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HLA-G elisa kit :: Human leukocyte antigen G (HLA-G) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS267094
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $260 +1 FREE 8GB USB
96-Strip-Wells  /  $405 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

leukocyte antigen G (HLA-G), ELISA Kit

★Popular Item★
 Also Known As   

Human leukocyte antigen G (HLA-G) ELISA Kit

 Product Gene Name   

HLA-G elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for O02922
 Species Reactivity    Human
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 Specificity    No cross-reaction with other factors.
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 Samples    Serum, plasma or cell culture supernatant
 Assay Type    Quantitative Sandwich
 Detection Range    200 U/ml-3.12 U/ml
 Sensitivity    Up to 0.6U/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HLA-G elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HLA-G purchase    MBS267094 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the leukocyte antigen G (HLA-G) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 200 U/ml-3.12 U/ml in biological research samples containing HLA-G, with an estimated sensitivity of Up to 0.6U/ml. The ELISA analytical biochemical technique of the MBS267094 kit is based on HLA-G antibody-HLA-G antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HLA-G antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HLA-G. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma or cell culture supernatant. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Human leukocyte antigen G exists as a cell membrane localized and soluble human form. As a clinical hum immunol biomarker for placental development, research interest in expression and localization is an active area. Aiding these studies is an HLAG ELISA kit suitable for analysis of cells in culture, tissue homogenates and other samples requiring a broad detection range, including soluble HLA G. These HLAG ELISA kits are designed as double-sandwich format, unlike competitor products category members when ELISA kit select compare assay products is chosen. Adding to the antigen HLA kit 1 value are included kit 1 plate with HLA antibody 5a6g7, anti mouse IgG1 biotin secondary antibodies, standard protein product and either Alexa 174 fluor or AMP detection as described in plate datasheet. To purchase HLA antigen ELISA kit 1, to detect soluble human leukocyte antigen G, in the United States or other Republic countries and Islands, see MyBiosource.com
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Related Product Information for HLA-G elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human HLA-G monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of HLA-G elisa kit    HLA-G elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS267094. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HLA-G. It may not necessarily be applicable to this product.
 NCBI GI #    2117163
 NCBI GeneID    3105
 NCBI Accession #    CAA73716.1 [Other Products]
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 UniProt Primary Accession #    O02922 [Other Products]
 UniProt Related Accession #    P01891; P01892; P04439; P05534; P10314; P10316; P13746; P16189; P18462; P30443 [Other Products]
 Molecular Weight    29,434 Da
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 NCBI Official Full Name    leukocyte antigen, HLA-A2 variant, partial
 NCBI Official Synonym Full Names    major histocompatibility complex, class I, A
 NCBI Official Symbol    HLA-A [Similar Products]
 NCBI Official Synonym Symbols   
HLAA
[Similar Products]
 NCBI Protein Information    HLA class I histocompatibility antigen, A-1 alpha chain; antigen presenting molecule; leukocyte antigen class I-A; MHC class I antigen HLA-A heavy chain
 UniProt Protein Name    Leukocyte antigen, HLA-A2 variant
 UniProt Synonym Protein Names   
Leukocyte antigen, HLA-A2 variant
 Protein Family    HLA class I histocompatibility antigen
 UniProt Entry Name    O02922_HUMAN
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 NCBI Summary for HLA-G    HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]
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 UniProt Comments for HLA-G    HLAA: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; Golgi apparatus; phagocytic vesicle membrane; cell surface; membrane; integral to plasma membrane; early endosome membrane; endoplasmic reticulum; plasma membrane; MHC class I protein complex; Golgi medial cisterna

Molecular Function: protein binding; peptide antigen binding; beta-2-microglobulin binding; T cell receptor binding; TAP binding; receptor binding

Biological Process: regulation of immune response; viral reproduction; detection of bacterium; positive regulation of T cell mediated cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; protection from natural killer cell mediated cytotoxicity; regulation of defense response to virus by virus; positive regulation of interferon-gamma production; antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I; immune response

Disease: Severe Cutaneous Adverse Reaction, Susceptibility To
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 Research Articles on HLA-G    1. allele frequency is associated with progressive and non-progressive chronic lymphocytic leukemia in Iranian patients
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with HLA-G elisa kitOrgans/Tissues associated with HLA-G elisa kit
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway antibodies  Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing-Cross Presentation Pathway antibodies  Antigen Processing-Cross Presentation Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >7300 publications with HLA-G and Blood
 Kidney Antibodies  >1033 publications with HLA-G and Kidney
 Bone Antibodies  >949 publications with HLA-G and Bone
 Bone Marrow Antibodies  >889 publications with HLA-G and Bone Marrow
 Liver Antibodies  >485 publications with HLA-G and Liver
 Skin Antibodies  >438 publications with HLA-G and Skin
 Lung Antibodies  >391 publications with HLA-G and Lung
 Heart Antibodies  >213 publications with HLA-G and Heart
 Lymph Antibodies  >192 publications with HLA-G and Lymph
 Lymph Node Antibodies  >190 publications with HLA-G and Lymph Node
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