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IGFBP3 elisa kit :: Human insulin-like growth factor binding protein 3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700339
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $420 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,495 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,715 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

insulin-like growth factor binding protein 3 (IGFBP3), ELISA Kit

 Also Known As   

Human insulin-like growth factors binding protein 3, IGFBP-3 ELISA Kit

 Product Synonym Names    Human insulin-like growth factors binding protein 3; IGFBP-3 ELISA Kit; tcag7.703; BP-53; IBP3; IGF-binding protein 3; acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; growth hormone-dependent binding protein; insulin-like growth factor binding protein 3
 Product Gene Name   

IGFBP3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS700339 Testing Data    Testing Data PDF
 OMIM    146732
 3D Structure    ModBase 3D Structure for P17936
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human IGFBP3. No significant cross-reactivity or interference between Human IGFBP3 and analogues was observed.
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 Samples    Serum, plasma and tissue homogenates
 Detection Range    0.78 ng/ml-50 ng/ml
 Sensitivity    0.195 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IGFBP3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IGFBP3 purchase    MBS700339 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the insulin-like growth factor binding protein 3 (IGFBP3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.78 ng/ml-50 ng/ml in biological research samples containing IGFBP3, with an estimated sensitivity of 0.195 ng/ml. The ELISA analytical biochemical technique of the MBS700339 kit is based on IGFBP3 antibody-IGFBP3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IGFBP3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IGFBP3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IGFBP3 elisa kit

   Introduction: Insulin-like growth factor binding protein 3, also known as IGFBP3, is a protein which in humans is encoded by the IGFBP3 gene. This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. IGFBP3 protein levels decrease during the progression of prostate cancer from benign prostatic hyperplasia (BPH) to its metastatic form. Although, production of the protein does not cease completely. IGFBP3 is still made (at a lower level) by prostate cancer cells and secreted into the surrounding environment. However, instead of the full length, functional protein, IGFBP3 is found to be cleaved.This prevents IGFBP3 from binding and sequestering IGFs and the growth factors are free to bind the IGF-1R and promote cell survival.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IGFBP-3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IGFBP-3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IGFBP-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IGFBP-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of IGFBP3 elisa kit    IGFBP3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS700339. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IGFBP3. It may not necessarily be applicable to this product.
 NCBI GI #    62243068
 NCBI GeneID    3486
 NCBI Accession #    NP_000589.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000598.4 [Other Products]
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 UniProt Primary Accession #    P17936 [Other Products]
 UniProt Secondary Accession #    Q2V509; Q6P1M6; Q9UCL4; A4D2F5; D3DVM0 [Other Products]
 UniProt Related Accession #    P17936 [Other Products]
 Molecular Weight    31,674 Da
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 NCBI Official Full Name    insulin-like growth factor-binding protein 3 isoform b
 NCBI Official Synonym Full Names    insulin-like growth factor binding protein 3
 NCBI Official Symbol    IGFBP3 [Similar Products]
 NCBI Official Synonym Symbols   
IBP3; BP-53
[Similar Products]
 NCBI Protein Information    insulin-like growth factor-binding protein 3; IBP-3; IGFBP-3; binding protein 29; binding protein 53; IGF-binding protein 3; growth hormone-dependent binding protein; acid stable subunit of the 140 K IGF complex
 UniProt Protein Name    Insulin-like growth factor-binding protein 3
 Protein Family    Insulin-like growth factor-binding protein
 UniProt Gene Name    IGFBP3 [Similar Products]
 UniProt Synonym Gene Names    IBP3; IBP-3; IGF-binding protein 3; IGFBP-3 [Similar Products]
 UniProt Entry Name    IBP3_HUMAN
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 NCBI Summary for IGFBP3    This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
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 UniProt Comments for IGFBP3    Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.1 PublicationManual assertion based on experiment in:Ref.17
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 Research Articles on IGFBP3    1. High hepatic IGFBP-3 expression is associated with chronic hepatitis C.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IGFBP3 elisa kitPathways associated with IGFBP3 elisa kit
 Reference Product  PubMed Publications
 IGF1 elisa kit  >207 publications with IGFBP3 and IGF1
 IGF2 elisa kit  >73 publications with IGFBP3 and IGF2
 IGFALS elisa kit  >19 publications with IGFBP3 and IGFALS
 TP53 elisa kit  >11 publications with IGFBP3 and TP53
 MMP2 elisa kit  >5 publications with IGFBP3 and MMP2
 ADAM12 elisa kit  >2 publications with IGFBP3 and ADAM12
 FN1 elisa kit  >1 publications with IGFBP3 and FN1
 Products by Pathway  Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Myometrial Relaxation And Contraction Pathways antibodies  Myometrial Relaxation And Contraction Pathways Diagram
 Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway antibodies  Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 P53 Signaling Pathway antibodies  P53 Signaling Pathway Diagram
 P53 Signaling Pathway antibodies  P53 Signaling Pathway Diagram
Diseases associated with IGFBP3 elisa kitOrgans/Tissues associated with IGFBP3 elisa kit
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >341 publications with IGFBP3 and Breast Neoplasms
 Prostatic Neoplasms Antibodies  >256 publications with IGFBP3 and Prostatic Neoplasms
 Cardiovascular Diseases Antibodies  >220 publications with IGFBP3 and Cardiovascular Diseases
 Adenocarcinoma Antibodies  >172 publications with IGFBP3 and Adenocarcinoma
 Weight Gain Antibodies  >145 publications with IGFBP3 and Weight Gain
 Colorectal Neoplasms Antibodies  >144 publications with IGFBP3 and Colorectal Neoplasms
 Liver Diseases Antibodies  >142 publications with IGFBP3 and Liver Diseases
 Lung Diseases Antibodies  >136 publications with IGFBP3 and Lung Diseases
 Inflammation Antibodies  >127 publications with IGFBP3 and Inflammation
 Kidney Diseases Antibodies  >123 publications with IGFBP3 and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >371 publications with IGFBP3 and Liver
 Muscle Antibodies  >260 publications with IGFBP3 and Muscle
 Connective Tissue Antibodies  >199 publications with IGFBP3 and Connective Tissue
 Kidney Antibodies  >192 publications with IGFBP3 and Kidney
 Brain Antibodies  >173 publications with IGFBP3 and Brain
 Lung Antibodies  >153 publications with IGFBP3 and Lung
 Ovary Antibodies  >150 publications with IGFBP3 and Ovary
 Heart Antibodies  >112 publications with IGFBP3 and Heart
 Placenta Antibodies  >69 publications with IGFBP3 and Placenta
 Embryonic Tissue Antibodies  >69 publications with IGFBP3 and Embryonic Tissue
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