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ITIH4 elisa kit :: Human inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS705980
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein) (ITIH4), ELISA Kit

★Popular Item★
 Also Known As   

Human Inter-alpha-trypsin inhibitor heavy chain H4, ITIH4/IHRP/ITIHL1/PK120/PRO1851 ELISA Kit

 Product Synonym Names    Human Inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4/IHRP/ITIHL1/PK120/PRO1851) ELISA kit; DKFZp686G21125; H4P; IHRP; ITIHL1; PK120; inter-alpha (globulin) inhibitor H4; inter-alpha-trypsin inhibitor; heavy chain-like; 1; inter-alpha-trypsin inhibitor; heavy chain-related protein; plasma kal; inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein)
 Product Gene Name   

ITIH4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS705980 Testing Data    Testing Data PDF
 OMIM    600564
 3D Structure    ModBase 3D Structure for Q14624
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human ITIH4 . No significant cross-reactivity or interference between human ITIH4 and analogues was observed.
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 Samples    Serum, plasma and tissue homogenates
 Assay Type    Sandwich
 Detection Range    0.625 ng/ml -40 ng/ml.
 Sensitivity    The minimum detectable dose of human ITIH4 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ITIH4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ITIH4 purchase    MBS705980 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein) (ITIH4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625 ng/ml -40 ng/ml in biological research samples containing ITIH4, with an estimated sensitivity of The minimum detectable dose of human ITIH4 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS705980 kit is based on ITIH4 antibody-ITIH4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ITIH4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ITIH4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ITIH4 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ITIH4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ITIH4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ITIH4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ITIH4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ITIH4 elisa kit    ITIH4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS705980. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ITIH4. It may not necessarily be applicable to this product.
 NCBI GI #    262050538
 NCBI GeneID    3700
 NCBI Accession #    NP_001159921.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001166449.1 [Other Products]
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 UniProt Primary Accession #    Q14624 [Other Products]
 UniProt Secondary Accession #    Q15135; Q9P190; Q9UQ54; B7Z545; E9PGN5 [Other Products]
 UniProt Related Accession #    Q14624 [Other Products]
 Molecular Weight    103,357 Da
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 NCBI Official Full Name    inter-alpha-trypsin inhibitor heavy chain H4 isoform 2
 NCBI Official Synonym Full Names    inter-alpha-trypsin inhibitor heavy chain family, member 4
 NCBI Official Symbol    ITIH4 [Similar Products]
 NCBI Official Synonym Symbols   
H4P; IHRP; GP120; PK120; ITIHL1; PK-120; ITI-HC4
[Similar Products]
 NCBI Protein Information    inter-alpha-trypsin inhibitor heavy chain H4; ITI heavy chain H4; inter-alpha-inhibitor heavy chain 4; plasma kallikrein-sensitive glycoprotein 120; inter-alpha-trypsin inhibitor, heavy chain-like, 1; inter-alpha-trypsin inhibitor family heavy chain-related protein; inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein)
 UniProt Protein Name    Inter-alpha-trypsin inhibitor heavy chain H4
 UniProt Synonym Protein Names   
Inter-alpha-trypsin inhibitor family heavy chain-related protein; IHRP; Plasma kallikrein sensitive glycoprotein 120; Gp120; PK-120
 Protein Family    Inter alpha-trypsin inhibitor
 UniProt Gene Name    ITIH4 [Similar Products]
 UniProt Synonym Gene Names    IHRP; ITIHL1; PK120; PRO1851; ITI heavy chain H4; ITI-HC4; Inter-alpha-inhibitor heavy chain 4; IHRP; Gp120; PK-120 [Similar Products]
 UniProt Entry Name    ITIH4_HUMAN
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 NCBI Summary for ITIH4    The protein encoded by this gene is secreted into the blood, where it is cleaved by plasma kallikrein into two smaller forms. Expression of this gene has been detected only in liver, and it seems to be upregulated during surgical trauma. This gene is part of a cluster of similar genes on chromosome 3. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
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 UniProt Comments for ITIH4    Function: Type II acute-phase protein (APP) involved in inflammatory responses to trauma. May also play a role in liver development or regeneration.1 PublicationManual assertion based on experiment in:Ref.14
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 Research Articles on ITIH4    1. Expression of the 85 kDa ITIH4 was substantial in amyotrophic lateral sclerosis compared with controls or patients with muscular dystrophy, Alzheimer diseases, or Parkinson diseases
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ITIH4 elisa kitDiseases associated with ITIH4 elisa kit
 Reference Product  PubMed Publications
 AMBP elisa kit  >1 publications with ITIH4 and AMBP
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >11 publications with ITIH4 and Inflammation
 Liver Diseases Antibodies  >6 publications with ITIH4 and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >4 publications with ITIH4 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >3 publications with ITIH4 and Liver Neoplasms
 Disease Models, Animal Antibodies  >3 publications with ITIH4 and Disease Models, Animal
 Liver Cirrhosis Antibodies  >3 publications with ITIH4 and Liver Cirrhosis
 Cardiovascular Diseases Antibodies  >3 publications with ITIH4 and Cardiovascular Diseases
 Lung Diseases Antibodies  >3 publications with ITIH4 and Lung Diseases
 Fibrosis Antibodies  >3 publications with ITIH4 and Fibrosis
 Brain Diseases Antibodies  >3 publications with ITIH4 and Brain Diseases
Organs/Tissues associated with ITIH4 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >42 publications with ITIH4 and Blood
 Liver Antibodies  >15 publications with ITIH4 and Liver
 Lung Antibodies  >3 publications with ITIH4 and Lung
 Connective Tissue Antibodies  >2 publications with ITIH4 and Connective Tissue
 Pancreas Antibodies  >2 publications with ITIH4 and Pancreas
 Brain Antibodies  >2 publications with ITIH4 and Brain
 Prostate Antibodies  >1 publications with ITIH4 and Prostate
 Uterus Antibodies  >1 publications with ITIH4 and Uterus
 Ovary Antibodies  >1 publications with ITIH4 and Ovary
 Placenta Antibodies  >1 publications with ITIH4 and Placenta
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