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MAGED4B elisa kit :: Human melanoma antigen family D, 4B ELISA Kit

Scan QR to view Datasheet Catalog #    MBS701702
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

melanoma antigen family D, 4B (MAGED4B), ELISA Kit

 Also Known As   

Human Melanoma-associated antigen D4, MAGED4/MAGED4B ELISA Kit

 Product Synonym Names    Human Melanoma-associated antigen D4 (MAGED4/MAGED4B) ELISA Kit; melanoma antigen family D; 4B
 Product Gene Name   

MAGED4B elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS701702 Testing Data    Testing Data PDF
 OMIM    gene 300765
 3D Structure    ModBase 3D Structure for Q96JG8
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human MAGED4B. No significant cross-reactivity or interference between Human MAGED4B and analogues was observed.
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 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MAGED4B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MAGED4B purchase    MBS701702 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the melanoma antigen family D, 4B (MAGED4B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MAGED4B. The ELISA analytical biochemical technique of the MBS701702 kit is based on MAGED4B antibody-MAGED4B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MAGED4B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MAGED4B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MAGED4B elisa kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAGED4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for MAGED4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain MAGED4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MAGED4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of MAGED4B elisa kit    MAGED4B elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS701702. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MAGED4B. It may not necessarily be applicable to this product.
 NCBI GI #    149274614
 NCBI GeneID    728239
 NCBI Accession #    NP_001092270.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001098800.2 [Other Products]
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 UniProt Primary Accession #    Q96JG8 [Other Products]
 UniProt Secondary Accession #    Q5HYN6; Q8WXW4; Q9BQ84; Q9BVH1; Q9BYH3; Q9BYH4; Q9H217; A8K093 [Other Products]
 UniProt Related Accession #    Q96JG8 [Other Products]
 Molecular Weight    81,378 Da
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 NCBI Official Full Name    melanoma-associated antigen D4 isoform 2
 NCBI Official Synonym Full Names    melanoma antigen family D, 4
 NCBI Official Symbol    MAGED4 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    melanoma-associated antigen D4; MAGE-D4 antigen; MAGE-E1 antigen
 UniProt Protein Name    Melanoma-associated antigen D4
 UniProt Synonym Protein Names   
MAGE-D4 antigen; MAGE-E1 antigen
 UniProt Gene Name    MAGED4 [Similar Products]
 UniProt Synonym Gene Names    KIAA1859; MAGED4A; MAGEE1
[Similar Products]
 UniProt Entry Name    MAGD4_HUMAN
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 UniProt Comments for MAGED4B    Function: May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.1 PublicationManual assertion based on experiment in:Ref.13
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with MAGED4B elisa kitDiseases associated with MAGED4B elisa kit
 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Regulation Of Apoptosis Pathway antibodies  Regulation Of Apoptosis Pathway Diagram
 Role Of DCC In Regulating Apoptosis Pathway antibodies  Role Of DCC In Regulating Apoptosis Pathway Diagram
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >1 publications with MAGED4B and Kidney Diseases
 Adenocarcinoma Antibodies  >1 publications with MAGED4B and Adenocarcinoma
 Nervous System Diseases Antibodies  >1 publications with MAGED4B and Nervous System Diseases
Organs/Tissues associated with MAGED4B elisa kit
 Organ/Tissue Name  Pubmed Publications
 Gonad Antibodies  >111 publications with MAGED4B and Gonad
 Whole Body Antibodies  >3 publications with MAGED4B and Whole Body
 Fat Body Antibodies  >2 publications with MAGED4B and Fat Body
 Uncharacterized Tissue Antibodies  >1 publications with MAGED4B and Uncharacterized Tissue
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