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LIG4 elisa kit :: Human ligase IV, DNA, ATP-dependent ELISA Kit

Scan QR to view Datasheet Catalog #    MBS9321742 LIG4 elisa kit
Unit / Price
48-Strip-Wells  /  $500 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,250 +4 FREE 8GB USB
10x96-Strip-Wells  /  $6,395 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ligase IV, DNA, ATP-dependent (LIG4), ELISA Kit

 Also Known As   

Human DNA ligase 4, LIG4 ELISA Kit

 Product Synonym Names    Human DNA ligase 4 (LIG4) ELISA kit; DNA joinase; DNA ligase (ATP) 4; DNA repair enzyme; polydeoxyribonucleotide synthase [ATP] 4; polynucleotide ligase; sealase; ligase IV; DNA; ATP-dependent
 Product Gene Name   

LIG4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    606593
 3D Structure    ModBase 3D Structure for P49917
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 Species Reactivity    Human
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 Specificity    No significant cross-reactivity or interference between this analyte and analogues is observed.
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 Samples    Undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
 Assay Type    Sandwich
 Detection Range    0.625ng/ml-20ng/ml.
 Sensitivity    0.1ng/ml.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100].
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of LIG4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for LIG4 purchase    MBS9321742 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ligase IV, DNA, ATP-dependent (LIG4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625ng/ml-20ng/ml in biological research samples containing LIG4, with an estimated sensitivity of 0.1ng/ml. The ELISA analytical biochemical technique of the MBS9321742 kit is based on LIG4 antibody-LIG4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LIG4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LIG4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for LIG4 elisa kit

   Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, NOT for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of LIG4 (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Sample Manual Insert of MBS9321742. Click to request current manual
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NCBI/Uniprot data below describe general gene information for LIG4. It may not necessarily be applicable to this product.
 NCBI GI #    148539894
 NCBI GeneID    3981
 NCBI Accession #    NP_001091738.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001098268.1 [Other Products]
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 UniProt Primary Accession #    P49917 [Other Products]
 UniProt Secondary Accession #    Q8IY66; Q8TEU5 [Other Products]
 UniProt Related Accession #    P49917 [Other Products]
 Molecular Weight    103,971 Da
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 NCBI Official Full Name    DNA ligase 4
 NCBI Official Synonym Full Names    ligase IV, DNA, ATP-dependent
 NCBI Official Symbol    LIG4 [Similar Products]
 NCBI Official Synonym Symbols   
LIG4S
[Similar Products]
 NCBI Protein Information    DNA ligase 4; sealase; DNA joinase; DNA ligase IV; DNA repair enzyme; polynucleotide ligase; polydeoxyribonucleotide synthase [ATP] 4
 UniProt Protein Name    DNA ligase 4
 UniProt Synonym Protein Names   
DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4
 Protein Family    DNA ligase
 UniProt Gene Name    LIG4 [Similar Products]
 UniProt Entry Name    DNLI4_HUMAN
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 NCBI Summary for LIG4    The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
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 UniProt Comments for LIG4    LIG4: a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. Defects in this gene are the cause of LIG4 syndrome.

Protein type: Ligase; EC 6.5.1.1

Chromosomal Location of Human Ortholog: 13q33-q34

Cellular Component: nucleoplasm; DNA ligase IV complex; focal adhesion; cytoplasm; plasma membrane; condensed chromosome; DNA-dependent protein kinase complex; nucleus

Molecular Function: protein C-terminus binding; protein binding; DNA binding; DNA ligase activity; DNA ligase (ATP) activity; metal ion binding; ATP binding; ligase activity

Biological Process: central nervous system development; viral reproduction; somatic stem cell maintenance; T cell differentiation in the thymus; chromosome organization and biogenesis; double-strand break repair via nonhomologous end joining; DNA ligation; positive regulation of fibroblast proliferation; double-strand break repair; response to gamma radiation; DNA ligation during DNA repair; DNA ligation during DNA recombination; negative regulation of neuron apoptosis; response to X-ray; single strand break repair; pro-B cell differentiation; positive regulation of neurogenesis; V(D)J recombination; immunoglobulin V(D)J recombination; in utero embryonic development; isotype switching; cell cycle; DNA repair; lagging strand elongation; cell proliferation; neuron apoptosis; cell division; T cell receptor V(D)J recombination; nucleotide-excision repair, DNA gap filling

Disease: Myeloma, Multiple; Lig4 Syndrome
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 Research Articles on LIG4    1. study reports the identification of biallelic truncating LIG4 mutations in 11 patients with microcephalic primordial dwarfism presenting with restricted prenatal growth and extreme postnatal global growth failure
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with LIG4 elisa kitPathways associated with LIG4 elisa kit
 Reference Product  PubMed Publications
 XRCC4 elisa kit  >30 publications with LIG4 and XRCC4
 PRKDC elisa kit  >9 publications with LIG4 and PRKDC
 NHEJ1 elisa kit  >2 publications with LIG4 and NHEJ1
 Products by Pathway  Pathway Diagram
 2-LTR Circle Formation Pathway antibodies  2-LTR Circle Formation Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Double-Strand Break Repair Pathway antibodies  Double-Strand Break Repair Pathway Diagram
 Early Phase Of HIV Life Cycle Pathway antibodies  Early Phase Of HIV Life Cycle Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
 HIV Life Cycle Pathway antibodies  HIV Life Cycle Pathway Diagram
 Integration Of Provirus Pathway antibodies  Integration Of Provirus Pathway Diagram
 Non-homologous End-joining Pathway antibodies  Non-homologous End-joining Pathway Diagram
 Non-homologous End-joining Pathway antibodies  Non-homologous End-joining Pathway Diagram
Diseases associated with LIG4 elisa kitOrgans/Tissues associated with LIG4 elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >35 publications with LIG4 and Neoplasms
 LIG4 SYNDROME Antibodies  >26 publications with LIG4 and LIG4 SYNDROME
 Skin Diseases Antibodies  >10 publications with LIG4 and Skin Diseases
 Nervous System Diseases Antibodies  >9 publications with LIG4 and Nervous System Diseases
 Lymphoma Antibodies  >8 publications with LIG4 and Lymphoma
 Carcinoma Antibodies  >7 publications with LIG4 and Carcinoma
 Leukemia Antibodies  >6 publications with LIG4 and Leukemia
 Urogenital Neoplasms Antibodies  >6 publications with LIG4 and Urogenital Neoplasms
 Drug Toxicity Antibodies  >5 publications with LIG4 and Drug Toxicity
 Neoplasms, Experimental Antibodies  >5 publications with LIG4 and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >23 publications with LIG4 and Blood
 Bone Antibodies  >7 publications with LIG4 and Bone
 Brain Antibodies  >7 publications with LIG4 and Brain
 Skin Antibodies  >4 publications with LIG4 and Skin
 Muscle Antibodies  >2 publications with LIG4 and Muscle
 Pharynx Antibodies  >2 publications with LIG4 and Pharynx
 Prostate Antibodies  >2 publications with LIG4 and Prostate
 Thymus Antibodies  >2 publications with LIG4 and Thymus
 Kidney Antibodies  >1 publications with LIG4 and Kidney
 Testis Antibodies  >1 publications with LIG4 and Testis
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