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IL8 elisa kit :: Human interleukin 8 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS703104 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $320 +1 FREE 8GB USB
96-Strip-Wells  /  $445 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,880 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,540 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

interleukin 8 (IL8), ELISA Kit

★Popular Item★
 Also Known As   

Human Interleukin 8, IL-8 ELISA Kit

 Product Synonym Names    Human Interleukin 8; IL-8 ELISA KIT; CXCL8; GCP-1; GCP1; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1; T cell chemotactic factor; beta-thromboglobulin-like protein; chemokine (C-X-C motif) ligand 8; emoctakin; granulocyte chemotactic; Human Interleukin 8; IL-8
 Product Gene Name   

IL8 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS703104 Testing Data    Testing Data PDF
 OMIM    146930
 3D Structure    ModBase 3D Structure for P10145
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human IL-8. No significant cross-reactivity or interference between human IL-8 and analogues was observed.
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 Samples    Serum, cell culture supernates, saliva, urine, saliva
 Assay Type    Quantitative Sandwich
 Detection Range    31.25 pg/ml -2000 pg/ml
 Sensitivity    Typically less than 7.110 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL8 purchase    MBS703104 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the interleukin 8 (IL8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml -2000 pg/ml in biological research samples containing IL8, with an estimated sensitivity of Typically less than 7.110 pg/ml. The ELISA analytical biochemical technique of the MBS703104 kit is based on IL8 antibody-IL8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, cell culture supernates, saliva, urine, saliva. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL8 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of IL8 elisa kit    IL8 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS703104. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL8. It may not necessarily be applicable to this product.
 NCBI GI #    10834978
 NCBI GeneID    3576
 NCBI Accession #    NP_000575.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000584.3 [Other Products]
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 UniProt Primary Accession #    P10145 [Other Products]
 UniProt Secondary Accession #    Q6FGF6; Q6LAE6; Q96RG6; Q9C077; Q9UCE1; Q9UCR8; Q9UCR9; Q9UCS0; B2R4L8 [Other Products]
 UniProt Related Accession #    P10145 [Other Products]
 Molecular Weight    11,098 Da
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 NCBI Official Full Name    interleukin-8
 NCBI Official Synonym Full Names    chemokine (C-X-C motif) ligand 8
 NCBI Official Symbol    CXCL8 [Similar Products]
 NCBI Official Synonym Symbols   
IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
[Similar Products]
 NCBI Protein Information    interleukin-8; emoctakin; interleukin 8; T-cell chemotactic factor; neutrophil-activating peptide 1; beta-thromboglobulin-like protein; granulocyte chemotactic protein 1; tumor necrosis factor-induced gene 1; alveolar macrophage chemotactic factor I; monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; small inducible cytokine subfamily B, member 8; lymphocyte derived neutrophil activating peptide; lung giant cell carcinoma-derived chemotactic protein; beta endothelial cell-derived neutrophil activating peptide
 UniProt Protein Name    Interleukin-8
 UniProt Synonym Protein Names   
C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1; Monocyte-derived neutrophil chemotactic factor; MDNCF; Monocyte-derived neutrophil-activating peptide; MONAP; Neutrophil-activating protein 1; NAP-1; Protein 3-10C; T-cell chemotactic factorCleaved into the following 7 chains:MDNCF-aAlternative name(s):GCP/IL-8 protein IV; IL8/NAP1 form I
 UniProt Gene Name    CXCL8 [Similar Products]
 UniProt Synonym Gene Names    IL8; IL-8; GCP-1; MDNCF; MONAP; NAP-1; LYNAP; NAF [Similar Products]
 UniProt Entry Name    IL8_HUMAN
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 NCBI Summary for IL8    The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq, Jul 2008]
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 UniProt Comments for IL8    Function: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.3 PublicationsManual assertion based on experiment in:Ref.15
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 Research Articles on IL8    1. IL-8 secretion from SW982 synovial sarcoma cells suppressed by pyrin independently of inflammasome is affected by pyrin mutations.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with IL8 elisa kitDiseases associated with IL8 elisa kit
 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 ATF4 Activates Genes Pathway antibodies  ATF4 Activates Genes Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >585 publications with IL8 and Inflammation
 Necrosis Antibodies  >312 publications with IL8 and Necrosis
 Pulmonary Disease, Chronic Obstructive Antibodies  >78 publications with IL8 and Pulmonary Disease, Chronic Obstructive
 Adenocarcinoma Antibodies  >30 publications with IL8 and Adenocarcinoma
 Breast Neoplasms Antibodies  >23 publications with IL8 and Breast Neoplasms
 Kidney Diseases Antibodies  >20 publications with IL8 and Kidney Diseases
 Colorectal Neoplasms Antibodies  >15 publications with IL8 and Colorectal Neoplasms
 Prostatic Neoplasms Antibodies  >14 publications with IL8 and Prostatic Neoplasms
 Pulmonary Fibrosis Antibodies  >14 publications with IL8 and Pulmonary Fibrosis
 Carcinoma, Non-Small-Cell Lung Antibodies  >13 publications with IL8 and Carcinoma, Non-Small-Cell Lung
Organs/Tissues associated with IL8 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >544 publications with IL8 and Blood
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