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KIRREL elisa kit :: Human kin of IRRE like (Drosophila) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS917536
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

kin of IRRE like (Drosophila) (KIRREL), ELISA Kit

 Also Known As   

Human Kin of IRRE-like protein 1, KIRREL ELISA Kit

 Product Synonym Names    Human Kin of IRRE-like protein 1 (KIRREL) ELISA kit; FLJ10845; MGC129542; MGC129543; NEPH1; kin of IRRE like; nephrin related; kin of IRRE like (Drosophila)
 Product Gene Name   

KIRREL elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS917536 Testing Data    Testing Data PDF
 OMIM    607428
 3D Structure    ModBase 3D Structure for Q96J84
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human KIRREL. No significant cross-reactivity or interference between human KIRREL and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    47 pg/ml -3000 pg/ml.
 Sensitivity    The minimum detectable dose of human KIRREL is typically less than 11.75 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of KIRREL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for KIRREL purchase    MBS917536 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the kin of IRRE like (Drosophila) (KIRREL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 47 pg/ml -3000 pg/ml in biological research samples containing KIRREL, with an estimated sensitivity of The minimum detectable dose of human KIRREL is typically less than 11.75 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS917536 kit is based on KIRREL antibody-KIRREL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KIRREL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KIRREL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for KIRREL elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KIRREL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KIRREL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KIRREL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIRREL bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of KIRREL elisa kit    KIRREL elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS917536. Click to request current manual
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NCBI/Uniprot data below describe general gene information for KIRREL. It may not necessarily be applicable to this product.
 NCBI GI #    556503362
 NCBI GeneID    55243
 NCBI Accession #    NP_001273278.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001286349.1 [Other Products]
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 UniProt Primary Accession #    Q96J84 [Other Products]
 UniProt Secondary Accession #    Q5W0F8; Q5XKC6; Q7Z696; Q7Z7N8; Q8TB15; Q9H9N1; Q9NVA5 [Other Products]
 UniProt Related Accession #    Q96J84 [Other Products]
 Molecular Weight    83,536 Da
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 NCBI Official Full Name    kin of IRRE-like protein 1 isoform 2
 NCBI Official Synonym Full Names    kin of IRRE like (Drosophila)
 NCBI Official Symbol    KIRREL [Similar Products]
 NCBI Official Synonym Symbols   
NEPH1
[Similar Products]
 NCBI Protein Information    kin of IRRE-like protein 1; nephrin related; nephrin-like protein 1; kin of irregular chiasm-like protein 1
 UniProt Protein Name    Kin of IRRE-like protein 1
 UniProt Synonym Protein Names   
Kin of irregular chiasm-like protein 1; Nephrin-like protein 1
 Protein Family    Kin of IRRE-like protein
 UniProt Gene Name    KIRREL [Similar Products]
 UniProt Synonym Gene Names    KIRREL1; NEPH1 [Similar Products]
 UniProt Entry Name    KIRR1_HUMAN
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 NCBI Summary for KIRREL    NEPH1 is a member of the nephrin-like protein family, which includes NEPH2 (MIM 607761) and NEPH3 (MIM 607762). The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2; MIM 604766), and the genes are expressed in kidney podocytes, cells involved in ensuring size- and charge-selective ultrafiltration (Sellin et al., 2003 [PubMed 12424224]).[supplied by OMIM, Mar 2008]
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 UniProt Comments for KIRREL    KIRREL: Plays a significant role in the normal development and function of the glomerular permeability. Signaling protein that needs the presence of TEC kinases to fully trans-activate the transcription factor AP-1. Belongs to the immunoglobulin superfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: perinuclear region of cytoplasm; cell projection membrane; integral to membrane; plasma membrane; intercellular junction; dendritic shaft; lipid raft

Molecular Function: protein binding; myosin binding

Biological Process: cell-cell adhesion; positive regulation of actin filament polymerization; negative regulation of protein amino acid phosphorylation; excretion
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 Research Articles on KIRREL    1. maintaining high levels of Neph1 at the membrane using a podocyte cell line overexpressing chimeric Neph1 increased the ability of podocytes to resist PAN-induced injury and PAN-induced albumin leakage
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with KIRREL elisa kitPathways associated with KIRREL elisa kit
 Reference Product  PubMed Publications
 NPHS2 elisa kit  >6 publications with KIRREL and NPHS2
 NPHS1 elisa kit  >4 publications with KIRREL and NPHS1
 TJP1 elisa kit  >1 publications with KIRREL and TJP1
 Products by Pathway  Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Nephrin Interactions Pathway antibodies  Nephrin Interactions Pathway Diagram
 Nephrin/Neph1 Signaling In The Kidney Podocyte Pathway antibodies  Nephrin/Neph1 Signaling In The Kidney Podocyte Pathway Diagram
Diseases associated with KIRREL elisa kitOrgans/Tissues associated with KIRREL elisa kit
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >7 publications with KIRREL and Kidney Diseases
 Nephrotic Syndrome Antibodies  >7 publications with KIRREL and Nephrotic Syndrome
 Proteinuria Antibodies  >7 publications with KIRREL and Proteinuria
 Nephrosis Antibodies  >3 publications with KIRREL and Nephrosis
 Disease Models, Animal Antibodies  >2 publications with KIRREL and Disease Models, Animal
 Hyperglycemia Antibodies  >1 publications with KIRREL and Hyperglycemia
 Hypertension Antibodies  >1 publications with KIRREL and Hypertension
 Edema Antibodies  >1 publications with KIRREL and Edema
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >18 publications with KIRREL and Kidney
 Muscle Antibodies  >3 publications with KIRREL and Muscle
 Brain Antibodies  >2 publications with KIRREL and Brain
 Embryonic Tissue Antibodies  >1 publications with KIRREL and Embryonic Tissue
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