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LEPR elisa kit :: Human leptin receptor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS703701
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $625 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,905 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

leptin receptor (LEPR), ELISA Kit

★Popular Item★
 Also Known As   

Human Leptin receptor, LR/Ob-R ELISA Kit

 Product Synonym Names    Human Leptin receptor; LR/Ob-R ELISA KIT; CD295; OBR; OB receptor; OTTHUMP00000010694; leptin receptor
 Product Gene Name   

LEPR elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS703701 Testing Data    Testing Data PDF
 OMIM    gene 614963
 3D Structure    ModBase 3D Structure for P48357
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human LEPR. No significant cross-reactivity or interference between Human LEPR and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Detection Range    3.12 pg/ml-200 pg/ml
 Sensitivity    0.78 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of LEPR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for LEPR purchase    MBS703701 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the leptin receptor (LEPR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.12 pg/ml-200 pg/ml in biological research samples containing LEPR, with an estimated sensitivity of 0.78 pg/ml. The ELISA analytical biochemical technique of the MBS703701 kit is based on LEPR antibody-LEPR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LEPR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LEPR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for LEPR elisa kit

   For samples: This immunoassay kit allows for the in vitro quantitative determination of human LR/Ob-R concentrations in serum, plasma, cell Culture supernates, tissue homogenates.

PRINCIPLE OF THE ASSAY The microtiter plate provided in this kit has been pre-coated with an antibody specific to LR/Ob-R. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for LR/Ob-R and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain LR/Ob-R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of LR/Ob-R in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of LEPR elisa kit    LEPR elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS703701. Click to request current manual
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NCBI/Uniprot data below describe general gene information for LEPR. It may not necessarily be applicable to this product.
 NCBI GI #    51093379
 NCBI GeneID    3953
 NCBI Accession #    NP_001003679.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001003679.3 [Other Products]
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 UniProt Primary Accession #    P48357 [Other Products]
 UniProt Secondary Accession #    Q13592; Q13593; Q13594; Q92919; Q92920; Q92921 [Other Products]
 UniProt Related Accession #    P48357 [Other Products]
 Molecular Weight    132,494 Da
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 NCBI Official Full Name    leptin receptor isoform 3
 NCBI Official Synonym Full Names    leptin receptor
 NCBI Official Symbol    LEPR [Similar Products]
 NCBI Official Synonym Symbols   
OBR; OB-R; CD295; LEP-R; LEPRD
[Similar Products]
 NCBI Protein Information    leptin receptor; huB219; OB receptor
 UniProt Protein Name    Leptin receptor
 UniProt Synonym Protein Names   
HuB219; OB receptor; OB-R; CD_antigen: CD295
 Protein Family    Leptin receptor
 UniProt Gene Name    LEPR [Similar Products]
 UniProt Synonym Gene Names    DB; OBR; LEP-R; OB-R [Similar Products]
 UniProt Entry Name    LEPR_HUMAN
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 NCBI Summary for LEPR    The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010]
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 UniProt Comments for LEPR    Function: Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway
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 Research Articles on LEPR    1. Increased placental leptin and leptin receptor expression may have a role in stimulating fetal overgrowth in type 1 diabetes pregnancy.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with LEPR elisa kitPathways associated with LEPR elisa kit
 Reference Product  PubMed Publications
 LEP elisa kit  >169 publications with LEPR and LEP
 STAT3 elisa kit  >50 publications with LEPR and STAT3
 JAK2 elisa kit  >12 publications with LEPR and JAK2
 SOCS3 elisa kit  >9 publications with LEPR and SOCS3
 IRS1 elisa kit  >8 publications with LEPR and IRS1
 INS elisa kit  >8 publications with LEPR and INS
 APOD elisa kit  >2 publications with LEPR and APOD
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Leptin Signaling Pathway antibodies  Leptin Signaling Pathway Diagram
Diseases associated with LEPR elisa kitOrgans/Tissues associated with LEPR elisa kit
 Disease Name  Pubmed Publications
 Peripheral Nervous System Diseases Antibodies  >401 publications with LEPR and Peripheral Nervous System Diseases
 Obesity Antibodies  >373 publications with LEPR and Obesity
 Drug-Related Side Effects and Adverse Reactions Antibodies  >349 publications with LEPR and Drug-Related Side Effects and Adverse Reactions
 Overweight Antibodies  >239 publications with LEPR and Overweight
 Diabetes Mellitus Antibodies  >209 publications with LEPR and Diabetes Mellitus
 Inflammation Antibodies  >198 publications with LEPR and Inflammation
 Diabetes Mellitus, Type 2 Antibodies  >119 publications with LEPR and Diabetes Mellitus, Type 2
 Insulin Resistance Antibodies  >110 publications with LEPR and Insulin Resistance
 Necrosis Antibodies  >110 publications with LEPR and Necrosis
 Immunologic Deficiency Syndromes Antibodies  >93 publications with LEPR and Immunologic Deficiency Syndromes
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with LEPR and Bone
 Connective Tissue Antibodies  >4 publications with LEPR and Connective Tissue
 Heart Antibodies  >3 publications with LEPR and Heart
 Muscle Antibodies  >3 publications with LEPR and Muscle
 Brain Antibodies  >3 publications with LEPR and Brain
 Vascular Antibodies  >2 publications with LEPR and Vascular
 Embryonic Tissue Antibodies  >2 publications with LEPR and Embryonic Tissue
 Ear Antibodies  >1 publications with LEPR and Ear
 Kidney Antibodies  >1 publications with LEPR and Kidney
 Umbilical Cord Antibodies  >1 publications with LEPR and Umbilical Cord
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