and VHL
 VEGFA elisa kit  >331 publications with HIF-1alpha and VEGFA
 ARNT elisa kit  >250 publications with HIF-1alpha and ARNT
 EPO elisa kit  >216 publications with HIF-1alpha and EPO
 EGLN1 elisa kit  >97 publications with HIF-1alpha and EGLN1
 JUN elisa kit  >83 publications with HIF-1alpha and JUN
 MAPK3 elisa kit  >72 publications with HIF-1alpha and MAPK3
 STAT3 elisa kit  >65 publications with HIF-1alpha and STAT3
 EGLN3 elisa kit  >53 publications with HIF-1alpha and EGLN3
 HIF1AN elisa kit  >50 publications with HIF-1alpha and HIF1AN
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Angiogenesis Pathway antibodies  Angiogenesis Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 Hypoxic And Oxygen Homeostasis Regulation Of HIF-1-alpha Pathway antibodies  Hypoxic And Oxygen Homeostasis Regulation Of HIF-1-alpha Pathway Diagram
 Notch-mediated HES/HEY Network Pathway antibodies  Notch-mediated HES/HEY Network Pathway Diagram
 PDGFR-beta Signaling Pathway antibodies  PDGFR-beta Signaling Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 Renal Cell Carcinoma Pathway antibodies  Renal Cell Carcinoma Pathway Diagram
 Renal Cell Carcinoma Pathway antibodies  Renal Cell Carcinoma Pathway Diagram
 Signaling Events Mediated By VEGFR1 And VEGFR2 Pathway antibodies  Signaling Events Mediated By VEGFR1 And VEGFR2 Pathway Diagram
Diseases associated with HIF-1alpha elisa kitOrgans/Tissues associated with HIF-1alpha elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2151 publications with HIF-1alpha and Neoplasms
 Neovascularization, Pathologic Antibodies  >632 publications with HIF-1alpha and Neovascularization, Pathologic
 Cardiovascular Diseases Antibodies  >566 publications with HIF-1alpha and Cardiovascular Diseases
 Adenocarcinoma Antibodies  >467 publications with HIF-1alpha and Adenocarcinoma
 Inflammation Antibodies  >278 publications with HIF-1alpha and Inflammation
 Breast Neoplasms Antibodies  >269 publications with HIF-1alpha and Breast Neoplasms
 Kidney Diseases Antibodies  >261 publications with HIF-1alpha and Kidney Diseases
 Lung Diseases Antibodies  >233 publications with HIF-1alpha and Lung Diseases
 Liver Diseases Antibodies  >231 publications with HIF-1alpha and Liver Diseases
 Neoplasm Metastasis Antibodies  >205 publications with HIF-1alpha and Neoplasm Metastasis
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >2234 publications with HIF-1alpha and Vascular
 Blood Antibodies  >1242 publications with HIF-1alpha and Blood
 Muscle Antibodies  >470 publications with HIF-1alpha and Muscle
 Brain Antibodies  >459 publications with HIF-1alpha and Brain
 Kidney Antibodies  >434 publications with HIF-1alpha and Kidney
 Liver Antibodies  >372 publications with HIF-1alpha and Liver
 Lung Antibodies  >363 publications with HIF-1alpha and Lung
 Heart Antibodies  >347 publications with HIF-1alpha and Heart
 Bone Antibodies  >233 publications with HIF-1alpha and Bone
 Embryonic Tissue Antibodies  >203 publications with HIF-1alpha and Embryonic Tissue
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