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LDHC elisa kit :: Human lactate dehydrogenase C ELISA Kit

Scan QR to view Datasheet Catalog #    MBS906075
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

lactate dehydrogenase C (LDHC), ELISA Kit

★Popular Item★
 Also Known As   

Human L-lactate dehydrogenase C chain, LDHC ELISA Kit

 Product Synonym Names    Human L-lactate dehydrogenase C chain (LDHC) ELISA kit; CT32; LDH3; LDHX; MGC111073; L-lactate dehydrogenase C; cancer/testis antigen 32; lactate dehydrogenase C
 Product Gene Name   

LDHC elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS906075 Testing Data    Testing Data PDF
 OMIM    150150
 3D Structure    ModBase 3D Structure for P07864
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human LDHC. No significant cross-reactivity or interference between human LDHC and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, urine
 Assay Type    Sandwich
 Detection Range    6.25 mU/ml-400 mU/ml.
 Sensitivity    The minimum detectable dose of human LDHC is typically less than 1.56 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of LDHC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for LDHC purchase    MBS906075 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the lactate dehydrogenase C (LDHC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6.25 mU/ml-400 mU/ml in biological research samples containing LDHC, with an estimated sensitivity of The minimum detectable dose of human LDHC is typically less than 1.56 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS906075 kit is based on LDHC antibody-LDHC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LDHC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LDHC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, urine. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for LDHC elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LDHC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LDHC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LDHC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LDHC bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of LDHC elisa kit    LDHC elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS906075. Click to request current manual
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NCBI/Uniprot data below describe general gene information for LDHC. It may not necessarily be applicable to this product.
 NCBI GI #    4504973
 NCBI GeneID    3948
 NCBI Accession #    NP_002292.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002301.4 [Other Products]
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 UniProt Primary Accession #    P07864 [Other Products]
 UniProt Secondary Accession #    Q6GSG8; Q7Z7J4; D3DQY4 [Other Products]
 UniProt Related Accession #    P07864 [Other Products]
 Molecular Weight    36,311 Da
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 NCBI Official Full Name    L-lactate dehydrogenase C chain
 NCBI Official Synonym Full Names    lactate dehydrogenase C
 NCBI Official Symbol    LDHC [Similar Products]
 NCBI Official Synonym Symbols   
CT32; LDH3; LDHX
[Similar Products]
 NCBI Protein Information    L-lactate dehydrogenase C chain; LDH-C; LDH-X; LDH testis subunit; cancer/testis antigen 32; lactate dehydrogenase C4; lactate dehydrogenase c variant 1; lactate dehydrogenase c variant 3; lactate dehydrogenase c variant 4
 UniProt Protein Name    L-lactate dehydrogenase C chain
 UniProt Synonym Protein Names   
Cancer/testis antigen 32; CT32; LDH testis subunit; LDH-X
 Protein Family    L-lactate dehydrogenase
 UniProt Gene Name    LDHC [Similar Products]
 UniProt Synonym Gene Names    LDH3; LDHX; LDH-C; CT32 [Similar Products]
 UniProt Entry Name    LDHC_HUMAN
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 NCBI Summary for LDHC    Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHC is testis-specific and belongs to the lactate dehydrogenase family. Two transcript variants have been detected which differ in the 5' untranslated region. [provided by RefSeq, Jul 2008]
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 UniProt Comments for LDHC    LDH-C: Possible role in sperm motility. Belongs to the LDH/MDH superfamily. LDH family.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 1.1.1.27; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - cysteine and methionine; Cancer Testis Antigen (CTA); Oxidoreductase

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: cytoplasm; nucleus

Molecular Function: L-lactate dehydrogenase activity

Biological Process: sperm motility; ATP biosynthetic process; cellular carbohydrate metabolic process; lactate oxidation; lactate biosynthetic process from pyruvate
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 Research Articles on LDHC    1. Results show that S-100B, MIA and LDH levels were significantly higher in patients with advanced melanoma than in disease-free patients or healthy controls.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with LDHC elisa kitDiseases associated with LDHC elisa kit
 Products by Pathway  Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Glycolysis And Gluconeogenesis Pathway antibodies  Glycolysis And Gluconeogenesis Pathway Diagram
 Propanoate Metabolism Pathway antibodies  Propanoate Metabolism Pathway Diagram
 Propanoate Metabolism Pathway antibodies  Propanoate Metabolism Pathway Diagram
 Pyruvate Metabolism Pathway antibodies  Pyruvate Metabolism Pathway Diagram
 Pyruvate Metabolism Pathway antibodies  Pyruvate Metabolism Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >6 publications with LDHC and Neoplasms
 Infertility, Male Antibodies  >5 publications with LDHC and Infertility, Male
 Lung Neoplasms Antibodies  >3 publications with LDHC and Lung Neoplasms
 Testicular Diseases Antibodies  >2 publications with LDHC and Testicular Diseases
 Lung Diseases Antibodies  >2 publications with LDHC and Lung Diseases
 Adenocarcinoma Antibodies  >1 publications with LDHC and Adenocarcinoma
 Melanoma Antibodies  >1 publications with LDHC and Melanoma
 Necrosis Antibodies  >1 publications with LDHC and Necrosis
 Spinal Cord Injuries Antibodies  >1 publications with LDHC and Spinal Cord Injuries
 Death Antibodies  >1 publications with LDHC and Death
Organs/Tissues associated with LDHC elisa kit
 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >26 publications with LDHC and Testis
 Lung Antibodies  >3 publications with LDHC and Lung
 Placenta Antibodies  >1 publications with LDHC and Placenta
 Brain Antibodies  >1 publications with LDHC and Brain
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