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LDHB elisa kit :: Human lactate dehydrogenase B ELISA Kit

Scan QR to view Datasheet Catalog #    MBS928418
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

lactate dehydrogenase B (LDHB), ELISA Kit

★Popular Item★
 Also Known As   

Human L-lactate dehydrogenase B chain, LDHB ELISA Kit

 Product Synonym Names    Human L-lactate dehydrogenase B chain (LDHB) ELISA kit; LDH-H; TRG-5; L-lactate dehydrogenase B; OTTHUMP00000165229; lactate dehydrogenase B
 Product Gene Name   

LDHB elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS928418 Testing Data    Testing Data PDF
 OMIM    gene 614128
 3D Structure    ModBase 3D Structure for P07195
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human LDHB. No significant cross-reactivity or interference between human LDHB and analogues was observed.
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 Samples    Serum, plasma, urine, cerebrospinal fluid (CSF), tissue homogenates
 Assay Type    Sandwich
 Detection Range    23.5 mU/ml-1500 mU/ml.
 Sensitivity    The minimum detectable dose of human LDHB is typically less than 5.8 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of LDHB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for LDHB purchase    MBS928418 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the lactate dehydrogenase B (LDHB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 23.5 mU/ml-1500 mU/ml in biological research samples containing LDHB, with an estimated sensitivity of The minimum detectable dose of human LDHB is typically less than 5.8 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS928418 kit is based on LDHB antibody-LDHB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LDHB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LDHB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, urine, cerebrospinal fluid (CSF), tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for LDHB elisa kit

   Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LDHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LDHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LDHB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LDHB bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of LDHB elisa kit    LDHB elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS928418. Click to request current manual
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NCBI/Uniprot data below describe general gene information for LDHB. It may not necessarily be applicable to this product.
 NCBI GI #    291575128
 NCBI GeneID    3945
 NCBI Accession #    NP_001167568.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001174097.1 [Other Products]
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 UniProt Primary Accession #    P07195 [Other Products]
 UniProt Related Accession #    P07195 [Other Products]
 Molecular Weight    36,638 Da
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 NCBI Official Full Name    L-lactate dehydrogenase B chain
 NCBI Official Synonym Full Names    lactate dehydrogenase B
 NCBI Official Symbol    LDHB [Similar Products]
 NCBI Official Synonym Symbols   
LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
[Similar Products]
 NCBI Protein Information    L-lactate dehydrogenase B chain; LDH heart subunit; lactate dehydrogenase H chain; renal carcinoma antigen NY-REN-46; epididymis secretory protein Li 281
 UniProt Protein Name    L-lactate dehydrogenase B chain
 UniProt Synonym Protein Names   
LDH heart subunit; LDH-H; Renal carcinoma antigen NY-REN-46
 Protein Family    L-lactate dehydrogenase
 UniProt Gene Name    LDHB [Similar Products]
 UniProt Synonym Gene Names    LDH-B; LDH-H [Similar Products]
 UniProt Entry Name    LDHB_HUMAN
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 NCBI Summary for LDHB    This gene encodes an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on the X chromosome and on chromosome. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Mar 2010]
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 Research Articles on LDHB    1. This meta-analysis shows that high serum LDH level is obviously associated with lower overall survival rate in patients with osteosarcoma, and it is an effective biomarker of prognosis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with LDHB elisa kitDiseases associated with LDHB elisa kit
 Products by Pathway  Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Glycolysis And Gluconeogenesis Pathway antibodies  Glycolysis And Gluconeogenesis Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Propanoate Metabolism Pathway antibodies  Propanoate Metabolism Pathway Diagram
 Propanoate Metabolism Pathway antibodies  Propanoate Metabolism Pathway Diagram
 Pyruvate Metabolism Pathway antibodies  Pyruvate Metabolism Pathway Diagram
 Pyruvate Metabolism Pathway antibodies  Pyruvate Metabolism Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >20 publications with LDHB and Neoplasms
 Carcinoma Antibodies  >8 publications with LDHB and Carcinoma
 Neuroectodermal Tumors Antibodies  >5 publications with LDHB and Neuroectodermal Tumors
 Adenocarcinoma Antibodies  >4 publications with LDHB and Adenocarcinoma
 Nervous System Diseases Antibodies  >4 publications with LDHB and Nervous System Diseases
 Carcinoma, Small Cell Antibodies  >4 publications with LDHB and Carcinoma, Small Cell
 Neoplasm Metastasis Antibodies  >4 publications with LDHB and Neoplasm Metastasis
 Colorectal Neoplasms Antibodies  >3 publications with LDHB and Colorectal Neoplasms
 Congenital Abnormalities Antibodies  >3 publications with LDHB and Congenital Abnormalities
 Intestinal Diseases Antibodies  >3 publications with LDHB and Intestinal Diseases
Organs/Tissues associated with LDHB elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >27 publications with LDHB and Blood
 Testis Antibodies  >10 publications with LDHB and Testis
 Muscle Antibodies  >9 publications with LDHB and Muscle
 Brain Antibodies  >8 publications with LDHB and Brain
 Heart Antibodies  >7 publications with LDHB and Heart
 Liver Antibodies  >3 publications with LDHB and Liver
 Bone Antibodies  >3 publications with LDHB and Bone
 Lung Antibodies  >2 publications with LDHB and Lung
 Lymph Node Antibodies  >2 publications with LDHB and Lymph Node
 Thymus Antibodies  >2 publications with LDHB and Thymus
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