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MMP1 elisa kit :: Human matrix metallopeptidase 1 (interstitial collagenase) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS703279
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $375 +1 FREE 8GB USB
96-Strip-Wells  /  $535 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,295 +2 FREE 8GB USB
10x96-Strip-Wells  /  $4,330 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

matrix metallopeptidase 1 (interstitial collagenase) (MMP1), ELISA Kit

★Popular Item★
 Also Known As   

Human Matrix metalloproteinase 1, MMP-1 ELISA Kit

 Product Synonym Names    Human Matrix metalloproteinase 1; MMP-1 ELISA kit; CLG; CLGN; fibroblast collagenase; matrix metalloprotease 1; matrix metalloproteinase 1; matrix metallopeptidase 1 (interstitial collagenase)
 Product Gene Name   

MMP1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS703279 Testing Data    Testing Data PDF
 OMIM    120353
 3D Structure    ModBase 3D Structure for P03956
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human MMP1. No significant cross-reactivity or interference between Human MMP1 and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, tissue homogenates
 Assay Type    Sandwich
 Detection Range    0.312 ng/ml-20 ng/ml
 Sensitivity    0.078 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Developmental Protein
 Protein Biological Process 3    Collagen degradation
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MMP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MMP1 purchase    MBS703279 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the matrix metallopeptidase 1 (interstitial collagenase) (MMP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312 ng/ml-20 ng/ml in biological research samples containing MMP1, with an estimated sensitivity of 0.078 ng/ml. The ELISA analytical biochemical technique of the MBS703279 kit is based on MMP1 antibody-MMP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MMP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MMP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MMP1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of MMP1 elisa kit    MMP1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS703279. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MMP1. It may not necessarily be applicable to this product.
 NCBI GI #    4505215
 NCBI GeneID    4312
 NCBI Accession #    NP_002412.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002421.3 [Other Products]
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 UniProt Primary Accession #    P03956 [Other Products]
 UniProt Secondary Accession #    P08156 [Other Products]
 UniProt Related Accession #    P03956 [Other Products]
 Molecular Weight    54,007 Da
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 NCBI Official Full Name    interstitial collagenase isoform 1 preproprotein
 NCBI Official Synonym Full Names    matrix metallopeptidase 1 (interstitial collagenase)
 NCBI Official Symbol    MMP1 [Similar Products]
 NCBI Official Synonym Symbols   
CLG; CLGN
[Similar Products]
 NCBI Protein Information    interstitial collagenase; fibroblast collagenase; matrix metalloprotease 1; matrix metalloproteinase 1
 UniProt Protein Name    Interstitial collagenase
 UniProt Synonym Protein Names   
Fibroblast collagenase; Matrix metalloproteinase-1; MMP-1
 Protein Family    Interstitial collagenase
 UniProt Gene Name    MMP1 [Similar Products]
 UniProt Synonym Gene Names    CLG; MMP-1 [Similar Products]
 UniProt Entry Name    MMP1_HUMAN
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 NCBI Summary for MMP1    Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]
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 UniProt Comments for MMP1    Function: Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.1 PublicationManual assertion based on experiment in:Ref.12

Catalytic activity: Cleavage of the triple helix of collagen at about three-quarters of the length of the molecule from the N-terminus, at 775-Gly-|-Ile-776 in the alpha-1(I) chain. Cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue.

Cofactor: Binds 4 calcium ions per subunit.

Enzyme regulation: Can be activated without removal of the activation peptide.
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 Research Articles on MMP1    1. Matrix metalloproteinase-1 promoter -1607 1G/2G (rs1799750) polymorphism may have no effect on disease susceptibility of CP in Caucasian, Asian and Brazilian mixed populations
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MMP1 elisa kitPathways associated with MMP1 elisa kit
 Reference Product  PubMed Publications
 JUN elisa kit  >144 publications with MMP1 and JUN
 TIMP1 elisa kit  >137 publications with MMP1 and TIMP1
 MMP7 elisa kit  >59 publications with MMP1 and MMP7
 FOS elisa kit  >28 publications with MMP1 and FOS
 ETS1 elisa kit  >20 publications with MMP1 and ETS1
 BSG elisa kit  >5 publications with MMP1 and BSG
 IGFBP3 elisa kit  >2 publications with MMP1 and IGFBP3
 FOSL1 elisa kit  >1 publications with MMP1 and FOSL1
 Products by Pathway  Pathway Diagram
 Activation Of Matrix Metalloproteinases Pathway antibodies  Activation Of Matrix Metalloproteinases Pathway Diagram
 Basigin Interactions Pathway antibodies  Basigin Interactions Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Collagen Degradation Pathway antibodies  Collagen Degradation Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Endothelins Pathway antibodies  Endothelins Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
Diseases associated with MMP1 elisa kitOrgans/Tissues associated with MMP1 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >119 publications with MMP1 and Inflammation
 Cardiovascular Diseases Antibodies  >87 publications with MMP1 and Cardiovascular Diseases
 Fibrosis Antibodies  >83 publications with MMP1 and Fibrosis
 Neoplasm Invasiveness Antibodies  >81 publications with MMP1 and Neoplasm Invasiveness
 Lung Diseases Antibodies  >75 publications with MMP1 and Lung Diseases
 Necrosis Antibodies  >74 publications with MMP1 and Necrosis
 Neoplasm Metastasis Antibodies  >65 publications with MMP1 and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >56 publications with MMP1 and Breast Neoplasms
 Lung Neoplasms Antibodies  >42 publications with MMP1 and Lung Neoplasms
 Heart Diseases Antibodies  >38 publications with MMP1 and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with MMP1 and Bone
 Connective Tissue Antibodies  >4 publications with MMP1 and Connective Tissue
 Heart Antibodies  >3 publications with MMP1 and Heart
 Muscle Antibodies  >3 publications with MMP1 and Muscle
 Brain Antibodies  >3 publications with MMP1 and Brain
 Vascular Antibodies  >2 publications with MMP1 and Vascular
 Embryonic Tissue Antibodies  >2 publications with MMP1 and Embryonic Tissue
 Ear Antibodies  >1 publications with MMP1 and Ear
 Kidney Antibodies  >1 publications with MMP1 and Kidney
 Umbilical Cord Antibodies  >1 publications with MMP1 and Umbilical Cord
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